tetano
Editor, Senior Moderator
Int J Environ Res Public Health. 2015 Jan 20;12(1):1020-8. doi: 10.3390/ijerph120101020.
[h=1]Could A Deletion in Neuraminidase Stalk Strengthen Human Tropism of the Novel Avian Influenza Virus H7N9 in China, 2013?[/h] Chen L[SUP]1[/SUP], Zhu F[SUP]2[/SUP], Xiong C[SUP]3[/SUP], Zhang Z[SUP]4[/SUP], Jiang L[SUP]5[/SUP], Chen Y[SUP]6[/SUP], Zhao G[SUP]7[/SUP], Jiang Q[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Objective. A novel avian influenza A virus (AIV) H7N9 subtype which emerged in China in 2013 caused worldwide concern. Deletion of amino-acids 69 to 73 in the neuraminidase stalk was its most notable characteristic. This study is aimed to discuss the tropism and virulence effects of this deletion.
[h=4]METHODS:[/h] Neuraminidase gene sequences of N9 subtype were collected from NCBI and GISAID. MEGA6.0, Stata12.0, and UCSF Chimera were employed for sequence aligning, significance testing, and protein tertiary structure homology modeling.
[h=4]RESULTS:[/h] A total of 736 sequences were obtained; there were 81 human isolates of the novel AIV H7N9, of which 79 had the deletion. Among all the 654 avian origin sequences, only 43 had the deletion (p < 0.001). Tertiary structure displayed that the deletion obviously changed the spatial direction of neuraminidase.
[h=4]CONCLUSIONS:[/h] The deletion in neuraminidase stalk could have strengthened human tropism of the novel AIV H7N9, as well as its virulence.
PMID: 25608590 [PubMed - in process] Free full text
[h=1]Could A Deletion in Neuraminidase Stalk Strengthen Human Tropism of the Novel Avian Influenza Virus H7N9 in China, 2013?[/h] Chen L[SUP]1[/SUP], Zhu F[SUP]2[/SUP], Xiong C[SUP]3[/SUP], Zhang Z[SUP]4[/SUP], Jiang L[SUP]5[/SUP], Chen Y[SUP]6[/SUP], Zhao G[SUP]7[/SUP], Jiang Q[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Objective. A novel avian influenza A virus (AIV) H7N9 subtype which emerged in China in 2013 caused worldwide concern. Deletion of amino-acids 69 to 73 in the neuraminidase stalk was its most notable characteristic. This study is aimed to discuss the tropism and virulence effects of this deletion.
[h=4]METHODS:[/h] Neuraminidase gene sequences of N9 subtype were collected from NCBI and GISAID. MEGA6.0, Stata12.0, and UCSF Chimera were employed for sequence aligning, significance testing, and protein tertiary structure homology modeling.
[h=4]RESULTS:[/h] A total of 736 sequences were obtained; there were 81 human isolates of the novel AIV H7N9, of which 79 had the deletion. Among all the 654 avian origin sequences, only 43 had the deletion (p < 0.001). Tertiary structure displayed that the deletion obviously changed the spatial direction of neuraminidase.
[h=4]CONCLUSIONS:[/h] The deletion in neuraminidase stalk could have strengthened human tropism of the novel AIV H7N9, as well as its virulence.
PMID: 25608590 [PubMed - in process] Free full text