Clinical utility comparison of two benchtop deep sequencing instruments for rapid diagnosis of newly emergent influenza infections

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Clin Microbiol Infect. 2014 Oct 29. pii: S1198-743X(14)00078-0. doi: 10.1016/j.cmi.2014.10.016. [Epub ahead of print]
[h=1]Clinical utility comparison of two benchtop deep sequencing instruments for rapid diagnosis of newly emergent influenza infections.[/h] Ren X[SUP]1[/SUP], Hu Y[SUP]1[/SUP], Yang F[SUP]1[/SUP], Xiao Y[SUP]1[/SUP], Li L[SUP]1[/SUP], Dong J[SUP]1[/SUP], Sun L[SUP]1[/SUP], Zhu Y[SUP]1[/SUP], Jin Q[SUP]2[/SUP].
[h=3]Author information[/h]

[h=3]Abstract[/h] Rapid determination of full genomes of influenza A viruses from clinical samples is vital to outbreak preparedness. We benchmarked Ion Torrent PGM and Illumina MiSeq to the standard Sanger sequencing on two real clinical samples and demonstrated their performance for full-genome determination and polymorphism identification.
Copyright ? 2014 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.


[h=4]KEYWORDS:[/h] Deep sequencing; Ion Torrent PGM; MiSeq; genome sequencing; influenza

PMID: 25658538 [PubMed - as supplied by publisher]