tetano
Editor, Senior Moderator
J Clin Microbiol. 2013 Jul 3. [Epub ahead of print]
Comparison of the Filmarray RP, the Verigene RV+ and the Prodesse ProFLU+/FAST+ multiplex platforms for detection of influenza viruses in clinical samples from the 2011-2012 influenza season in Belgium.
Van Wesenbeeck L, Meeuws H, Van Immerseel A, Ispas G, Schmidt K, Houspie L, Van Ranst M, Stuyver L.
Source
Janssen Infectious Diseases - Diagnostics BVBA, Beerse, Belgium.
Abstract
Respiratory tract infections (RTI) are caused by a plethora of viral and bacterial pathogens. Especially lower RTI are a leading cause of hospitalization and mortality. Timely detection of the infecting respiratory pathogens is crucial to optimize treatment and care. In this study, FDA approved molecular multiplex platforms (Prodesse ProFLU+/FAST+, Filmarray RP and Verigene RV+) were evaluated for influenza virus detection in 171 clinical samples collected during the Belgian 2011-2012 influenza season. Sampling was done using mid-turbinate flocked swabs and the collected samples were stored in universal transport medium.The amount of viral RNA present in the swab samples ranged between 3.07 and 8.82 log10 copies/ml. Sixty samples were concordant influenza A positive and 8 samples were found to be concordant influenza B positive. Other respiratory viruses that were detected include human rhinovirus/enterovirus, respiratory syncytial virus, parainfluenza virus type 1, human metapneumovirus and coronavirus NL63. Twenty five samples had discordant results across the various assays which required further characterization by sequencing. In our hands, the Filmarray RP and the ProFLU+/FAST+ assays are convenient to work with regarding the sensitivity, ease of use and the low percentage of invalid results. Though the limit of sensitivity is of utmost importance, many other factors have to be taken into account for selecting the most convenient molecular-based diagnostic assay for the detection of respiratory pathogens in clinical samples.
PMID:
23824777
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23824777
Comparison of the Filmarray RP, the Verigene RV+ and the Prodesse ProFLU+/FAST+ multiplex platforms for detection of influenza viruses in clinical samples from the 2011-2012 influenza season in Belgium.
Van Wesenbeeck L, Meeuws H, Van Immerseel A, Ispas G, Schmidt K, Houspie L, Van Ranst M, Stuyver L.
Source
Janssen Infectious Diseases - Diagnostics BVBA, Beerse, Belgium.
Abstract
Respiratory tract infections (RTI) are caused by a plethora of viral and bacterial pathogens. Especially lower RTI are a leading cause of hospitalization and mortality. Timely detection of the infecting respiratory pathogens is crucial to optimize treatment and care. In this study, FDA approved molecular multiplex platforms (Prodesse ProFLU+/FAST+, Filmarray RP and Verigene RV+) were evaluated for influenza virus detection in 171 clinical samples collected during the Belgian 2011-2012 influenza season. Sampling was done using mid-turbinate flocked swabs and the collected samples were stored in universal transport medium.The amount of viral RNA present in the swab samples ranged between 3.07 and 8.82 log10 copies/ml. Sixty samples were concordant influenza A positive and 8 samples were found to be concordant influenza B positive. Other respiratory viruses that were detected include human rhinovirus/enterovirus, respiratory syncytial virus, parainfluenza virus type 1, human metapneumovirus and coronavirus NL63. Twenty five samples had discordant results across the various assays which required further characterization by sequencing. In our hands, the Filmarray RP and the ProFLU+/FAST+ assays are convenient to work with regarding the sensitivity, ease of use and the low percentage of invalid results. Though the limit of sensitivity is of utmost importance, many other factors have to be taken into account for selecting the most convenient molecular-based diagnostic assay for the detection of respiratory pathogens in clinical samples.
PMID:
23824777
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23824777