tetano
Editor, Senior Moderator
Vaccine. 2014 Mar 11. pii: S0264-410X(14)00306-5. doi: 10.1016/j.vaccine.2014.02.079. [Epub ahead of print]
Cold adaptation improves the growth of seasonal influenza B vaccine viruses.
Kim H1, Schoofs P2, Anderson DA3, Tannock GA4, Rockman SP2.
Author information
Abstract
Gene reassortment has proved useful in improving yields of influenza A antigens of egg-based inactivated vaccines, but similar approaches have been difficult with influenza B antigens. Current regulations for influenza vaccine seed viruses limit the number of egg passages and as a result resultant yields from influenza B vaccine seed viruses are frequently inconsistent. Therefore, reliable approaches to enhance yields of influenza B vaccine seed viruses are required for efficient vaccine manufacture. In the present study three stable cold-adapted (ca) mutants, caF, caM and caB derived from seasonal epidemic strains, B/Florida/4/2006, B/Malaysia/2506/2004 and B/Brisbane/60/2008 were prepared, which produced high hemagglutinin antigen yields and also increased viral yields of reassortants possessing the desired 6:2 gene constellation. The results demonstrate that consistent improvements in yields of influenza B viruses can be obtained by cold adaptation following extended passage. Taken together, the three ca viruses were shown to have potential as donor viruses for the preparation of high-yielding influenza B vaccine viruses by reassortment.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Cold adaptation, Growth, Inactivated vaccine, Influenza B virus
PMID:
24631096
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24631096
Cold adaptation improves the growth of seasonal influenza B vaccine viruses.
Kim H1, Schoofs P2, Anderson DA3, Tannock GA4, Rockman SP2.
Author information
Abstract
Gene reassortment has proved useful in improving yields of influenza A antigens of egg-based inactivated vaccines, but similar approaches have been difficult with influenza B antigens. Current regulations for influenza vaccine seed viruses limit the number of egg passages and as a result resultant yields from influenza B vaccine seed viruses are frequently inconsistent. Therefore, reliable approaches to enhance yields of influenza B vaccine seed viruses are required for efficient vaccine manufacture. In the present study three stable cold-adapted (ca) mutants, caF, caM and caB derived from seasonal epidemic strains, B/Florida/4/2006, B/Malaysia/2506/2004 and B/Brisbane/60/2008 were prepared, which produced high hemagglutinin antigen yields and also increased viral yields of reassortants possessing the desired 6:2 gene constellation. The results demonstrate that consistent improvements in yields of influenza B viruses can be obtained by cold adaptation following extended passage. Taken together, the three ca viruses were shown to have potential as donor viruses for the preparation of high-yielding influenza B vaccine viruses by reassortment.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Cold adaptation, Growth, Inactivated vaccine, Influenza B virus
PMID:
24631096
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24631096