tetano
Editor, Senior Moderator
Commun Biol
. 2023 Aug 28;6(1):879.
doi: 10.1038/s42003-023-05253-8. Spatial transcriptome atlas reveals pulmonary microstructure-specific COVID-19 gene signatures in cynomolgus macaques
Taehwan Oh[SUP] 1 [/SUP], Green Kim[SUP] 1 [/SUP], Seung Ho Baek[SUP] 1 [/SUP], YoungMin Woo[SUP] 1 2 [/SUP], Bon-Sang Koo[SUP] 1 [/SUP], Eun-Ha Hwang[SUP] 1 [/SUP], Kyuyoung Shim[SUP] 1 2 [/SUP], You Jung An[SUP] 1 [/SUP], Yujin Kim[SUP] 1 [/SUP], Jinyoung Won[SUP] 1 [/SUP], Youngjeon Lee[SUP] 1 [/SUP], Kyung Seob Lim[SUP] 3 [/SUP], Jae-Hak Park[SUP] 4 [/SUP], Jung Joo Hong[SUP] 5 6 [/SUP]
Affiliations
Characterizing the host response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at the molecular level is necessary to understand viral pathogenesis and identify clinically relevant biomarkers. However, in humans, the pulmonary host response during disease onset remains poorly understood. Herein, we utilized a spatial transcriptome atlas to identify pulmonary microstructure-specific COVID-19 gene signatures during the acute phase of lung infection in cynomolgus macaques. The innate immune response to virus-induced cell death was primarily active in the alveolar regions involving activated macrophage infiltration. Inflamed vascular regions exhibited prominent upregulation of interferon and complement pathway genes that mediate antiviral activity and tissue damage response. Furthermore, known biomarker genes were significantly expressed in specific microstructures, and some of them were universally expressed across all microstructures. These findings underscore the importance of identifying key drivers of disease progression and clinically applicable biomarkers by focusing on pulmonary microstructures appearing during SARS-CoV-2 infection.
. 2023 Aug 28;6(1):879.
doi: 10.1038/s42003-023-05253-8. Spatial transcriptome atlas reveals pulmonary microstructure-specific COVID-19 gene signatures in cynomolgus macaques
Taehwan Oh[SUP] 1 [/SUP], Green Kim[SUP] 1 [/SUP], Seung Ho Baek[SUP] 1 [/SUP], YoungMin Woo[SUP] 1 2 [/SUP], Bon-Sang Koo[SUP] 1 [/SUP], Eun-Ha Hwang[SUP] 1 [/SUP], Kyuyoung Shim[SUP] 1 2 [/SUP], You Jung An[SUP] 1 [/SUP], Yujin Kim[SUP] 1 [/SUP], Jinyoung Won[SUP] 1 [/SUP], Youngjeon Lee[SUP] 1 [/SUP], Kyung Seob Lim[SUP] 3 [/SUP], Jae-Hak Park[SUP] 4 [/SUP], Jung Joo Hong[SUP] 5 6 [/SUP]
Affiliations
- PMID: 37640792
- DOI: 10.1038/s42003-023-05253-8
Characterizing the host response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at the molecular level is necessary to understand viral pathogenesis and identify clinically relevant biomarkers. However, in humans, the pulmonary host response during disease onset remains poorly understood. Herein, we utilized a spatial transcriptome atlas to identify pulmonary microstructure-specific COVID-19 gene signatures during the acute phase of lung infection in cynomolgus macaques. The innate immune response to virus-induced cell death was primarily active in the alveolar regions involving activated macrophage infiltration. Inflamed vascular regions exhibited prominent upregulation of interferon and complement pathway genes that mediate antiviral activity and tissue damage response. Furthermore, known biomarker genes were significantly expressed in specific microstructures, and some of them were universally expressed across all microstructures. These findings underscore the importance of identifying key drivers of disease progression and clinically applicable biomarkers by focusing on pulmonary microstructures appearing during SARS-CoV-2 infection.