tetano
Editor, Senior Moderator
Clin Infect Dis. 2020 Apr 20. pii: ciaa462. doi: 10.1093/cid/ciaa462. [Epub ahead of print]
Down-regulated gene expression spectrum and immune responses changed during the disease progression in COVID-19 patients.
Ouyang Y[SUP]1,[/SUP][SUP]2[/SUP], Yin J[SUP]1,[/SUP][SUP]2[/SUP], Wang W[SUP]1,[/SUP][SUP]2[/SUP], Shi H[SUP]1,[/SUP][SUP]2[/SUP], Shi Y[SUP]1,[/SUP][SUP]2[/SUP], Xu B[SUP]1[/SUP], Qiao L[SUP]1,[/SUP][SUP]2[/SUP], Feng Y[SUP]1[/SUP], Pang L[SUP]1,[/SUP][SUP]2[/SUP], Wei F[SUP]1,[/SUP][SUP]2[/SUP], Guo X[SUP]1,[/SUP][SUP]2[/SUP], Jin R[SUP]1,[/SUP][SUP]2[/SUP], Chen D[SUP]1,[/SUP][SUP]2[/SUP].
Author information
Abstract
BACKGROUND:
WHO characterizes novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a pandemic. Here, we investigated the clinical, cytokine levels, T cell proportion and related gene expression occurring in COVID-19 patients on admission and after intial treatment.
METHODS:
11 patients diagnosed as COVID-19 with similar initial treatment regimen were enrolled in the hospital. Plasma cytokines, CyTOF and microfluidic qPCR for gene expression were conducted.
RESULTS:
5 mild and 6 severe patients were included. Cough and fever were the top symptoms in the 11 COVID-2019 cases. The elder age, more neutrophils numbers and higher C-reactive protein level were found in severe cases. IL-10 level was significantly varied with disease progression and treatment. The decreased T cell proportions were observed in COVID-19 patients especially in severe cases, and all elevated to normal in mild patiens after initial treatment but only CD4+T cells return to normal in severe cases. The number of DEGs increased with the disease progress, and decreased after initial treatment. All down-regulated DEGs in severe cases mainly involved in Th17 cell differentiation, cytokine-mediated signaling pathway and T cell activation. After initial treatmen in severe cases, MAP2K7 and SOS1 were upregulated relative to that on admission.
CONCLUSIONS:
Our findings show a decreased T cell proportion with down-regulated gene expression related to T cell activation and differentiation were occurred in COVID-19 severe patients, which may help to provide effective treatment strategies for COVID-19 .
? The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.
KEYWORDS:
COVID-2019; Cytokine; Gene expression; Immune response; PBMC
PMID:32307550DOI:10.1093/cid/ciaa462
Down-regulated gene expression spectrum and immune responses changed during the disease progression in COVID-19 patients.
Ouyang Y[SUP]1,[/SUP][SUP]2[/SUP], Yin J[SUP]1,[/SUP][SUP]2[/SUP], Wang W[SUP]1,[/SUP][SUP]2[/SUP], Shi H[SUP]1,[/SUP][SUP]2[/SUP], Shi Y[SUP]1,[/SUP][SUP]2[/SUP], Xu B[SUP]1[/SUP], Qiao L[SUP]1,[/SUP][SUP]2[/SUP], Feng Y[SUP]1[/SUP], Pang L[SUP]1,[/SUP][SUP]2[/SUP], Wei F[SUP]1,[/SUP][SUP]2[/SUP], Guo X[SUP]1,[/SUP][SUP]2[/SUP], Jin R[SUP]1,[/SUP][SUP]2[/SUP], Chen D[SUP]1,[/SUP][SUP]2[/SUP].
Author information
Abstract
BACKGROUND:
WHO characterizes novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a pandemic. Here, we investigated the clinical, cytokine levels, T cell proportion and related gene expression occurring in COVID-19 patients on admission and after intial treatment.
METHODS:
11 patients diagnosed as COVID-19 with similar initial treatment regimen were enrolled in the hospital. Plasma cytokines, CyTOF and microfluidic qPCR for gene expression were conducted.
RESULTS:
5 mild and 6 severe patients were included. Cough and fever were the top symptoms in the 11 COVID-2019 cases. The elder age, more neutrophils numbers and higher C-reactive protein level were found in severe cases. IL-10 level was significantly varied with disease progression and treatment. The decreased T cell proportions were observed in COVID-19 patients especially in severe cases, and all elevated to normal in mild patiens after initial treatment but only CD4+T cells return to normal in severe cases. The number of DEGs increased with the disease progress, and decreased after initial treatment. All down-regulated DEGs in severe cases mainly involved in Th17 cell differentiation, cytokine-mediated signaling pathway and T cell activation. After initial treatmen in severe cases, MAP2K7 and SOS1 were upregulated relative to that on admission.
CONCLUSIONS:
Our findings show a decreased T cell proportion with down-regulated gene expression related to T cell activation and differentiation were occurred in COVID-19 severe patients, which may help to provide effective treatment strategies for COVID-19 .
? The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.
KEYWORDS:
COVID-2019; Cytokine; Gene expression; Immune response; PBMC
PMID:32307550DOI:10.1093/cid/ciaa462