tetano
Editor, Senior Moderator
Clin Proteomics
. 2024 May 22;21(1):37.
doi: 10.1186/s12014-024-09482-9. Proteomic snapshot of saliva samples predicts new pathways implicated in SARS-CoV-2 pathogenesis
Elena Moreno[SUP] #[/SUP][SUP] 1 2 [/SUP], Sergio Ciordia[SUP] #[/SUP][SUP] 3 [/SUP], Santos Milhano Fátima[SUP] #[/SUP][SUP] 3 [/SUP], Daniel Jiménez[SUP] 4 [/SUP], Javier Martínez-Sanz[SUP] 4 5 [/SUP], Pilar Vizcarra[SUP] 4 5 [/SUP], Raquel Ron[SUP] 4 5 [/SUP], Matilde Sánchez-Conde[SUP] 4 5 [/SUP], Rafael Bargiela[SUP] 6 [/SUP], Sergio Sanchez-Carrillo[SUP] 7 8 [/SUP], Santiago Moreno[SUP] 4 5 9 [/SUP], Fernando Corrales[SUP] #[/SUP][SUP] 3 [/SUP], Manuel Ferrer[SUP] #[/SUP][SUP] 7 [/SUP], Sergio Serrano-Villar[SUP] #[/SUP][SUP] 4 5 [/SUP]
Affiliations
Background: Information on the microbiome's human pathways and active members that can affect SARS-CoV-2 susceptibility and pathogenesis in the salivary proteome is very scarce. Here, we studied a unique collection of samples harvested from April to June 2020 from unvaccinated patients.
Methods: We compared 10 infected and hospitalized patients with severe (n = 5) and moderate (n = 5) coronavirus disease (COVID-19) with 10 uninfected individuals, including non-COVID-19 but susceptible individuals (n = 5) and non-COVID-19 and nonsusceptible healthcare workers with repeated high-risk exposures (n = 5).
Results: By performing high-throughput proteomic profiling in saliva samples, we detected 226 unique differentially expressed (DE) human proteins between groups (q-value ≤ 0.05) out of 3376 unambiguously identified proteins (false discovery rate ≤ 1%). Major differences were observed between the non-COVID-19 and nonsusceptible groups. Bioinformatics analysis of DE proteins revealed human proteomic signatures related to inflammatory responses, central cellular processes, and antiviral activity associated with the saliva of SARS-CoV-2-infected patients (p-value ≤ 0.0004). Discriminatory biomarker signatures from human saliva include cystatins, protective molecules present in the oral cavity, calprotectins, involved in cell cycle progression, and histones, related to nucleosome functions. The expression levels of two human proteins related to protein transport in the cytoplasm, DYNC1 (p-value, 0.0021) and MAPRE1 (p-value, 0.047), correlated with angiotensin-converting enzyme 2 (ACE2) plasma activity. Finally, the proteomes of microorganisms present in the saliva samples showed 4 main microbial functional features related to ribosome functioning that were overrepresented in the infected group.
Conclusion: Our study explores potential candidates involved in pathways implicated in SARS-CoV-2 susceptibility, although further studies in larger cohorts will be necessary.
Keywords: Functional analysis; Pathogenesis; Proteomics; SARS-CoV-2; Saliva.
. 2024 May 22;21(1):37.
doi: 10.1186/s12014-024-09482-9. Proteomic snapshot of saliva samples predicts new pathways implicated in SARS-CoV-2 pathogenesis
Elena Moreno[SUP] #[/SUP][SUP] 1 2 [/SUP], Sergio Ciordia[SUP] #[/SUP][SUP] 3 [/SUP], Santos Milhano Fátima[SUP] #[/SUP][SUP] 3 [/SUP], Daniel Jiménez[SUP] 4 [/SUP], Javier Martínez-Sanz[SUP] 4 5 [/SUP], Pilar Vizcarra[SUP] 4 5 [/SUP], Raquel Ron[SUP] 4 5 [/SUP], Matilde Sánchez-Conde[SUP] 4 5 [/SUP], Rafael Bargiela[SUP] 6 [/SUP], Sergio Sanchez-Carrillo[SUP] 7 8 [/SUP], Santiago Moreno[SUP] 4 5 9 [/SUP], Fernando Corrales[SUP] #[/SUP][SUP] 3 [/SUP], Manuel Ferrer[SUP] #[/SUP][SUP] 7 [/SUP], Sergio Serrano-Villar[SUP] #[/SUP][SUP] 4 5 [/SUP]
Affiliations
- PMID: 38778280
- DOI: 10.1186/s12014-024-09482-9
Background: Information on the microbiome's human pathways and active members that can affect SARS-CoV-2 susceptibility and pathogenesis in the salivary proteome is very scarce. Here, we studied a unique collection of samples harvested from April to June 2020 from unvaccinated patients.
Methods: We compared 10 infected and hospitalized patients with severe (n = 5) and moderate (n = 5) coronavirus disease (COVID-19) with 10 uninfected individuals, including non-COVID-19 but susceptible individuals (n = 5) and non-COVID-19 and nonsusceptible healthcare workers with repeated high-risk exposures (n = 5).
Results: By performing high-throughput proteomic profiling in saliva samples, we detected 226 unique differentially expressed (DE) human proteins between groups (q-value ≤ 0.05) out of 3376 unambiguously identified proteins (false discovery rate ≤ 1%). Major differences were observed between the non-COVID-19 and nonsusceptible groups. Bioinformatics analysis of DE proteins revealed human proteomic signatures related to inflammatory responses, central cellular processes, and antiviral activity associated with the saliva of SARS-CoV-2-infected patients (p-value ≤ 0.0004). Discriminatory biomarker signatures from human saliva include cystatins, protective molecules present in the oral cavity, calprotectins, involved in cell cycle progression, and histones, related to nucleosome functions. The expression levels of two human proteins related to protein transport in the cytoplasm, DYNC1 (p-value, 0.0021) and MAPRE1 (p-value, 0.047), correlated with angiotensin-converting enzyme 2 (ACE2) plasma activity. Finally, the proteomes of microorganisms present in the saliva samples showed 4 main microbial functional features related to ribosome functioning that were overrepresented in the infected group.
Conclusion: Our study explores potential candidates involved in pathways implicated in SARS-CoV-2 susceptibility, although further studies in larger cohorts will be necessary.
Keywords: Functional analysis; Pathogenesis; Proteomics; SARS-CoV-2; Saliva.