tetano
Editor, Senior Moderator
Antiviral Res. 2014 Dec 13. pii: S0166-3542(14)00359-3. doi: 10.1016/j.antiviral.2014.12.008. [Epub ahead of print]
[h=1]Cross-reactive immunity against influenza viruses in children and adults following 2009 pandemic H1N1 infection.[/h] Ahmed MS[SUP]1[/SUP], Jacques LC[SUP]1[/SUP], Mahallawi W[SUP]1[/SUP], Ferrara F[SUP]2[/SUP], Temperton N[SUP]2[/SUP], Upile N[SUP]3[/SUP], Vaughan C[SUP]3[/SUP], Sharma R[SUP]3[/SUP], Beer H[SUP]4[/SUP], Hoschler K[SUP]5[/SUP], McNamara PS[SUP]6[/SUP], Zhang Q[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] 2009 H1N1 pandemic influenza (A(H1N1)pdm09) virus infected large numbers of people worldwide. Recent studies suggest infection with A(H1N1)pdm09 virus elicited cross-reactive anti-hemagglutinin (HA) memory B cell response to conserved regions of HA. However, the breadth and magnitude of cross-reactive immunity in children and adults following A(H1N1)pdm09 infection are unknown. Methods: We investigated serum anti-HA immunity to a number of group-1 and -2 viruses in children and adults using hemagglutination inhibition (HAI), enzyme-linked immunosorbent assay and virus neutralization assay. Results: Applying hemagglutination inhibition (HAI) titres⩾40 against A(H1N1)pdm09 as threshold of sero-positivity, we observed significantly higher levels of anti-HA antibodies to a number of virus subtypes, including those neutralizing H5N1, in subjects with HAI titre⩾40 than those with HAI<40. Adults demonstrated broader and stronger cross-reactive anti-HA antibodies than children, including cross-reactive anti-HA1 and -HA2 antibodies. By comparison, individuals with serologic evidence of recent exposure to seasonal H1N1 or H3N2 did not show such broad cross-reactive immunity. Conclusion: Our results suggest individuals exposed to A(H1N1)pdm09 virus developed a broad and age-associated cross-reactive anti-HA immunity which may have important implications for future vaccination strategies to enable protection against a broader range of influenza viruses.
Copyright ? 2014. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Anti-hemagglutinin antibody; Cross-reactive immunity; Influenza virus; Pandemic H1N1 infection; Serum antibody; Virus neutralization
PMID: 25513756 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25513756
[h=1]Cross-reactive immunity against influenza viruses in children and adults following 2009 pandemic H1N1 infection.[/h] Ahmed MS[SUP]1[/SUP], Jacques LC[SUP]1[/SUP], Mahallawi W[SUP]1[/SUP], Ferrara F[SUP]2[/SUP], Temperton N[SUP]2[/SUP], Upile N[SUP]3[/SUP], Vaughan C[SUP]3[/SUP], Sharma R[SUP]3[/SUP], Beer H[SUP]4[/SUP], Hoschler K[SUP]5[/SUP], McNamara PS[SUP]6[/SUP], Zhang Q[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] 2009 H1N1 pandemic influenza (A(H1N1)pdm09) virus infected large numbers of people worldwide. Recent studies suggest infection with A(H1N1)pdm09 virus elicited cross-reactive anti-hemagglutinin (HA) memory B cell response to conserved regions of HA. However, the breadth and magnitude of cross-reactive immunity in children and adults following A(H1N1)pdm09 infection are unknown. Methods: We investigated serum anti-HA immunity to a number of group-1 and -2 viruses in children and adults using hemagglutination inhibition (HAI), enzyme-linked immunosorbent assay and virus neutralization assay. Results: Applying hemagglutination inhibition (HAI) titres⩾40 against A(H1N1)pdm09 as threshold of sero-positivity, we observed significantly higher levels of anti-HA antibodies to a number of virus subtypes, including those neutralizing H5N1, in subjects with HAI titre⩾40 than those with HAI<40. Adults demonstrated broader and stronger cross-reactive anti-HA antibodies than children, including cross-reactive anti-HA1 and -HA2 antibodies. By comparison, individuals with serologic evidence of recent exposure to seasonal H1N1 or H3N2 did not show such broad cross-reactive immunity. Conclusion: Our results suggest individuals exposed to A(H1N1)pdm09 virus developed a broad and age-associated cross-reactive anti-HA immunity which may have important implications for future vaccination strategies to enable protection against a broader range of influenza viruses.
Copyright ? 2014. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Anti-hemagglutinin antibody; Cross-reactive immunity; Influenza virus; Pandemic H1N1 infection; Serum antibody; Virus neutralization
PMID: 25513756 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25513756