tetano
Editor, Senior Moderator
Virology. 2015 Jun 23;484:203-212. doi: 10.1016/j.virol.2015.06.002. [Epub ahead of print]
[h=1]Co-infection of influenza A viruses of swine contributes to effective shuffling of gene segments in a naturally reared pig.[/h] Abe H[SUP]1[/SUP], Mine J[SUP]1[/SUP], Parchariyanon S[SUP]2[/SUP], Takemae N[SUP]1[/SUP], Boonpornprasert P[SUP]2[/SUP], Ubonyaem N[SUP]2[/SUP], Patcharasinghawut P[SUP]2[/SUP], Nuansrichay B[SUP]2[/SUP], Tanikawa T[SUP]1[/SUP], Tsunekuni R[SUP]1[/SUP], Saito T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Following the 2009 H1N1 pandemic, surveillance activities have been accelerated globally to monitor the emergence of novel reassortant viruses. However, the mechanism by which influenza A viruses of swine (IAV-S) acquire novel gene constellations through reassortment events in natural settings remains poorly understood. To explore the mechanism, we collected 785 nasal swabs from pigs in a farm in Thailand from 2011 to 2014. H3N2, H3N1, H1N1 and H1N2 IAVs-S were isolated from a single co-infected sample by plaque purification and showed a high degree of diversity of the genome. In particular, the H1N1 isolates, possessing a novel gene constellation previously unreported in Thailand, exhibited greater variation in internal genes than H3N2 IAVs-S. A pair of isolates, designated H3N2-B and H1N1-D, was determined to have been initially introduced to the farm. These results demonstrate that numerous IAVs-S with various gene constellations can be created in a single co-infected pig via reassortment.
Copyright ? 2015 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Bayesian MCMC; Co-infection; IAV-S; Surveillance; Swine influenza virus; Thailand
PMID: 26115167 [PubMed - as supplied by publisher]
[h=1]Co-infection of influenza A viruses of swine contributes to effective shuffling of gene segments in a naturally reared pig.[/h] Abe H[SUP]1[/SUP], Mine J[SUP]1[/SUP], Parchariyanon S[SUP]2[/SUP], Takemae N[SUP]1[/SUP], Boonpornprasert P[SUP]2[/SUP], Ubonyaem N[SUP]2[/SUP], Patcharasinghawut P[SUP]2[/SUP], Nuansrichay B[SUP]2[/SUP], Tanikawa T[SUP]1[/SUP], Tsunekuni R[SUP]1[/SUP], Saito T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Following the 2009 H1N1 pandemic, surveillance activities have been accelerated globally to monitor the emergence of novel reassortant viruses. However, the mechanism by which influenza A viruses of swine (IAV-S) acquire novel gene constellations through reassortment events in natural settings remains poorly understood. To explore the mechanism, we collected 785 nasal swabs from pigs in a farm in Thailand from 2011 to 2014. H3N2, H3N1, H1N1 and H1N2 IAVs-S were isolated from a single co-infected sample by plaque purification and showed a high degree of diversity of the genome. In particular, the H1N1 isolates, possessing a novel gene constellation previously unreported in Thailand, exhibited greater variation in internal genes than H3N2 IAVs-S. A pair of isolates, designated H3N2-B and H1N1-D, was determined to have been initially introduced to the farm. These results demonstrate that numerous IAVs-S with various gene constellations can be created in a single co-infected pig via reassortment.
Copyright ? 2015 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Bayesian MCMC; Co-infection; IAV-S; Surveillance; Swine influenza virus; Thailand
PMID: 26115167 [PubMed - as supplied by publisher]