tetano
Editor, Senior Moderator
Hum Immunol. 2012 Aug 16. [Epub ahead of print]
Circulating CD35(-)/CD49d(+) neutrophils in influenza virus infection patients.
Pliyev BK, Shmidt EI, Ivanova AV, Menshikov M.
Source
Laboratory of Clinical Immunology, Institute of Immunology, Kashirskoye Shosse 24-2, Moscow 115478, Russia; Department of Biochemistry, Institute of Experimental Cardiology, Cardiology Research Centre, 3rd Cherepkovskaya Str., 15a, Moscow 121552, Russia.
Abstract
Release of immature neutrophils from bone marrow into circulation accompanies acute inflammatory states. Previous studies have identified a number of antigens that are differentially expressed on mature neutrophils and immature forms. We analyzed expression of these antigens on circulating neutrophils of influenza virus infection patients. We identified a minor neutrophil subpopulation with decreased granularity and the antigen surface expression pattern (CD35(-)/CD49d(+)) which defines metamyelocytes. CD35(-)/CD49d(+) neutrophils were detected in 35% of the patients but not in healthy individuals and correlated positively with blood metamyelocyte count. The sorted CD35(-)/CD49d(+) neutrophils displayed morphological features of metamyelocytes. Compared with a major (CD35(+)/CD49d(-)) neutrophil subpopulation, CD35(-)/CD49d(+) neutrophils demonstrated significantly diminished functional capacities (both phagocytosis and respiratory burst). Thus, CD35(-)/CD49d(+) neutrophils represent a phenotypically and functionally distinct subpopulation of the cells and can potentially be used to quantify immature neutrophil release in inflammation.
Copyright ? 2012. Published by Elsevier Inc.
PMID:
22902398
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22902398
Circulating CD35(-)/CD49d(+) neutrophils in influenza virus infection patients.
Pliyev BK, Shmidt EI, Ivanova AV, Menshikov M.
Source
Laboratory of Clinical Immunology, Institute of Immunology, Kashirskoye Shosse 24-2, Moscow 115478, Russia; Department of Biochemistry, Institute of Experimental Cardiology, Cardiology Research Centre, 3rd Cherepkovskaya Str., 15a, Moscow 121552, Russia.
Abstract
Release of immature neutrophils from bone marrow into circulation accompanies acute inflammatory states. Previous studies have identified a number of antigens that are differentially expressed on mature neutrophils and immature forms. We analyzed expression of these antigens on circulating neutrophils of influenza virus infection patients. We identified a minor neutrophil subpopulation with decreased granularity and the antigen surface expression pattern (CD35(-)/CD49d(+)) which defines metamyelocytes. CD35(-)/CD49d(+) neutrophils were detected in 35% of the patients but not in healthy individuals and correlated positively with blood metamyelocyte count. The sorted CD35(-)/CD49d(+) neutrophils displayed morphological features of metamyelocytes. Compared with a major (CD35(+)/CD49d(-)) neutrophil subpopulation, CD35(-)/CD49d(+) neutrophils demonstrated significantly diminished functional capacities (both phagocytosis and respiratory burst). Thus, CD35(-)/CD49d(+) neutrophils represent a phenotypically and functionally distinct subpopulation of the cells and can potentially be used to quantify immature neutrophil release in inflammation.
Copyright ? 2012. Published by Elsevier Inc.
PMID:
22902398
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22902398