tetano
Editor, Senior Moderator
Sensors (Basel). 2017 Nov 11;17(11). pii: E2597. doi: 10.3390/s17112597.
[h=1]Cost-Effective and Handmade Paper-Based Immunosensing Device for Electrochemical Detection of Influenza Virus.[/h] Devarakonda S[SUP]1[/SUP], Singh R[SUP]2[/SUP], Bhardwaj J[SUP]3[/SUP], Jang J[SUP]4,[/SUP][SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Although many studies concerning the detection of influenza virus have been published, a paper-based, label-free electrochemical immunosensor has never been reported. Here, we present a cost-effective, handmade paper-based immunosensor for label-free electrochemical detection of influenza virus H1N1. This immunosensor was prepared by modifying paper with a spray of hydrophobic silica nanoparticles, and using stencil-printed electrodes. We used a glass vaporizer to spray the hydrophobic silica nanoparticles onto the paper, rendering it super-hydrophobic. The super-hydrophobicity, which is essential for this paper-based biosensor, was achieved via 30-40 spray coatings, corresponding to a 0.39-0.41 mg cm[SUP]-2[/SUP] coating of nanoparticles on the paper and yielding a water contact angle of 150? ? 1?. Stencil-printed carbon electrodes modified with single-walled carbon nanotubes and chitosan were employed to increase the sensitivity of the sensor, and the antibodies were immobilized via glutaraldehyde cross-linking. Differential pulse voltammetry was used to assess the sensitivity of the sensors at various virus concentrations, ranging from 10 to 10⁴ PFU mL[SUP]-1[/SUP], and the selectivity was assessed against MS2 bacteriophages and the influenza B viruses. These immunosensors showed good linear behaviors, improved detection times (30 min), and selectivity for the H1N1 virus with a limit of detection of 113 PFU mL[SUP]-1[/SUP], which is sufficiently sensitive for rapid on-site diagnosis. The simple and inexpensive methodologies developed in this study have great potential to be used for the development of a low-cost and disposable immunosensor for detection of pathogenic microorganisms, especially in developing countries.
[h=4]KEYWORDS:[/h] carbon nanotubes; electrochemical immunosensor; influenza virus; label-free detection; paper sensor; silica nanoparticles; stencil printing
PMID: 29137115 DOI: 10.3390/s17112597
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[h=1]Cost-Effective and Handmade Paper-Based Immunosensing Device for Electrochemical Detection of Influenza Virus.[/h] Devarakonda S[SUP]1[/SUP], Singh R[SUP]2[/SUP], Bhardwaj J[SUP]3[/SUP], Jang J[SUP]4,[/SUP][SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Although many studies concerning the detection of influenza virus have been published, a paper-based, label-free electrochemical immunosensor has never been reported. Here, we present a cost-effective, handmade paper-based immunosensor for label-free electrochemical detection of influenza virus H1N1. This immunosensor was prepared by modifying paper with a spray of hydrophobic silica nanoparticles, and using stencil-printed electrodes. We used a glass vaporizer to spray the hydrophobic silica nanoparticles onto the paper, rendering it super-hydrophobic. The super-hydrophobicity, which is essential for this paper-based biosensor, was achieved via 30-40 spray coatings, corresponding to a 0.39-0.41 mg cm[SUP]-2[/SUP] coating of nanoparticles on the paper and yielding a water contact angle of 150? ? 1?. Stencil-printed carbon electrodes modified with single-walled carbon nanotubes and chitosan were employed to increase the sensitivity of the sensor, and the antibodies were immobilized via glutaraldehyde cross-linking. Differential pulse voltammetry was used to assess the sensitivity of the sensors at various virus concentrations, ranging from 10 to 10⁴ PFU mL[SUP]-1[/SUP], and the selectivity was assessed against MS2 bacteriophages and the influenza B viruses. These immunosensors showed good linear behaviors, improved detection times (30 min), and selectivity for the H1N1 virus with a limit of detection of 113 PFU mL[SUP]-1[/SUP], which is sufficiently sensitive for rapid on-site diagnosis. The simple and inexpensive methodologies developed in this study have great potential to be used for the development of a low-cost and disposable immunosensor for detection of pathogenic microorganisms, especially in developing countries.
[h=4]KEYWORDS:[/h] carbon nanotubes; electrochemical immunosensor; influenza virus; label-free detection; paper sensor; silica nanoparticles; stencil printing
PMID: 29137115 DOI: 10.3390/s17112597
Free full text