tetano
Editor, Senior Moderator
J Virol. 2011 Nov 9. [Epub ahead of print]
Combination of PB2 271A and SR polymorphism at positions 590/591 is critical for viral replication and virulence of swine influenza virus in cultured cells and in vivo.
Liu Q, Qiao C, Marjuki H, Bawa B, Ma J, Guillossou S, Webby RJ, Richt JA, Ma W.
Source
Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, USA.
Abstract
Triple reassortant swine influenza viruses (SIVs) and 2009 pandemic H1N1 (pH1N1) virus contain an avian-origin PB2 with 271A, 590S, 591R and 627E. To evaluate the role of PB2 271A, 590S and 591R in replication and virulence of SIV, single- (1930-TX98-PB2-271T), double- (1930-TX98-PB2-590A591A) and triple-mutated (1930-TX98-PB2-271T590A591A) viruses were generated in the background of the H1N1 A/swine/Iowa/15/30 (1930) virus with an avian-origin PB2 from the triple reassortant A/swine/Texas/4199-2/98 (TX98) virus, called the parental 1930-TX98-PB2. Compared to parental, single- and double-mutated viruses, the triple-mutated virus replicated less efficiently in cell cultures and was attenuated in mice. These results suggest that a combination of 271A with 590/591 SR polymorphism is critical for pH1N1 and triple reassortant SIVs for efficient replication and adaptation in mammals.
PMID:
22072752
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22072752
Combination of PB2 271A and SR polymorphism at positions 590/591 is critical for viral replication and virulence of swine influenza virus in cultured cells and in vivo.
Liu Q, Qiao C, Marjuki H, Bawa B, Ma J, Guillossou S, Webby RJ, Richt JA, Ma W.
Source
Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, USA.
Abstract
Triple reassortant swine influenza viruses (SIVs) and 2009 pandemic H1N1 (pH1N1) virus contain an avian-origin PB2 with 271A, 590S, 591R and 627E. To evaluate the role of PB2 271A, 590S and 591R in replication and virulence of SIV, single- (1930-TX98-PB2-271T), double- (1930-TX98-PB2-590A591A) and triple-mutated (1930-TX98-PB2-271T590A591A) viruses were generated in the background of the H1N1 A/swine/Iowa/15/30 (1930) virus with an avian-origin PB2 from the triple reassortant A/swine/Texas/4199-2/98 (TX98) virus, called the parental 1930-TX98-PB2. Compared to parental, single- and double-mutated viruses, the triple-mutated virus replicated less efficiently in cell cultures and was attenuated in mice. These results suggest that a combination of 271A with 590/591 SR polymorphism is critical for pH1N1 and triple reassortant SIVs for efficient replication and adaptation in mammals.
PMID:
22072752
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22072752