tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis. 2017 Dec 16. pii: S0732-8893(17)30406-6. doi: 10.1016/j.diagmicrobio.2017.12.010. [Epub ahead of print]
[h=1]Comparison of Cepheid? Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1.[/h] Rabaan AA[SUP]1[/SUP], Bazzi AM[SUP]2[/SUP], Alshaikh SA[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] To compare two influenza polymerase chain reaction (PCR) methods.
[h=4]METHODS:[/h] A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid? Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay.
[h=4]RESULTS:[/h] There was 92.12% overall agreement between the two methods. Specificity of the Cepheid? Xpert Flu was 95.8% for H1N1 and 94.4% for total influenza A. Cepheid? Xpert Flu sensitivity for influenza A was 100% for younger patients (0-19-year age group) but significantly lower both for older patients (68.2% for 60-79-year and 50% for ≥80-year age groups) and overall for males compared to females (72.6% and 94.0%, respectively).
[h=4]CONCLUSIONS:[/h] Specificity of the Cepheid? Xpert Flu test was high; however, sensitivity for total influenza A was lower particularly in males and older patients.
Copyright ? 2017 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cepheid? Xpert flu; Influenza A; Influenza A (H1N1) pdm09; Real-time PCR; Saudi Arabia
PMID: 29329754 DOI: 10.1016/j.diagmicrobio.2017.12.010
[h=1]Comparison of Cepheid? Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1.[/h] Rabaan AA[SUP]1[/SUP], Bazzi AM[SUP]2[/SUP], Alshaikh SA[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] To compare two influenza polymerase chain reaction (PCR) methods.
[h=4]METHODS:[/h] A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid? Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay.
[h=4]RESULTS:[/h] There was 92.12% overall agreement between the two methods. Specificity of the Cepheid? Xpert Flu was 95.8% for H1N1 and 94.4% for total influenza A. Cepheid? Xpert Flu sensitivity for influenza A was 100% for younger patients (0-19-year age group) but significantly lower both for older patients (68.2% for 60-79-year and 50% for ≥80-year age groups) and overall for males compared to females (72.6% and 94.0%, respectively).
[h=4]CONCLUSIONS:[/h] Specificity of the Cepheid? Xpert Flu test was high; however, sensitivity for total influenza A was lower particularly in males and older patients.
Copyright ? 2017 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Cepheid? Xpert flu; Influenza A; Influenza A (H1N1) pdm09; Real-time PCR; Saudi Arabia
PMID: 29329754 DOI: 10.1016/j.diagmicrobio.2017.12.010