tetano
Editor, Senior Moderator
J Infect Dis. 2016 Oct 12. pii: jiw471. [Epub ahead of print]
[h=1]Cross-Immunities against Avian Influenza H7N9 Virus in the Healthy Population Affected by Antigenicity-Dependent Substitutions.[/h] Liu WJ[SUP]1[/SUP], Tan S[SUP]2[/SUP], Zhao M[SUP]2[/SUP], Quan C[SUP]3[/SUP], Bi Y[SUP]4[/SUP], Wu Y[SUP]4[/SUP], Zhang S[SUP]4[/SUP], Zhang H[SUP]5[/SUP], Xiao H[SUP]6[/SUP], Qi J[SUP]4[/SUP], Yan J[SUP]4[/SUP], Liu W[SUP]4[/SUP], Yu H[SUP]7[/SUP], Shu Y[SUP]3[/SUP], Wu G[SUP]3[/SUP], Gao GF[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The emergence of infections by the novel avian influenza A (H7N9) virus has posed a threat to human health. Cross-immunity between H7N9 and other heterosubtypic influenza viruses affected by antigenicity-dependent substitutions needs to be investigated.
[h=4]METHODS:[/h] We investigated the cellular and humoral immune responses against H7N9 and the 2009 pandemic H1N1 influenza viruses, by serological and T-cell-specific assays in a healthy population. The molecular bases of the cellular and humoral antigenic variability of H7N9 were illuminated by structural determination.
[h=4]RESULTS:[/h] We not only found antibodies against H7N9 were lacking in the studied population, but also revealed both CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cells that cross-reacted with H7N9 were at significantly lower levels than those against the 2009pH1N1 peptides with substitutions. Moreover, individual peptides for the H7N9 virus with low cross-reactivity were identified. Structural determination indicated that substitutions within these peptides influence the antigenic variability of H7N9 through both major histocompatibility complex (MHC)-binding and T-cell receptor (TCR)-docking.
[h=4]CONCLUSIONS:[/h] The impact of antigenicity-dependent substitutions on cross-reactivity of T-cell immunity against the novel H7N9 virus in the healthy population benefits the understanding of immune evasion of influenza viruses and provides a useful reference for universal vaccine development.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.
PMID: 27738054 DOI: 10.1093/infdis/jiw471
[PubMed - as supplied by publisher]
[h=1]Cross-Immunities against Avian Influenza H7N9 Virus in the Healthy Population Affected by Antigenicity-Dependent Substitutions.[/h] Liu WJ[SUP]1[/SUP], Tan S[SUP]2[/SUP], Zhao M[SUP]2[/SUP], Quan C[SUP]3[/SUP], Bi Y[SUP]4[/SUP], Wu Y[SUP]4[/SUP], Zhang S[SUP]4[/SUP], Zhang H[SUP]5[/SUP], Xiao H[SUP]6[/SUP], Qi J[SUP]4[/SUP], Yan J[SUP]4[/SUP], Liu W[SUP]4[/SUP], Yu H[SUP]7[/SUP], Shu Y[SUP]3[/SUP], Wu G[SUP]3[/SUP], Gao GF[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The emergence of infections by the novel avian influenza A (H7N9) virus has posed a threat to human health. Cross-immunity between H7N9 and other heterosubtypic influenza viruses affected by antigenicity-dependent substitutions needs to be investigated.
[h=4]METHODS:[/h] We investigated the cellular and humoral immune responses against H7N9 and the 2009 pandemic H1N1 influenza viruses, by serological and T-cell-specific assays in a healthy population. The molecular bases of the cellular and humoral antigenic variability of H7N9 were illuminated by structural determination.
[h=4]RESULTS:[/h] We not only found antibodies against H7N9 were lacking in the studied population, but also revealed both CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cells that cross-reacted with H7N9 were at significantly lower levels than those against the 2009pH1N1 peptides with substitutions. Moreover, individual peptides for the H7N9 virus with low cross-reactivity were identified. Structural determination indicated that substitutions within these peptides influence the antigenic variability of H7N9 through both major histocompatibility complex (MHC)-binding and T-cell receptor (TCR)-docking.
[h=4]CONCLUSIONS:[/h] The impact of antigenicity-dependent substitutions on cross-reactivity of T-cell immunity against the novel H7N9 virus in the healthy population benefits the understanding of immune evasion of influenza viruses and provides a useful reference for universal vaccine development.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.
PMID: 27738054 DOI: 10.1093/infdis/jiw471
[PubMed - as supplied by publisher]