tetano
Editor, Senior Moderator
Commun Biol
. 2025 Jul 26;8(1):1108.
doi: 10.1038/s42003-025-08507-9. Single-cell antigen receptor sequencing in pigs with influenza
Weihong Gu[SUP] 1 2 [/SUP], Darling Melany de Carvahlo Madrid[SUP] 1 2 [/SUP], Yuhan Wen[SUP] 1 2 [/SUP], Sadie Clements[SUP] 1 2 [/SUP], Laurie Touchard[SUP] 1 2 [/SUP], Nathan Bivens[SUP] 3 [/SUP], Grant Zane[SUP] 3 [/SUP], Mingyi Zhou[SUP] 3 [/SUP], Kiho Lee[SUP] 1 [/SUP], John P Driver[SUP] 4 5 [/SUP]
Affiliations
Single-cell RNA sequencing (scRNAseq) has accelerated characterizing cellular phenotypes in pigs under healthy and diseased conditions. To pair scRNAseq with immune receptor profiling, we developed porcine-specific T cell receptor (TCR) and B cell receptor (BCR) enrichment primers that are compatible with the 10 × Genomics VDJ sequencing protocol. Using these assays, we profiled the immune repertoire of cryopreserved lung cells from CD1D-expressing and CD1D-deficient pigs after one or two infections with influenza A virus (IAV) to examine whether natural killer T (NKT) cells influence pulmonary TCR and BCR receptor repertoires. We also profiled T cells longitudinally sampled from the lung fluid of IAV-vaccinated and -infected pigs to track clonal expansion. While all pigs presented highly diverse repertoires, pigs re-exposed to IAV had more expanded T cell clonotypes with activated phenotypes, suggesting potential IAV-reactive clones. Our results demonstrate the utility of high throughput single cell TCR and BCR sequencing in pigs.
. 2025 Jul 26;8(1):1108.
doi: 10.1038/s42003-025-08507-9. Single-cell antigen receptor sequencing in pigs with influenza
Weihong Gu[SUP] 1 2 [/SUP], Darling Melany de Carvahlo Madrid[SUP] 1 2 [/SUP], Yuhan Wen[SUP] 1 2 [/SUP], Sadie Clements[SUP] 1 2 [/SUP], Laurie Touchard[SUP] 1 2 [/SUP], Nathan Bivens[SUP] 3 [/SUP], Grant Zane[SUP] 3 [/SUP], Mingyi Zhou[SUP] 3 [/SUP], Kiho Lee[SUP] 1 [/SUP], John P Driver[SUP] 4 5 [/SUP]
Affiliations
- PMID: 40715353
- PMCID: PMC12297343
- DOI: 10.1038/s42003-025-08507-9
Single-cell RNA sequencing (scRNAseq) has accelerated characterizing cellular phenotypes in pigs under healthy and diseased conditions. To pair scRNAseq with immune receptor profiling, we developed porcine-specific T cell receptor (TCR) and B cell receptor (BCR) enrichment primers that are compatible with the 10 × Genomics VDJ sequencing protocol. Using these assays, we profiled the immune repertoire of cryopreserved lung cells from CD1D-expressing and CD1D-deficient pigs after one or two infections with influenza A virus (IAV) to examine whether natural killer T (NKT) cells influence pulmonary TCR and BCR receptor repertoires. We also profiled T cells longitudinally sampled from the lung fluid of IAV-vaccinated and -infected pigs to track clonal expansion. While all pigs presented highly diverse repertoires, pigs re-exposed to IAV had more expanded T cell clonotypes with activated phenotypes, suggesting potential IAV-reactive clones. Our results demonstrate the utility of high throughput single cell TCR and BCR sequencing in pigs.