tetano
Editor, Senior Moderator
Clinics (Sao Paulo)
. 2021 Dec 6;76:e3548.
doi: 10.6061/clinics/2021/e3548. eCollection 2021.
SARS-CoV-2 recombinant proteins stimulate distinct cellular and humoral immune response profiles in samples from COVID-19 convalescent patients
Laís Teodoro da Silva[SUP] 1 [/SUP], Marina Mazzilli Ortega[SUP] 1 [/SUP], Bruna Tiaki Tiyo[SUP] 1 [/SUP], Isabelle Freire Tabosa Viana[SUP] 2 [/SUP], Tayná Evily de Lima[SUP] 2 [/SUP], Tania Regina Tozetto-Mendoza[SUP] 3 [/SUP], Luanda Mara da Silva Oliveira[SUP] 1 [/SUP], Franciane Mouradian Emidio Teixeira[SUP] 1 [/SUP], Roberto Dias Lins[SUP] 2 [/SUP], Alexandre de Almeida[SUP] 1 [/SUP], Maria Cassia Mendes-Correa[SUP] 3 [/SUP], Alberto Jose da Silva Duarte[SUP] 1 4 [/SUP], Telma Miyuki Oshiro[SUP] 1 [/SUP]
Affiliations
Abstract
Objectives: In this preliminary study we investigated cellular and humoral immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in blood samples from 14 recovered coronavirus disease 2019 (COVID-19) patients and compared them to those in samples from 12 uninfected/unvaccinated volunteers.
Methods: Cellular immunity was assessed by intracellular detection of IFN-γ in CD3+ T lymphocytes after stimulation with SARS-CoV-2 spike (S1), nucleocapsid (NC), or receptor-binding domain (RBD) recombinant proteins or overlapping peptide pools covering the sequence of SARS-CoV-2 spike, membrane and nucleocapsid regions. The humoral response was examined by ELISAs and/or chemiluminescence assays for the presence of serum IgG antibodies directed to SARS-CoV-2 proteins.
Results: We observed differences between humoral and cellular immune profiles in response to stimulation with the same proteins. Assays of IgG antibodies directed to SARS-CoV-2 NC, RBD and S1/S2 recombinant proteins were able to differentiate convalescent from uninfected/unvaccinated groups. Cellular immune responses to SARS-CoV-2 protein stimuli did not exhibit a specific response, as T cells from both individuals with no history of contact with SARS-CoV-2 and from recovered donors were able to produce IFN-γ.
Conclusions: Determination of the cellular immune response to stimulation with a pool of SARS-CoV-2 peptides but not with SARS-CoV-2 proteins is able to distinguish convalescent individuals from unexposed individuals. Regarding the humoral immune response, the screening for serum IgG antibodies directed to SARS-CoV-2 proteins has been shown to be specific for the response of recovered individuals.
. 2021 Dec 6;76:e3548.
doi: 10.6061/clinics/2021/e3548. eCollection 2021.
SARS-CoV-2 recombinant proteins stimulate distinct cellular and humoral immune response profiles in samples from COVID-19 convalescent patients
Laís Teodoro da Silva[SUP] 1 [/SUP], Marina Mazzilli Ortega[SUP] 1 [/SUP], Bruna Tiaki Tiyo[SUP] 1 [/SUP], Isabelle Freire Tabosa Viana[SUP] 2 [/SUP], Tayná Evily de Lima[SUP] 2 [/SUP], Tania Regina Tozetto-Mendoza[SUP] 3 [/SUP], Luanda Mara da Silva Oliveira[SUP] 1 [/SUP], Franciane Mouradian Emidio Teixeira[SUP] 1 [/SUP], Roberto Dias Lins[SUP] 2 [/SUP], Alexandre de Almeida[SUP] 1 [/SUP], Maria Cassia Mendes-Correa[SUP] 3 [/SUP], Alberto Jose da Silva Duarte[SUP] 1 4 [/SUP], Telma Miyuki Oshiro[SUP] 1 [/SUP]
Affiliations
- PMID: 34878034
- DOI: 10.6061/clinics/2021/e3548
Abstract
Objectives: In this preliminary study we investigated cellular and humoral immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in blood samples from 14 recovered coronavirus disease 2019 (COVID-19) patients and compared them to those in samples from 12 uninfected/unvaccinated volunteers.
Methods: Cellular immunity was assessed by intracellular detection of IFN-γ in CD3+ T lymphocytes after stimulation with SARS-CoV-2 spike (S1), nucleocapsid (NC), or receptor-binding domain (RBD) recombinant proteins or overlapping peptide pools covering the sequence of SARS-CoV-2 spike, membrane and nucleocapsid regions. The humoral response was examined by ELISAs and/or chemiluminescence assays for the presence of serum IgG antibodies directed to SARS-CoV-2 proteins.
Results: We observed differences between humoral and cellular immune profiles in response to stimulation with the same proteins. Assays of IgG antibodies directed to SARS-CoV-2 NC, RBD and S1/S2 recombinant proteins were able to differentiate convalescent from uninfected/unvaccinated groups. Cellular immune responses to SARS-CoV-2 protein stimuli did not exhibit a specific response, as T cells from both individuals with no history of contact with SARS-CoV-2 and from recovered donors were able to produce IFN-γ.
Conclusions: Determination of the cellular immune response to stimulation with a pool of SARS-CoV-2 peptides but not with SARS-CoV-2 proteins is able to distinguish convalescent individuals from unexposed individuals. Regarding the humoral immune response, the screening for serum IgG antibodies directed to SARS-CoV-2 proteins has been shown to be specific for the response of recovered individuals.