tetano
Editor, Senior Moderator
Clin Exp Nephrol
. 2022 Nov 7.
doi: 10.1007/s10157-022-02296-9. Online ahead of print.
Establishment and clinical application of SARS-CoV-2 catch column
Yoshitaka Isaka[SUP] #[/SUP][SUP] 1 [/SUP], Taku Yoshiya[SUP] #[/SUP][SUP] 2 3 [/SUP], Chikako Ono[SUP] #[/SUP][SUP] 4 5 [/SUP], Akinori Uchiyama[SUP] 6 [/SUP], Haruhiko Hirata[SUP] 7 [/SUP], Shigeto Hamaguchi[SUP] 8 [/SUP], Satoshi Kutsuna[SUP] 8 [/SUP], Yoshitsugu Takabatake[SUP] 9 [/SUP], Ryotaro Saita[SUP] 10 [/SUP], Tomomi Yamada[SUP] 10 [/SUP], Atsushi Takahashi[SUP] 9 [/SUP], Masaya Yamato[SUP] 11 [/SUP], Yukie Nohara[SUP] 2 [/SUP], Shugo Tsuda[SUP] 2 [/SUP], Itsuki Anzai[SUP] 12 [/SUP], Tomonori Kimura[SUP] 13 [/SUP], Yoshito Takeda[SUP] 7 [/SUP], Kazunori Tomono[SUP] 14 [/SUP], Yoshiharu Matsuura[SUP] 4 5 [/SUP]
Affiliations
Abstract
Background: A certain number of patients with coronavirus disease 2019 (COVID-19), particularly those who test positive for SARS-CoV-2 in the serum, are hospitalized. Further, some even die. We examined the effect of blood adsorption therapy using columns that can eliminate SARS-CoV-2 on the improvement of the prognosis of severe COVID-19 patients.
Methods: This study enrolled seven patients receiving mechanical ventilation. The patients received viral adsorption therapy using SARS-catch column for 3 days. The SARS-catch column was developed by immobilizing a specific peptide, designed based on the sequence of human angiotensin-converting enzyme 2 (hACE2), to an endotoxin adsorption column (PMX). In total, eight types of SARS-CoV-2-catch (SCC) candidate peptides were developed. Then, a clinical study on the effects of blood adsorption therapy using the SARS-catch column in patients with severe COVID-19 was performed, and the data in the present study were compared with historical data of severe COVID-19 patients.
Results: Among all SCC candidate peptides, SCC-4N had the best adsorption activity against SARS-CoV-2. The SARS-catch column using SCC-4N removed 65% more SARS-CoV-2 than PMX. Compared with historical data, the weaning time from mechanical ventilation was faster in the present study. In addition, the rate of negative blood viral load in the present study was higher than that in the historical data.
Conclusion: The timely treatment with virus adsorption therapy may eliminate serum SARS-CoV-2 and improve the prognosis of patients with severe COVID-19. However, large-scale studies must be performed in the future to further assess the finding of this study (jRCTs052200134).
Keywords: COVID-19; Clinical study; Viral adsorption therapy.
. 2022 Nov 7.
doi: 10.1007/s10157-022-02296-9. Online ahead of print.
Establishment and clinical application of SARS-CoV-2 catch column
Yoshitaka Isaka[SUP] #[/SUP][SUP] 1 [/SUP], Taku Yoshiya[SUP] #[/SUP][SUP] 2 3 [/SUP], Chikako Ono[SUP] #[/SUP][SUP] 4 5 [/SUP], Akinori Uchiyama[SUP] 6 [/SUP], Haruhiko Hirata[SUP] 7 [/SUP], Shigeto Hamaguchi[SUP] 8 [/SUP], Satoshi Kutsuna[SUP] 8 [/SUP], Yoshitsugu Takabatake[SUP] 9 [/SUP], Ryotaro Saita[SUP] 10 [/SUP], Tomomi Yamada[SUP] 10 [/SUP], Atsushi Takahashi[SUP] 9 [/SUP], Masaya Yamato[SUP] 11 [/SUP], Yukie Nohara[SUP] 2 [/SUP], Shugo Tsuda[SUP] 2 [/SUP], Itsuki Anzai[SUP] 12 [/SUP], Tomonori Kimura[SUP] 13 [/SUP], Yoshito Takeda[SUP] 7 [/SUP], Kazunori Tomono[SUP] 14 [/SUP], Yoshiharu Matsuura[SUP] 4 5 [/SUP]
Affiliations
- PMID: 36344716
- DOI: 10.1007/s10157-022-02296-9
Abstract
Background: A certain number of patients with coronavirus disease 2019 (COVID-19), particularly those who test positive for SARS-CoV-2 in the serum, are hospitalized. Further, some even die. We examined the effect of blood adsorption therapy using columns that can eliminate SARS-CoV-2 on the improvement of the prognosis of severe COVID-19 patients.
Methods: This study enrolled seven patients receiving mechanical ventilation. The patients received viral adsorption therapy using SARS-catch column for 3 days. The SARS-catch column was developed by immobilizing a specific peptide, designed based on the sequence of human angiotensin-converting enzyme 2 (hACE2), to an endotoxin adsorption column (PMX). In total, eight types of SARS-CoV-2-catch (SCC) candidate peptides were developed. Then, a clinical study on the effects of blood adsorption therapy using the SARS-catch column in patients with severe COVID-19 was performed, and the data in the present study were compared with historical data of severe COVID-19 patients.
Results: Among all SCC candidate peptides, SCC-4N had the best adsorption activity against SARS-CoV-2. The SARS-catch column using SCC-4N removed 65% more SARS-CoV-2 than PMX. Compared with historical data, the weaning time from mechanical ventilation was faster in the present study. In addition, the rate of negative blood viral load in the present study was higher than that in the historical data.
Conclusion: The timely treatment with virus adsorption therapy may eliminate serum SARS-CoV-2 and improve the prognosis of patients with severe COVID-19. However, large-scale studies must be performed in the future to further assess the finding of this study (jRCTs052200134).
Keywords: COVID-19; Clinical study; Viral adsorption therapy.