tetano
Editor, Senior Moderator
Curr Mol Med
. 2022 Nov 4.
doi: 10.2174/1566524023666221104160148. Online ahead of print.
Direct SARS-CoV-2 Detection System Utilizing Simple-to-Use Capillary Gel Electrophoresis Sample-to-Result
Neo Yang[SUP] 1 [/SUP], Meya Kuo[SUP] 1 [/SUP], Jerry Liu[SUP] 1 [/SUP], Varoujan Amirkhanian[SUP] 1 [/SUP], Eric Tsai[SUP] 1 [/SUP]
Affiliations
Abstract
We present a Direct SARS-CoV-2 Detection System that achieves sample-to-results in less than two hours in three simple steps. The Detection System includes Direct one-step Reverse Transcription PCR (RT-PCR) reagents (Qexp-MDx kit), a portable thermal cycler (Qamp-mini) with a pre-programmed chip and a simple-to-use Capillary Gel Electrophoresis system (Qsep Series Bio-Fragment Analyzer) with high fluorescence detection sensitivity to solve the problems associated with traditional real-time PCR (qPCR) systems which produces inaccurate test results with high false negative and false positive rates. The proposed simple-to-use detection platform can provide high detection sensitivity (identify less than 20 copies), fast results (less than 120 minutes) and cost-effective results which should be suitable for decentralized testing application of COVID-19.
Keywords: COVID-19; COVID-1Polymerase Chain Reaction (PCR); Capillary Electrophoresis (CE); Coronavirus; Fragment Analyzer; Gel Electrophoresis; Pandemic; Polymerase Chain Reaction (PCR); Reverse Transcription PCR (RT-PCR); SARS-CoV-2.
. 2022 Nov 4.
doi: 10.2174/1566524023666221104160148. Online ahead of print.
Direct SARS-CoV-2 Detection System Utilizing Simple-to-Use Capillary Gel Electrophoresis Sample-to-Result
Neo Yang[SUP] 1 [/SUP], Meya Kuo[SUP] 1 [/SUP], Jerry Liu[SUP] 1 [/SUP], Varoujan Amirkhanian[SUP] 1 [/SUP], Eric Tsai[SUP] 1 [/SUP]
Affiliations
- PMID: 36336803
- DOI: 10.2174/1566524023666221104160148
Abstract
We present a Direct SARS-CoV-2 Detection System that achieves sample-to-results in less than two hours in three simple steps. The Detection System includes Direct one-step Reverse Transcription PCR (RT-PCR) reagents (Qexp-MDx kit), a portable thermal cycler (Qamp-mini) with a pre-programmed chip and a simple-to-use Capillary Gel Electrophoresis system (Qsep Series Bio-Fragment Analyzer) with high fluorescence detection sensitivity to solve the problems associated with traditional real-time PCR (qPCR) systems which produces inaccurate test results with high false negative and false positive rates. The proposed simple-to-use detection platform can provide high detection sensitivity (identify less than 20 copies), fast results (less than 120 minutes) and cost-effective results which should be suitable for decentralized testing application of COVID-19.
Keywords: COVID-19; COVID-1Polymerase Chain Reaction (PCR); Capillary Electrophoresis (CE); Coronavirus; Fragment Analyzer; Gel Electrophoresis; Pandemic; Polymerase Chain Reaction (PCR); Reverse Transcription PCR (RT-PCR); SARS-CoV-2.