tetano
Editor, Senior Moderator
ScientificWorldJournal. 2014;2014:620580. doi: 10.1155/2014/620580. Epub 2014 Nov 23.
[h=1]Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis.[/h] Sultankulova KT[SUP]1[/SUP], Chervyakova OV[SUP]1[/SUP], Kozhabergenov NS[SUP]1[/SUP], Shorayeva KA[SUP]1[/SUP], Strochkov VM[SUP]1[/SUP], Orynbayev MB[SUP]1[/SUP], Sandybayev NT[SUP]1[/SUP], Sansyzbay AR[SUP]1[/SUP], Vasin AV[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185 specimens from humans, animals, and birds. IAVchip DNA microarray demonstrates higher diagnostic effectiveness (99.45%) in early influenza A diagnosis as compared to the real-time PCR (98.38%) and RT-PCR (96.22%), thus showing its clear superiority. Diagnostic sensitivity of IAVchip DNA microarray (100%) exceeds the same of RT-PCR (95.95%) and real-time RT-PCR (97.96%) in the range of estimated confidence intervals. IAVchip DNA microarray and real-time RT-PCR displayed equal diagnostic specificity (98.85%), while diagnostic specificity of RT-PCR was 96.40%. IAVchip DNA microarray has an advantage over the other tests for influenza A diagnosis and virus identification as a more rapid method that allows performing simultaneous detection and subtyping of about tens of specimens within one experiment during 8-10 hours. The developed IAVchip DNA microarray is a general test tool that enables identifying simultaneously 16 hemagglutinin (HA) and 9 neuraminidase (NA) subtypes of influenza A virus and also to screen the influenza A viruses from humans, animals, and birds by M and NP genes.
PMID: 25548788 [PubMed - in process] Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25548788
[h=1]Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis.[/h] Sultankulova KT[SUP]1[/SUP], Chervyakova OV[SUP]1[/SUP], Kozhabergenov NS[SUP]1[/SUP], Shorayeva KA[SUP]1[/SUP], Strochkov VM[SUP]1[/SUP], Orynbayev MB[SUP]1[/SUP], Sandybayev NT[SUP]1[/SUP], Sansyzbay AR[SUP]1[/SUP], Vasin AV[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185 specimens from humans, animals, and birds. IAVchip DNA microarray demonstrates higher diagnostic effectiveness (99.45%) in early influenza A diagnosis as compared to the real-time PCR (98.38%) and RT-PCR (96.22%), thus showing its clear superiority. Diagnostic sensitivity of IAVchip DNA microarray (100%) exceeds the same of RT-PCR (95.95%) and real-time RT-PCR (97.96%) in the range of estimated confidence intervals. IAVchip DNA microarray and real-time RT-PCR displayed equal diagnostic specificity (98.85%), while diagnostic specificity of RT-PCR was 96.40%. IAVchip DNA microarray has an advantage over the other tests for influenza A diagnosis and virus identification as a more rapid method that allows performing simultaneous detection and subtyping of about tens of specimens within one experiment during 8-10 hours. The developed IAVchip DNA microarray is a general test tool that enables identifying simultaneously 16 hemagglutinin (HA) and 9 neuraminidase (NA) subtypes of influenza A virus and also to screen the influenza A viruses from humans, animals, and birds by M and NP genes.
PMID: 25548788 [PubMed - in process] Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25548788