tetano
Editor, Senior Moderator
First published November 2011, doi: 10.1128/JVI.05699-11 J. Virol. January 2012 vol. 86 no. 2 1233-1237
Combination of PB2 271A and SR Polymorphism at Positions 590/591 Is Critical for Viral Replication and Virulence of Swine Influenza Virus in Cultured Cells and In Vivo
Qinfang Liua,
Chuanling Qiaoa,*,
Henju Marjukib,
Bhupinder Bawaa,
Jingqun Maa,
Stephane Guillossoua,
Richard J. Webbyb,
J?rgen A. Richta and
Wenjun Maa
+ Author Affiliations
aDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
bSt. Jude Children's Research Hospital, Memphis, Tennessee, USA
ABSTRACT
Triple reassortant swine influenza viruses (SIVs) and 2009 pandemic H1N1 (pH1N1) virus contain an avian-origin PB2 with 271A, 590S, 591R, and 627E. To evaluate the role of PB2 271A, 590S, and 591R in the replication and virulence of SIV, single (1930-TX98-PB2-271T)-, double (1930-TX98-PB2-590A591A)-, and triple (1930-TX98-PB2-271T590A591A)-mutated viruses were generated in the background of the H1N1 A/swine/Iowa/15/30 (1930) virus with an avian-origin PB2 from the triple-reassortant A/swine/Texas/4199-2/98 (TX98) virus, called the parental 1930-TX98-PB2. Compared to parental virus and single- and double-mutated viruses, the triple-mutated virus replicated less efficiently in cell cultures and was attenuated in mice. These results suggest that a combination of 271A with the 590/591 SR polymorphism is critical for pH1N1 and triple-reassortant SIVs for efficient replication and adaptation in mammals.
http://jvi.asm.org/content/86/2/1233.short?rss=1
Combination of PB2 271A and SR Polymorphism at Positions 590/591 Is Critical for Viral Replication and Virulence of Swine Influenza Virus in Cultured Cells and In Vivo
Qinfang Liua,
Chuanling Qiaoa,*,
Henju Marjukib,
Bhupinder Bawaa,
Jingqun Maa,
Stephane Guillossoua,
Richard J. Webbyb,
J?rgen A. Richta and
Wenjun Maa
+ Author Affiliations
aDepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA
bSt. Jude Children's Research Hospital, Memphis, Tennessee, USA
ABSTRACT
Triple reassortant swine influenza viruses (SIVs) and 2009 pandemic H1N1 (pH1N1) virus contain an avian-origin PB2 with 271A, 590S, 591R, and 627E. To evaluate the role of PB2 271A, 590S, and 591R in the replication and virulence of SIV, single (1930-TX98-PB2-271T)-, double (1930-TX98-PB2-590A591A)-, and triple (1930-TX98-PB2-271T590A591A)-mutated viruses were generated in the background of the H1N1 A/swine/Iowa/15/30 (1930) virus with an avian-origin PB2 from the triple-reassortant A/swine/Texas/4199-2/98 (TX98) virus, called the parental 1930-TX98-PB2. Compared to parental virus and single- and double-mutated viruses, the triple-mutated virus replicated less efficiently in cell cultures and was attenuated in mice. These results suggest that a combination of 271A with the 590/591 SR polymorphism is critical for pH1N1 and triple-reassortant SIVs for efficient replication and adaptation in mammals.
http://jvi.asm.org/content/86/2/1233.short?rss=1