tetano
Editor, Senior Moderator
Med Princ Pract. 2019 Mar 4. doi: 10.1159/000499313. [Epub ahead of print]
[h=1]Clinical Performance of Allplex Respiratory Panel 1 Test Compared to Simplexa Flu A/B and RSV for the Detection of Influenza Virus and Respiratory Syncytial Virus Infection Including Their Subtyping.[/h] Kim J, Nam J, Jang W, Lim CS.
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] The Allplex Respiratory Panel 1 (ARP) is a new assay based on a real-time polymerase chain reaction (RT-PCR) for the detection of influenza A (Flu A), influenza B virus (Flu B) and respiratory syncytial virus (RSV), including subtyping by multiple detection temperature (MuDT) technology. We evaluated the performance of the Allplex Respiratory Panel compared to SimplexaTM Flu A/B & RSV assay (SP) and other diagnostic tools.
[h=4]MATERIALS AND METHODS:[/h] A total of 372 samples were collected from patients at the Korea University Guro Hospital in Seoul, Korea. All samples were tested for influenza virus and RSV by ARP, SP, and an in-house RT-PCR.
[h=4]RESULTS:[/h] Sensitivity of ARP was 95.56%, 100%, and 95.24% for Flu A, Flu B, and RSV, respectively. Specificity of ARP was 100%, 100%, and 100% for Flu A, Flu B, and RSV, respectively. SP had sensitivities and specificities of 98.89% and 100% for Flu A, 100% and 100% for Flu B, and 100% and 100% for RSV.
[h=4]CONCLUSION:[/h] The Allplex panel showed high sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for the detection of Flu A, Flu B, and RSV. This assay is fast and easy to perform because it takes only about 150 minutes, and there is no need for post-PCR electrophoresis. The ARP can be used as a reliable and convenient assay in clinical laboratories.
?2019The Author(s). Published by S. Karger AG, Basel.
PMID: 30831570 DOI: 10.1159/000499313
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[h=1]Clinical Performance of Allplex Respiratory Panel 1 Test Compared to Simplexa Flu A/B and RSV for the Detection of Influenza Virus and Respiratory Syncytial Virus Infection Including Their Subtyping.[/h] Kim J, Nam J, Jang W, Lim CS.
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] The Allplex Respiratory Panel 1 (ARP) is a new assay based on a real-time polymerase chain reaction (RT-PCR) for the detection of influenza A (Flu A), influenza B virus (Flu B) and respiratory syncytial virus (RSV), including subtyping by multiple detection temperature (MuDT) technology. We evaluated the performance of the Allplex Respiratory Panel compared to SimplexaTM Flu A/B & RSV assay (SP) and other diagnostic tools.
[h=4]MATERIALS AND METHODS:[/h] A total of 372 samples were collected from patients at the Korea University Guro Hospital in Seoul, Korea. All samples were tested for influenza virus and RSV by ARP, SP, and an in-house RT-PCR.
[h=4]RESULTS:[/h] Sensitivity of ARP was 95.56%, 100%, and 95.24% for Flu A, Flu B, and RSV, respectively. Specificity of ARP was 100%, 100%, and 100% for Flu A, Flu B, and RSV, respectively. SP had sensitivities and specificities of 98.89% and 100% for Flu A, 100% and 100% for Flu B, and 100% and 100% for RSV.
[h=4]CONCLUSION:[/h] The Allplex panel showed high sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for the detection of Flu A, Flu B, and RSV. This assay is fast and easy to perform because it takes only about 150 minutes, and there is no need for post-PCR electrophoresis. The ARP can be used as a reliable and convenient assay in clinical laboratories.
?2019The Author(s). Published by S. Karger AG, Basel.
PMID: 30831570 DOI: 10.1159/000499313
Free full text