Mary Wilson
Well-known member
Published: 05 September 2022
DOI https://doi.org/10.1038/s41598-022-19222-4
Claudia Blaurock, Angele Breithaupt, Saskia Weber, Claudia Wylezich, Markus Keller, Björn-Patrick Mohl, Dirk Görlich, Martin H. Groschup, Balal Sadeghi, Dirk Höper, Thomas C. Mettenleiter & Anne Balkema-Buschmann
Abstract
Golden Syrian hamsters (Mesocricetus auratus) are used as a research model for severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Millions of Golden Syrian hamsters are also kept as pets in close contact to humans. To determine the minimum infective dose (MID) for assessing the zoonotic transmission risk, and to define the optimal infection dose for experimental studies, we orotracheally inoculated hamsters with SARS-CoV-2 doses from 1 * 10[SUP]5[/SUP] to 1 * 10[SUP]−4[/SUP] tissue culture infectious dose 50 (TCID[SUB]50[/SUB]). Body weight and virus shedding were monitored daily. 1 * 10[SUP]−3[/SUP] TCID[SUB]50[/SUB]was defined as the MID, and this was still sufficient to induce virus shedding at levels up to 10[SUP]2.75[/SUP] TCID[SUB]50[/SUB]/ml, equaling the estimated MID for humans. Virological and histological data revealed 1 * 10[SUP]2[/SUP]TCID[SUB]50[/SUB] as the optimal dose for experimental infections. This compelling high susceptibility leading to productive infections in Golden Syrian hamsters must be considered as a potential source of SARS-CoV-2 infection for humans that come into close contact with pet hamsters.
https://www.nature.com/articles/s41598-022-19222-4#citeas
DOI https://doi.org/10.1038/s41598-022-19222-4
Claudia Blaurock, Angele Breithaupt, Saskia Weber, Claudia Wylezich, Markus Keller, Björn-Patrick Mohl, Dirk Görlich, Martin H. Groschup, Balal Sadeghi, Dirk Höper, Thomas C. Mettenleiter & Anne Balkema-Buschmann
Abstract
Golden Syrian hamsters (Mesocricetus auratus) are used as a research model for severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Millions of Golden Syrian hamsters are also kept as pets in close contact to humans. To determine the minimum infective dose (MID) for assessing the zoonotic transmission risk, and to define the optimal infection dose for experimental studies, we orotracheally inoculated hamsters with SARS-CoV-2 doses from 1 * 10[SUP]5[/SUP] to 1 * 10[SUP]−4[/SUP] tissue culture infectious dose 50 (TCID[SUB]50[/SUB]). Body weight and virus shedding were monitored daily. 1 * 10[SUP]−3[/SUP] TCID[SUB]50[/SUB]was defined as the MID, and this was still sufficient to induce virus shedding at levels up to 10[SUP]2.75[/SUP] TCID[SUB]50[/SUB]/ml, equaling the estimated MID for humans. Virological and histological data revealed 1 * 10[SUP]2[/SUP]TCID[SUB]50[/SUB] as the optimal dose for experimental infections. This compelling high susceptibility leading to productive infections in Golden Syrian hamsters must be considered as a potential source of SARS-CoV-2 infection for humans that come into close contact with pet hamsters.
https://www.nature.com/articles/s41598-022-19222-4#citeas