tetano
Editor, Senior Moderator
Clin Chem Lab Med
. 2021 Apr 12.
doi: 10.1515/cclm-2021-0339. Online ahead of print.
Comprehensive assessment of humoral response after Pfizer BNT162b2 mRNA Covid-19 vaccination: a three-case series
Elisa Danese[SUP] 1 [/SUP], Martina Montagnana[SUP] 1 [/SUP], Gian Luca Salvagno[SUP] 1 2 [/SUP], Denise Peserico[SUP] 1 [/SUP], Laura Pighi[SUP] 1 [/SUP], Simone De Nitto[SUP] 1 [/SUP], Brandon M Henry[SUP] 3 [/SUP], Stefano Porru[SUP] 4 [/SUP], Giuseppe Lippi[SUP] 1 [/SUP]
Affiliations
Abstract
Objectives: Since universal vaccination is a pillar against coronavirus disease 2019 (COVID-19), monitoring anti-SARS-CoV-2 neutralizing antibodies is essential for deciphering post-vaccination immune response.
Methods: Three healthcare workers received 30 ?g BNT162b2 mRNA Covid-19 Pfizer Vaccine, followed by a second identical dose, 21 days afterwards. Venous blood was drawn at baseline and at serial intervals, up to 63 days afterwards, for assessing total immunoglobulins (Ig) anti-RBD (receptor binding domain), anti-S1/S2 and anti-RBD IgG, anti-RBD and anti-N/S1 IgM, and anti-S1 IgA.
Results: All subjects were SARS-CoV-2 seronegative at baseline. Total Ig anti-RBD, anti-S1/S2 and anti-RBD IgG levels increased between 91 and 368 folds until 21 days after the first vaccine dose, then reached a plateau. The levels raised further after the second dose (by ?30-, ?8- and ?8-fold, respectively), peaking at day 35, but then slightly declining and stabilizing ?50 days after the first vaccine dose. Anti-S1 IgA levels increased between 7 and 11 days after the first dose, slightly declined before the second dose, after which levels augmented by ?24-fold from baseline. The anti-RBD and anti-N/S1 IgM kinetics were similar to that of anti-S1 IgA, though displaying substantially weaker increases and modest peaks, only 4- to 7-fold higher than baseline. Highly significant inter-correlation was noted between total Ig anti-RBD, anti-S1/S2 and anti-RBD IgG (all r=0.99), whilst other anti-SARS-CoV-2 antibodies displayed lower, though still significant, correlations. Serum spike protein concentration was undetectable at all-time points.
Conclusions: BNT162b2 mRNA vaccination generates a robust humoral immune response, especially involving anti-SARS-Cov-2 IgG and IgA, magnified by the second vaccine dose.
Keywords: COVID-19; antibodies; coronavirus; immune response; vaccine.
. 2021 Apr 12.
doi: 10.1515/cclm-2021-0339. Online ahead of print.
Comprehensive assessment of humoral response after Pfizer BNT162b2 mRNA Covid-19 vaccination: a three-case series
Elisa Danese[SUP] 1 [/SUP], Martina Montagnana[SUP] 1 [/SUP], Gian Luca Salvagno[SUP] 1 2 [/SUP], Denise Peserico[SUP] 1 [/SUP], Laura Pighi[SUP] 1 [/SUP], Simone De Nitto[SUP] 1 [/SUP], Brandon M Henry[SUP] 3 [/SUP], Stefano Porru[SUP] 4 [/SUP], Giuseppe Lippi[SUP] 1 [/SUP]
Affiliations
- PMID: 33838087
- DOI: 10.1515/cclm-2021-0339
Abstract
Objectives: Since universal vaccination is a pillar against coronavirus disease 2019 (COVID-19), monitoring anti-SARS-CoV-2 neutralizing antibodies is essential for deciphering post-vaccination immune response.
Methods: Three healthcare workers received 30 ?g BNT162b2 mRNA Covid-19 Pfizer Vaccine, followed by a second identical dose, 21 days afterwards. Venous blood was drawn at baseline and at serial intervals, up to 63 days afterwards, for assessing total immunoglobulins (Ig) anti-RBD (receptor binding domain), anti-S1/S2 and anti-RBD IgG, anti-RBD and anti-N/S1 IgM, and anti-S1 IgA.
Results: All subjects were SARS-CoV-2 seronegative at baseline. Total Ig anti-RBD, anti-S1/S2 and anti-RBD IgG levels increased between 91 and 368 folds until 21 days after the first vaccine dose, then reached a plateau. The levels raised further after the second dose (by ?30-, ?8- and ?8-fold, respectively), peaking at day 35, but then slightly declining and stabilizing ?50 days after the first vaccine dose. Anti-S1 IgA levels increased between 7 and 11 days after the first dose, slightly declined before the second dose, after which levels augmented by ?24-fold from baseline. The anti-RBD and anti-N/S1 IgM kinetics were similar to that of anti-S1 IgA, though displaying substantially weaker increases and modest peaks, only 4- to 7-fold higher than baseline. Highly significant inter-correlation was noted between total Ig anti-RBD, anti-S1/S2 and anti-RBD IgG (all r=0.99), whilst other anti-SARS-CoV-2 antibodies displayed lower, though still significant, correlations. Serum spike protein concentration was undetectable at all-time points.
Conclusions: BNT162b2 mRNA vaccination generates a robust humoral immune response, especially involving anti-SARS-Cov-2 IgG and IgA, magnified by the second vaccine dose.
Keywords: COVID-19; antibodies; coronavirus; immune response; vaccine.