tetano
Editor, Senior Moderator
Clin Transl Immunology
. 2020 Dec 9;9(12):e1227.
doi: 10.1002/cti2.1227. eCollection 2020.
Identification of similar epitopes between severe acute respiratory syndrome coronavirus-2 and Bacillus Calmette-Gu?rin: potential for cross-reactive adaptive immunity
Szabolcs Urb?n[SUP] 1 [/SUP], G?bor Paragi[SUP] 2 3 [/SUP], Katalin Buri?n[SUP] 4 [/SUP], Gary R McLean[SUP] 5 6 [/SUP], Dezső P Virok[SUP] 4 [/SUP]
Affiliations
Abstract
Objectives: Bacillus Calmette-Gu?rin (BCG) vaccination has been implicated in protection against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and as a non-specific immunisation method against the virus. We therefore decided to investigate T-cell and B-cell epitopes within the BCG-Pasteur strain proteome for similarity to immunogenic peptides of SARS-CoV-2.
Methods: We used NetMHC 4.0 and BepiPred 2.0 epitope prediction methods for the analysis of the BCG-Pasteur proteome to identify similar peptides to established and novel SARS-CoV-2 T-cell and B-cell epitopes.
Results: We found 112 BCG MHC-I-restricted T-cell epitopes similar to MHC-I-restricted T-cell SARS-CoV-2 epitopes and 690 BCG B-cell epitopes similar to SARS-CoV-2 B-cell epitopes. The SARS-CoV-2 T-cell epitopes represented 16 SARS-CoV-2 proteins, and the SARS-CoV-2 B-cell epitopes represented 5 SARS-CoV-2 proteins, including the receptor binding domain of the spike glycoprotein.
Conclusion: Altogether, our results provide a mechanistic basis for the potential cross-reactive adaptive immunity that may exist between the two microorganisms.
Keywords: 2019‐nCoV; BCG; Bacillus Calmette–Gu?rin; COVID‐19; SARS‐CoV‐2.
. 2020 Dec 9;9(12):e1227.
doi: 10.1002/cti2.1227. eCollection 2020.
Identification of similar epitopes between severe acute respiratory syndrome coronavirus-2 and Bacillus Calmette-Gu?rin: potential for cross-reactive adaptive immunity
Szabolcs Urb?n[SUP] 1 [/SUP], G?bor Paragi[SUP] 2 3 [/SUP], Katalin Buri?n[SUP] 4 [/SUP], Gary R McLean[SUP] 5 6 [/SUP], Dezső P Virok[SUP] 4 [/SUP]
Affiliations
- PMID: 33318797
- PMCID: PMC7724920
- DOI: 10.1002/cti2.1227
Abstract
Objectives: Bacillus Calmette-Gu?rin (BCG) vaccination has been implicated in protection against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and as a non-specific immunisation method against the virus. We therefore decided to investigate T-cell and B-cell epitopes within the BCG-Pasteur strain proteome for similarity to immunogenic peptides of SARS-CoV-2.
Methods: We used NetMHC 4.0 and BepiPred 2.0 epitope prediction methods for the analysis of the BCG-Pasteur proteome to identify similar peptides to established and novel SARS-CoV-2 T-cell and B-cell epitopes.
Results: We found 112 BCG MHC-I-restricted T-cell epitopes similar to MHC-I-restricted T-cell SARS-CoV-2 epitopes and 690 BCG B-cell epitopes similar to SARS-CoV-2 B-cell epitopes. The SARS-CoV-2 T-cell epitopes represented 16 SARS-CoV-2 proteins, and the SARS-CoV-2 B-cell epitopes represented 5 SARS-CoV-2 proteins, including the receptor binding domain of the spike glycoprotein.
Conclusion: Altogether, our results provide a mechanistic basis for the potential cross-reactive adaptive immunity that may exist between the two microorganisms.
Keywords: 2019‐nCoV; BCG; Bacillus Calmette–Gu?rin; COVID‐19; SARS‐CoV‐2.