tetano
Editor, Senior Moderator
J Microbiol. 2013 Dec;51(6):836-43. doi: 10.1007/s12275-013-3521-y. Epub 2013 Dec 19.
Cyclic dipeptides from lactic acid bacteria inhibit proliferation of the influenza a virus.
Kwak MK, Liu R, Kwon JO, Kim MK, Kim AH, Kang SO.
Author information
Abstract
We isolated Lactobacillus plantarum LBP-K10 from the traditional Korean fermented food kimchi. When organic acids were removed, the culture filtrate of this isolate showed high antiviral activity (measured using a plaque-forming assay) against the influenza A (H3N2) virus. Two fractions that were active against influenza A virus were purified from the culture filtrate using a C18 column with high-performance liquid chromatography. These active fractions were crystallized and identified to be the cyclic dipeptides cis-cyclo (L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro) using gas chromatography-mass spectrometry; this identification was confirmed by X-ray crystallography. These cyclic dipeptides were identified in the culture filtrate of other lactic acid bacteria, including Lactobacillus spp., Leuconostoc spp., Weissella spp., and Lactococcus lactis.
PMID:
24385362
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24385362
Cyclic dipeptides from lactic acid bacteria inhibit proliferation of the influenza a virus.
Kwak MK, Liu R, Kwon JO, Kim MK, Kim AH, Kang SO.
Author information
Abstract
We isolated Lactobacillus plantarum LBP-K10 from the traditional Korean fermented food kimchi. When organic acids were removed, the culture filtrate of this isolate showed high antiviral activity (measured using a plaque-forming assay) against the influenza A (H3N2) virus. Two fractions that were active against influenza A virus were purified from the culture filtrate using a C18 column with high-performance liquid chromatography. These active fractions were crystallized and identified to be the cyclic dipeptides cis-cyclo (L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro) using gas chromatography-mass spectrometry; this identification was confirmed by X-ray crystallography. These cyclic dipeptides were identified in the culture filtrate of other lactic acid bacteria, including Lactobacillus spp., Leuconostoc spp., Weissella spp., and Lactococcus lactis.
PMID:
24385362
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24385362