tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2018 Jul 18. doi: 10.1111/irv.12591. [Epub ahead of print]
[h=1]Comparison of haemagglutination inhibition, Single Radial Haemolysis, virus neutralization and ELISA assays TO detect antibody levels against seasonal influenza viruses.[/h] Trombetta CM[SUP]1[/SUP], Remarque EJ[SUP]2[/SUP], Mortier D[SUP]2[/SUP], Montomoli E[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The immunological response to influenza vaccine and/or natural infection is evaluated by serological techniques.,the most common being haemagglutination inhibition (HI), single radial haemolysis (SRH) and virus neutralization assays, which is commonly used in a Micro-Neutralization (MN) format. ELISA is not officially required, however this assay is able to measure different class-specific antibodies. The four assays identify different sets or subsets of antibodies.
[h=4]OBJECTIVES:[/h] The aim of this study was is to establish the correlation among four serological assays by using four seasonal influenza strains.
[h=4]METHODS:[/h] The HI, SRH, MN assays and ELISA were performed on four seasonal influenza strains.
[h=4]RESULTS:[/h] A strong positive correlation was found between HI and MN and between SRH and assays for influenza A strains. The B strains also showed good correlations among the three assays. A positive correlation is was also found between ELISA and the other "classical" assays for all the strains. Concerning the correlates of protection, as defined by HI ≥ 40 and SRH ≥ 25mm2, a good agreement was observed for the influenza A strains. By contrast, the agreement for the B strains was is very low.
[h=4]CONCLUSIONS:[/h] There is a positive strong correlation among the four serological assays for both A and B strains, especially for the HI and MN assays. There is a good agreement on correlates of protection between HI and SRH assays for the A strains, but very low agreement for the B strains, suggesting higher sensitivity of SRH than HI assay in for detecting antibodies against the influenza B viruses. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] ELISA ; Correlates of protection; Haemagglutination Inhibition assay; Single Radial Haemolysis; Virus Neutralization assay
PMID: 30019448 DOI: 10.1111/irv.12591
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[h=1]Comparison of haemagglutination inhibition, Single Radial Haemolysis, virus neutralization and ELISA assays TO detect antibody levels against seasonal influenza viruses.[/h] Trombetta CM[SUP]1[/SUP], Remarque EJ[SUP]2[/SUP], Mortier D[SUP]2[/SUP], Montomoli E[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The immunological response to influenza vaccine and/or natural infection is evaluated by serological techniques.,the most common being haemagglutination inhibition (HI), single radial haemolysis (SRH) and virus neutralization assays, which is commonly used in a Micro-Neutralization (MN) format. ELISA is not officially required, however this assay is able to measure different class-specific antibodies. The four assays identify different sets or subsets of antibodies.
[h=4]OBJECTIVES:[/h] The aim of this study was is to establish the correlation among four serological assays by using four seasonal influenza strains.
[h=4]METHODS:[/h] The HI, SRH, MN assays and ELISA were performed on four seasonal influenza strains.
[h=4]RESULTS:[/h] A strong positive correlation was found between HI and MN and between SRH and assays for influenza A strains. The B strains also showed good correlations among the three assays. A positive correlation is was also found between ELISA and the other "classical" assays for all the strains. Concerning the correlates of protection, as defined by HI ≥ 40 and SRH ≥ 25mm2, a good agreement was observed for the influenza A strains. By contrast, the agreement for the B strains was is very low.
[h=4]CONCLUSIONS:[/h] There is a positive strong correlation among the four serological assays for both A and B strains, especially for the HI and MN assays. There is a good agreement on correlates of protection between HI and SRH assays for the A strains, but very low agreement for the B strains, suggesting higher sensitivity of SRH than HI assay in for detecting antibodies against the influenza B viruses. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] ELISA ; Correlates of protection; Haemagglutination Inhibition assay; Single Radial Haemolysis; Virus Neutralization assay
PMID: 30019448 DOI: 10.1111/irv.12591
Free full text