tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis. 2019 Jan 16. pii: S0732-8893(18)30448-6. doi: 10.1016/j.diagmicrobio.2019.01.007. [Epub ahead of print]
[h=1]Comparison of FTD? respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.[/h] Kenmoe S[SUP]1[/SUP], Tcharnenwa C[SUP]2[/SUP], Monamele GC[SUP]3[/SUP], Kengne CN[SUP]4[/SUP], Ripa MN[SUP]1[/SUP], Whitaker B[SUP]5[/SUP], Alroy KA[SUP]5[/SUP], Balajee SA[SUP]5[/SUP], Njouom R[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]INTRODUCTION:[/h] This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD?) Respiratory Pathogens 33 multiplex test.
[h=4]METHODS:[/h] A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD? Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses.
[h=4]RESULTS:[/h] The results showed that 179/217 (82.5%) samples were positive with the singleplex method and 183/217 (84.3%) with the FTD? Respiratory Pathogens 33 multiplex test. Excellent or satisfactory agreement was obtained for all viruses (k > 0.6) except Parainfluenzavirus 4 (k = 0.24) and influenza B (k = 0.51).
[h=4]CONCLUSION:[/h] Although the multiplex FTD kits were more expensive than the singleplex assay, the FTD kits yielded rapid results in a shorter timeframe, increasing efficiency of diagnosis.
Copyright ? 2019 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cameroon; Molecular diagnostic; Respiratory viruses
PMID: 30738690 DOI: 10.1016/j.diagmicrobio.2019.01.007
[h=1]Comparison of FTD? respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.[/h] Kenmoe S[SUP]1[/SUP], Tcharnenwa C[SUP]2[/SUP], Monamele GC[SUP]3[/SUP], Kengne CN[SUP]4[/SUP], Ripa MN[SUP]1[/SUP], Whitaker B[SUP]5[/SUP], Alroy KA[SUP]5[/SUP], Balajee SA[SUP]5[/SUP], Njouom R[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]INTRODUCTION:[/h] This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD?) Respiratory Pathogens 33 multiplex test.
[h=4]METHODS:[/h] A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD? Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses.
[h=4]RESULTS:[/h] The results showed that 179/217 (82.5%) samples were positive with the singleplex method and 183/217 (84.3%) with the FTD? Respiratory Pathogens 33 multiplex test. Excellent or satisfactory agreement was obtained for all viruses (k > 0.6) except Parainfluenzavirus 4 (k = 0.24) and influenza B (k = 0.51).
[h=4]CONCLUSION:[/h] Although the multiplex FTD kits were more expensive than the singleplex assay, the FTD kits yielded rapid results in a shorter timeframe, increasing efficiency of diagnosis.
Copyright ? 2019 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cameroon; Molecular diagnostic; Respiratory viruses
PMID: 30738690 DOI: 10.1016/j.diagmicrobio.2019.01.007