tetano
Editor, Senior Moderator
J Med Virol. 2015 Feb 25. doi: 10.1002/jmv.24135. [Epub ahead of print]
[h=1]Chloroquine enhances replication of influenza A virus A/WSN/33 (H1N1) in dose-, time-, and MOI-dependent manners in human lung epithelial cells A549.[/h] Wu L[SUP]1[/SUP], Dai J, Zhao X, Chen Y, Wang G, Li K.
[h=3]Author information[/h]
[h=3]Abstract[/h] Anti-malaria drug, chloroquine, has been reported to be effective against influenza A virus (IAV) in vitro and used in in-vivo experiments and clinical trial for prevention or treatment of influenza. In this study, it has been shown by immunofluorescence, hemagglutination, and plaque assays that chloroquine enhanced A/WSN/33 (H1N1) replication with pronounced cytopathic effect in dose-, time-, and MOI-dependent manners in human lung epithelial cells A549. Time-of-addition assay showed that inhibitory effect on virus replication by chloroquine pre-treatment was indistinctive, and virus productions were enhanced when the drug was applied after viral adsorption. The effectiveness of chloroquine as an anti-influenza drug is questioned, and caution in its use is recommended. J. Med. Virol. ? 2015 Wiley Periodicals, Inc.
? 2015 Wiley Periodicals, Inc.
[h=4]KEYWORDS:[/h] A549; WSN; chloroquine; influenza A virus; replication
PMID: 25715935 [PubMed - as supplied by publisher]
[h=1]Chloroquine enhances replication of influenza A virus A/WSN/33 (H1N1) in dose-, time-, and MOI-dependent manners in human lung epithelial cells A549.[/h] Wu L[SUP]1[/SUP], Dai J, Zhao X, Chen Y, Wang G, Li K.
[h=3]Author information[/h]
[h=3]Abstract[/h] Anti-malaria drug, chloroquine, has been reported to be effective against influenza A virus (IAV) in vitro and used in in-vivo experiments and clinical trial for prevention or treatment of influenza. In this study, it has been shown by immunofluorescence, hemagglutination, and plaque assays that chloroquine enhanced A/WSN/33 (H1N1) replication with pronounced cytopathic effect in dose-, time-, and MOI-dependent manners in human lung epithelial cells A549. Time-of-addition assay showed that inhibitory effect on virus replication by chloroquine pre-treatment was indistinctive, and virus productions were enhanced when the drug was applied after viral adsorption. The effectiveness of chloroquine as an anti-influenza drug is questioned, and caution in its use is recommended. J. Med. Virol. ? 2015 Wiley Periodicals, Inc.
? 2015 Wiley Periodicals, Inc.
[h=4]KEYWORDS:[/h] A549; WSN; chloroquine; influenza A virus; replication
PMID: 25715935 [PubMed - as supplied by publisher]