tetano
Editor, Senior Moderator
Clin Microbiol Infect
. 2020 Sep 5;S1198-743X(20)30527-9.
doi: 10.1016/j.cmi.2020.08.043. Online ahead of print.
The kinetics of viral load and antibodiesto SARS-CoV-2
Jiufeng Sun[SUP] 1 [/SUP], Xi Tang[SUP] 2 [/SUP], Ru Bai[SUP] 3 [/SUP], Chumin Liang[SUP] 1 [/SUP], Lilian Zeng[SUP] 1 [/SUP], Huifang Lin[SUP] 1 [/SUP], Runyu Yuan[SUP] 1 [/SUP], Pingping Zhou[SUP] 1 [/SUP], Xuhe Huang[SUP] 1 [/SUP], Qianlin Xiong[SUP] 1 [/SUP], Jinju Peng[SUP] 1 [/SUP], Fengfu Cui[SUP] 1 [/SUP], Bixia Ke[SUP] 4 [/SUP], Juan Su[SUP] 4 [/SUP], Zhe Liu[SUP] 1 [/SUP], Jing Lu[SUP] 1 [/SUP], Junzhang Tian[SUP] 3 [/SUP], Ruilin Sun[SUP] 5 [/SUP], Changwen Ke[SUP] 6 [/SUP]
Affiliations
Abstract
Objectives: To understand persistence of the virus in body fluids and immune response of infected host to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), an agent of coronavirus disease 2019 (COVID-19).
Methods: We determined the kinetics of viral load in several body fluids through real time reverse transcription polymerase chain reaction (rRT-PCR), serum antibodies of IgA, IgG and IgM by enzyme linked immunosorbent assay (ELISA), and neutralizing antibodies by microneutralization assay in 35 COVID-19 cases from two hospitals in Guangdong, China.
Results: We found higher viral loads and prolonged shedding of virus RNA in severe cases of COVID-19 in nasopharyngeal (1.3?10[SUP]6[/SUP] vs 6.4?10[SUP]4[/SUP], p<0.05; 7∼8w) and throat (6.9?10[SUP]6[/SUP] vs 2.9?10[SUP]5[/SUP], p<0.05; 4∼5w), while comparable in sputum samples (5.5?10[SUP]6[/SUP] vs 0.9?10[SUP]6[/SUP], p<0.05; 4∼5w). Viraemia was rarely detected (2.8%, n=1/35). We detected early seroconversion of IgA and IgG at 1[SUP]st[/SUP] week after illness onset (day 5, 5.7%, n=2/35). Neutralizing antibodies were produced in the second week, and observed in all 35 included cases after 3[SUP]rd[/SUP] week illness onset. The levels of neutralizing antibodies correlated with IgG (r[SUB]s[/SUB]=0.85, p<0.05; kappa=0.85) and IgA (r[SUB]s[/SUB]=0.64, p<0.05; kappa=0.61) in severe, but not mild cases (IgG, r[SUB]s[/SUB]=0.42, kappa=0.33; IgA, r[SUB]s[/SUB]=0.32, kappa=0.22). No correlation with IgM in either severe (r[SUB]s[/SUB]=0.17, kappa=0.06) or mild cases (r[SUB]s[/SUB]=0.27, kappa=0.15) was found.
Conclusions: We revealed a prolonged shedding of virus RNA in upper respiratory tract, and evaluated the consistency production of IgG, IgA, IgM and neutralizing antibodies in COVID-19 cases.
Keywords: COVID-19; IgA; IgG; IgM; SARS-CoV-2; neutralizing antibodies; viral load.
. 2020 Sep 5;S1198-743X(20)30527-9.
doi: 10.1016/j.cmi.2020.08.043. Online ahead of print.
The kinetics of viral load and antibodiesto SARS-CoV-2
Jiufeng Sun[SUP] 1 [/SUP], Xi Tang[SUP] 2 [/SUP], Ru Bai[SUP] 3 [/SUP], Chumin Liang[SUP] 1 [/SUP], Lilian Zeng[SUP] 1 [/SUP], Huifang Lin[SUP] 1 [/SUP], Runyu Yuan[SUP] 1 [/SUP], Pingping Zhou[SUP] 1 [/SUP], Xuhe Huang[SUP] 1 [/SUP], Qianlin Xiong[SUP] 1 [/SUP], Jinju Peng[SUP] 1 [/SUP], Fengfu Cui[SUP] 1 [/SUP], Bixia Ke[SUP] 4 [/SUP], Juan Su[SUP] 4 [/SUP], Zhe Liu[SUP] 1 [/SUP], Jing Lu[SUP] 1 [/SUP], Junzhang Tian[SUP] 3 [/SUP], Ruilin Sun[SUP] 5 [/SUP], Changwen Ke[SUP] 6 [/SUP]
Affiliations
- PMID: 32898715
- DOI: 10.1016/j.cmi.2020.08.043
Abstract
Objectives: To understand persistence of the virus in body fluids and immune response of infected host to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), an agent of coronavirus disease 2019 (COVID-19).
Methods: We determined the kinetics of viral load in several body fluids through real time reverse transcription polymerase chain reaction (rRT-PCR), serum antibodies of IgA, IgG and IgM by enzyme linked immunosorbent assay (ELISA), and neutralizing antibodies by microneutralization assay in 35 COVID-19 cases from two hospitals in Guangdong, China.
Results: We found higher viral loads and prolonged shedding of virus RNA in severe cases of COVID-19 in nasopharyngeal (1.3?10[SUP]6[/SUP] vs 6.4?10[SUP]4[/SUP], p<0.05; 7∼8w) and throat (6.9?10[SUP]6[/SUP] vs 2.9?10[SUP]5[/SUP], p<0.05; 4∼5w), while comparable in sputum samples (5.5?10[SUP]6[/SUP] vs 0.9?10[SUP]6[/SUP], p<0.05; 4∼5w). Viraemia was rarely detected (2.8%, n=1/35). We detected early seroconversion of IgA and IgG at 1[SUP]st[/SUP] week after illness onset (day 5, 5.7%, n=2/35). Neutralizing antibodies were produced in the second week, and observed in all 35 included cases after 3[SUP]rd[/SUP] week illness onset. The levels of neutralizing antibodies correlated with IgG (r[SUB]s[/SUB]=0.85, p<0.05; kappa=0.85) and IgA (r[SUB]s[/SUB]=0.64, p<0.05; kappa=0.61) in severe, but not mild cases (IgG, r[SUB]s[/SUB]=0.42, kappa=0.33; IgA, r[SUB]s[/SUB]=0.32, kappa=0.22). No correlation with IgM in either severe (r[SUB]s[/SUB]=0.17, kappa=0.06) or mild cases (r[SUB]s[/SUB]=0.27, kappa=0.15) was found.
Conclusions: We revealed a prolonged shedding of virus RNA in upper respiratory tract, and evaluated the consistency production of IgG, IgA, IgM and neutralizing antibodies in COVID-19 cases.
Keywords: COVID-19; IgA; IgG; IgM; SARS-CoV-2; neutralizing antibodies; viral load.