tetano
Editor, Senior Moderator
Microbes Infect. 2010 Oct 22. [Epub ahead of print]
Correlation Between Sialic Acid Expression and Infection of Murine Macrophages by Different Strains of Influenza Virus.
Tate MD, Brooks AG, Reading PC.
Department of Microbiology and Immunology, University of Melbourne, Victoria, 3010, Australia.
Abstract
The mouse-adapted A/PR/8/34 (PR8; H1N1) virus infects airway macrophages poorly and is virulent in mice. Herein, we have investigated factors contributing to the ability of PR8 to evade murine macrophages. We demonstrate that the hemagglutinin of PR8 binds preferentially to α(2,3)-linked sialic acid (SA) and that murine macrophages express α(2,6)-linked SA. Moreover, resialylation of macrophages to express α(2,3)-linked SA restored susceptibility to PR8. Thus, during adaptation of human influenza viruses to growth in mice, a switch in receptor specificity from α(2,6)-linked SA to α(2,3)-linked SA is likely to favour evasion of attachment, entry and destruction by airway MΦ.
Copyright ? 2010. Published by Elsevier SAS.
PMID: 20974275 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20974275
Correlation Between Sialic Acid Expression and Infection of Murine Macrophages by Different Strains of Influenza Virus.
Tate MD, Brooks AG, Reading PC.
Department of Microbiology and Immunology, University of Melbourne, Victoria, 3010, Australia.
Abstract
The mouse-adapted A/PR/8/34 (PR8; H1N1) virus infects airway macrophages poorly and is virulent in mice. Herein, we have investigated factors contributing to the ability of PR8 to evade murine macrophages. We demonstrate that the hemagglutinin of PR8 binds preferentially to α(2,3)-linked sialic acid (SA) and that murine macrophages express α(2,6)-linked SA. Moreover, resialylation of macrophages to express α(2,3)-linked SA restored susceptibility to PR8. Thus, during adaptation of human influenza viruses to growth in mice, a switch in receptor specificity from α(2,6)-linked SA to α(2,3)-linked SA is likely to favour evasion of attachment, entry and destruction by airway MΦ.
Copyright ? 2010. Published by Elsevier SAS.
PMID: 20974275 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20974275