tetano
Editor, Senior Moderator
Clin Infect Dis
. 2020 Jun 21;ciaa797.
doi: 10.1093/cid/ciaa797. Online ahead of print.
Posterior Oropharyngeal Saliva for the Detection of SARS-CoV-2
Sally Cheuk, Ying Wong[SUP] 1 [/SUP], Herman Tse[SUP] 2 [/SUP], Hon Kei Siu[SUP] 3 [/SUP], Tsz Shan Kwong[SUP] 4 [/SUP], Man Yee Chu[SUP] 4 [/SUP], Felix Yat Sun Yau[SUP] 5 [/SUP], Ingrid Yu Ying Cheung[SUP] 6 [/SUP], Cindy Wing Sze Tse[SUP] 6 [/SUP], Kin Chiu Poon[SUP] 1 [/SUP], Kwok Chi Cheung[SUP] 1 [/SUP], Tak Chiu Wu[SUP] 4 [/SUP], Johnny Wai Man Chan[SUP] 4 [/SUP], Wah Cheuk[SUP] 1 [/SUP], David Christopher Lung[SUP] 1 3 [/SUP]
Affiliations
Abstract
Background: The coronavirus disease-2019 (COVID-19) pandemic has put tremendous pressure on the healthcare system worldwide. Diagnostic testing remained one of the limiting factors for early identification and isolation of infected patients. This study aimed to evaluate posterior oropharyngeal saliva (POPS) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection among patients with confirmed or suspected COVID-19.
Methods: The laboratory information system was searched retrospectively for all respiratory specimens and POPS requested for SARS-CoV-2 RNA detection between Feb 1, 2020 and Apr 15, 2020. The agreement and diagnostic performance of POPS against NPsp were evaluated.
Results: A total of 13772 specimens were identified during the study period, including 2130 POPS and 8438 NPsp. Two hundred and twenty-nine same-day POPS-NPsp paired were identified with POPS and NPsp positivity of 61.5% (95% CI [55.1-67.6%]) and 53.3% (95% CI [46.8-59.6%]). The overall, negative and positive percent agreement were 76.0% (95% CI [70.2-80.9%]), 65.4% (95% CI [55.5-74.2%]), 85.2% (95% CI [77.4-90.8%]). Better positive percent agreement was observed in POPS-NPsp obtained within seven days (96.6%, 95% CI [87.3-99.4%]) compared with after seven days of symptom onset (75.0%, 95% CI [61.4-85.2%)). Among the 104 positive pairs, the mean difference in Cp value was 0.26 (range: 12.63 to -14.74), with an overall higher Cp value in NPsp (Pearson coefficient 0.579). No significant temporal variation was noted between the two specimen types.
Conclusions: POPS is an acceptable alternative specimen to nasopharyngeal specimen for the detection of SARS-CoV-2.
Keywords: COVID-19; mass screening; pandemic; saliva; severe acute respiratory syndrome coronavirus 2.
. 2020 Jun 21;ciaa797.
doi: 10.1093/cid/ciaa797. Online ahead of print.
Posterior Oropharyngeal Saliva for the Detection of SARS-CoV-2
Sally Cheuk, Ying Wong[SUP] 1 [/SUP], Herman Tse[SUP] 2 [/SUP], Hon Kei Siu[SUP] 3 [/SUP], Tsz Shan Kwong[SUP] 4 [/SUP], Man Yee Chu[SUP] 4 [/SUP], Felix Yat Sun Yau[SUP] 5 [/SUP], Ingrid Yu Ying Cheung[SUP] 6 [/SUP], Cindy Wing Sze Tse[SUP] 6 [/SUP], Kin Chiu Poon[SUP] 1 [/SUP], Kwok Chi Cheung[SUP] 1 [/SUP], Tak Chiu Wu[SUP] 4 [/SUP], Johnny Wai Man Chan[SUP] 4 [/SUP], Wah Cheuk[SUP] 1 [/SUP], David Christopher Lung[SUP] 1 3 [/SUP]
Affiliations
- PMID: 32562544
- DOI: 10.1093/cid/ciaa797
Abstract
Background: The coronavirus disease-2019 (COVID-19) pandemic has put tremendous pressure on the healthcare system worldwide. Diagnostic testing remained one of the limiting factors for early identification and isolation of infected patients. This study aimed to evaluate posterior oropharyngeal saliva (POPS) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection among patients with confirmed or suspected COVID-19.
Methods: The laboratory information system was searched retrospectively for all respiratory specimens and POPS requested for SARS-CoV-2 RNA detection between Feb 1, 2020 and Apr 15, 2020. The agreement and diagnostic performance of POPS against NPsp were evaluated.
Results: A total of 13772 specimens were identified during the study period, including 2130 POPS and 8438 NPsp. Two hundred and twenty-nine same-day POPS-NPsp paired were identified with POPS and NPsp positivity of 61.5% (95% CI [55.1-67.6%]) and 53.3% (95% CI [46.8-59.6%]). The overall, negative and positive percent agreement were 76.0% (95% CI [70.2-80.9%]), 65.4% (95% CI [55.5-74.2%]), 85.2% (95% CI [77.4-90.8%]). Better positive percent agreement was observed in POPS-NPsp obtained within seven days (96.6%, 95% CI [87.3-99.4%]) compared with after seven days of symptom onset (75.0%, 95% CI [61.4-85.2%)). Among the 104 positive pairs, the mean difference in Cp value was 0.26 (range: 12.63 to -14.74), with an overall higher Cp value in NPsp (Pearson coefficient 0.579). No significant temporal variation was noted between the two specimen types.
Conclusions: POPS is an acceptable alternative specimen to nasopharyngeal specimen for the detection of SARS-CoV-2.
Keywords: COVID-19; mass screening; pandemic; saliva; severe acute respiratory syndrome coronavirus 2.