China: National Science and Technology Resource Service System for New Coronaviruses - Open Access - Sequences and Primers

[Gert van der Hoek]

introduction

Coronaviruses are a large group of viruses that are known to cause disease. Patients present with different clinical symptoms ranging from the common cold to severe lung infections, such as the Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The new coronavirus 2019-nCov (hereinafter referred to as virus species) discovered in Wuhan is a new coronavirus that has not been found in humans before.

　　The National Science and Technology Resource Service System for New Coronaviruses (hereinafter referred to as the system) is jointly constructed by the National Microbial Scientific Data Center and the National Pathogen Microbial Resource Database, and is organized by the Chinese Center for Disease Control and Prevention, the Chinese Center for Disease Control and Prevention of Viral Disease Control and Prevention, and the Chinese Academy of Sciences The Institute, the Chinese Academy of Sciences Key Laboratory of Pathogen Microbiology and Immunology, the Chinese Center for Disease Control and Prevention-Institute of Microbiology, Chinese Academy of Sciences Joint Research Center for Pathogen Microbial Resources and Big Data, and other units jointly constructed. The system has functions such as virus species information, primer information, and global coronavirus sequence information query and analysis.

　　The system obtained the National Science and Technology Resource Sharing Service Platform, the prevention and treatment of major infectious diseases such as AIDS and viral hepatitis (2018ZX10734404, 2018ZX1010100404), the Chinese Academy of Sciences Earthquake Big Data Pioneer A Project (XDA19050301), and the Chinese Academy of Sciences Strategic Biological Resources Program (KFJ-BRP) And other project support.

　　The system will release authoritative information on scientific and technological resources and scientific data of new coronaviruses in time with the progress of scientific research on new coronaviruses, provide important support for scientific research of new coronaviruses, and prevent and control the pneumonia epidemic in response to current new coronavirus infections. Provide special services for scientific and technological resources.

 

[Gert van der Hoek]

Virus Information 01

02
Virus number:

　　　　　CHPC 2020.00001; NPRC 2020.00001



Chinese name:

　　　　　New coronavirus Wuhan strain 01



English name:

　　　　　C-Tan-nCov Wuhan strain 01



Taxonomy:

　　　　　2019-nCoV, Coronavirus Beta



Source of separation:

　　　　　Clinical patient



Collection place:

　　　　　Wuhan, Hubei, China



Separation time:

　　　　　January 6, 2020



Degree of biological hazard:

　　　　　Second class



Contact Email:

　　　　　ivdcolm@ivdc.chinacdc.cn; chpc@chinacdc.cn



Photo source:

　　　　　National Pathogen Library

　　　　　(China Center for Disease Control and Prevention, Chinese Center for Disease Control and Prevention)

 

[Gert van der Hoek]

Virus Information 02
Virus number:

　　　　　CHPC 2020.00002; NPRC 2020.00002



Chinese name:

　　　　　New coronavirus Wuhan strain 02



English name:

　　　　　C-F13-nCoV Wuhan strain 02



Taxonomy:

　　　　　2019-nCoV, Coronavirus ?



Source of separation:

　　　　　Environmental sample



Collection place:

　　　　　Wuhan, Hubei, China



Separation time:

　　　　　January 22, 2020



Degree of biological hazard:

　　　　　Second class



Contact Email:

　　　　　ivdcolm@ivdc.chinacdc.cn; chpc@chinacdc.cn



Photo source:

　　　　　National Pathogen Library

　　　　　(China Center for Disease Control and Prevention, Chinese Center for Disease Control and Prevention)

 

[Gert van der Hoek]

Sharing instructions
03


　　The new coronavirus species can be shared for external use (signing a sharing agreement) if they meet the relevant regulations. In the process of using the virus, the user must mark the source of the virus on the relevant materials (including but not limited to papers, patents, etc.): the National Pathogen Microbial Resource Bank (China Center for Disease Control and Prevention of Viral Disease Control and Prevention) and NPRC Numbering.



　　After using the virus-related activities, the user should return the virus to the national pathogenic microorganism resource bank (China Center for Disease Control and Prevention of Viral Disease Control and Prevention) or destroy it on-site, and issue a certificate of destruction and use to the national pathogenic microorganism resource bank Instructions.



　　Relevant achievements and scientific data (including but not limited to papers, patents, etc.) formed by the user shall be submitted to the National Pathogen Microbial Resource Database and the National Microbial Scientific Data Center.

 

[Gert van der Hoek]

New Coronavirus Nucleic Acid Detection Primer and Probe Sequence

Information Source: Chinese Center for Disease Control and Prevention
04
Novel primers and probes for detection of novel coronavirus in coronavirus



1. New Coronavirus Nucleic Acid Assay (Real-time fluorescent RT-PCR method)



Primers and probes targeting the open reading frame (ORF1ab) and nucleoprotein (N) gene regions of the new coronavirus are recommended.



Target 1 (ORF1ab):



Forward primer (F): CCCTGTGGGTTTTACACTTAA



Reverse primer (R): ACGATTGTGCATCAGCTGA



Fluorescent Probe (P): 5'-FAM-CCGTCTGCGGTATGTGGAAAGGTTATGG-BHQ1-3 '



Target 2 (N):



Forward primer (F): GGGGAACTTCTCCTGCTAGAAT



Reverse primer (R): CAGACATTTTGCTCTCAAGCTG



Fluorescent Probe (P): 5'-FAM-TTGCTGCTGCTTGACAGATT-TAMRA-3 '



For nucleic acid extraction and real-time fluorescent RT-PCR reaction system, refer to the relevant manufacturer's kit instructions.



2. Judgment of results



Negative: No Ct value or Ct is 40.



Positive: Ct value <37, can be reported as positive.



Suspicious: The Ct value is between 37-40. It is recommended to repeat the experiment. If the Ct value is less than 40, the amplification curve has obvious peaks.

 

[Gert van der Hoek]

Note: please follow link and scroll down for the sequence information .

Global Coronavirus Sequence Information

06

All fields [TABLE="border: 0, cellpadding: 0, cellspacing: 0"]
[TR]
[/TR]
[/TABLE]
[TABLE="border: 0, cellpadding: 0, cellspacing: 0"]
[TR]
[TD]MN908947[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]Wuhan-Hu-1[/TD]
[TD]29875[/TD]
[TD]2020[/TD]
[TD]View[/TD]
[/TR]
[TR]
[TD]NC_045512[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]Wuhan-Hu-1[/TD]
[TD]30473[/TD]
[TD]2020[/TD]
[TD]View[/TD]
[/TR]
[TR]
[TD]MN975262[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]2019-nCoV_HKU-SZ-005b_2020[/TD]
[TD]29891[/TD]
[TD]2020[/TD]
[TD]View[/TD]
[/TR]
[TR]
[TD]MN938384[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]2019-nCoV_HKU-SZ-002a_2020[/TD]
[TD]29838[/TD]
[TD]2020[/TD]
[TD]View[/TD]
[/TR]
[TR]
[TD]GWHABKG00000000[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]IPBCAMS-WH-02[/TD]
[TD]29889[/TD]
[TD]2020[/TD]
[TD]View[/TD]
[/TR]
[TR]
[TD]GWHABKH00000000[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]IPBCAMS-WH-03[/TD]
[TD]29899[/TD]
[TD]2020[/TD]
[TD]View[/TD]
[/TR]
[TR]
[TD]GWHABKI00000000[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]IPBCAMS-WH-04[/TD]
[TD]29890[/TD]
[TD]2020[/TD]
[TD]View[/TD]
[/TR]
[TR]
[TD]GWHABKF00000000[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]IPBCAMS-WH-01[/TD]
[TD]29899[/TD]
[TD]2020[/TD]
[TD]View[/TD]
[/TR]
[TR]
[TD]GWHABKJ00000000[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]IPBCAMS-WH-05[/TD]
[TD]29883[/TD]
[TD]2020[/TD]
[/TR]
[/TABLE]

 

[Gert van der Hoek]

As an example this sequence is published on the page: http://nmdc.cn/

(Original layout on the website is much better!)
[TABLE="border: 0, cellpadding: 0, cellspacing: 0"]
[TR]
[TD]MN908947[/TD]
[TD]Wuhan seafood market pneumonia virus[/TD]
[TD]Wuhan-Hu-1[/TD]
[TD]29875[/TD]
[TD]2020[/TD]
[/TR]
[/TABLE]
LOCUS MN908947 29875 bp ss-RNA linear VRL 14-JAN-2020
DEFINITION Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1, complete genome.
ACCESSION MN908947 VERSION MN908947.2 KEYWORDS .
SOURCE Wuhan seafood market pneumonia virus ORGANISM Wuhan seafood market pneumonia virus Viruses; Riboviria; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; unclassified Betacoronavirus.
REFERENCE 1 (bases 1 to 29875) AUTHORS Zhang,Y.-Z., Wu,F., Chen,Y.-M., Pei,Y.-Y., Xu,L., Wang,W., Zhao,S., Yu,B., Hu,Y., Tao,Z.-W., Song,Z.-G., Tian,J.-H., Zhang,Y.-L., Liu,Y., Zheng,J.-J., Dai,F.-H., Wang,Q.-M., She,J.-L. and Zhu,T.-Y.
TITLE A novel coronavirus associated with a respiratory disease in Wuhan of Hubei province, China
JOURNAL Unpublished

REFERENCE 2 (bases 1 to 29875) AUTHORS Zhang,Y.-Z., Wu,F., Chen,Y.-M., Pei,Y.-Y., Xu,L., Wang,W., Zhao,S., Yu,B., Hu,Y., Tao,Z.-W., Song,Z.-G., Tian,J.-H., Zhang,Y.-L., Liu,Y., Zheng,J.-J., Dai,F.-H., Wang,Q.-M., She,J.-L. and Zhu,T.-Y. TITLE Direct Submission JOURNAL Submitted (05-JAN-2020) Department of Zoonoses, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, Beijing 102206, China COMMENT [WARNING] On Jan 17, 2020 this sequence was replaced by MN908947.3. On Jan 14, 2020 this sequence version replaced MN908947.1. ##Assembly-Data-START## Assembly Method :: Megahit v. V1.1.3 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..29875 /organism="Wuhan seafood market pneumonia virus" /mol_type="genomic RNA" /isolate="Wuhan-Hu-1" /host="Homo sapiens" /db_xref="taxon:2697049" /country="China" /collection_date="Dec-2019" 5'UTR 1..281 gene 282..21571 /gene="orf1ab" CDS join(282..13484,13484..21571) /gene="orf1ab" /ribosomal_slippage /note="pp1ab; translated by -1 ribosomal frameshift" /codon_start=1 /product="ORF1ab polyprotein" /protein_id="QHD43415.1" /translation="MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEE VLSEARQ HLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEG IQYGRSGE TLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTD PYEDFQEN WNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGK ASCTLSEQ LDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNG ECPNFVFP LNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHC GETSWQTG DFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSL AEYHNESG LKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEG SEGLNDNL LEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFK QIVESCGN FKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNS VRVLQKAA ITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNI FGTVYEKL KPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKES VQTFFKLV NKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMP LKAPKEII FLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLML LEIKDTEK YCALAPNMMVTNNTFTLKGGAPTKVTFGDDTVIEVQGYKSVNITFELDER IDKVLNEK CSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYY LFDESGEF KLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGA TSAALQPE EEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQT IEVNSFSG YLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATN NAMQVESD DYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENF NQHEVLLA PLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSE KQVEQKIA EIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLL LYIDINGN LHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEM LAKALRKV PTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGT VSWNLREM LAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSK TTVASLIN TLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAY NGYLTSSS KTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTF HLDGEVIT FDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGA DVTKIKPH NSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQ VNGLTSIK WADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYC NKTVGELG DVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSY EQFKKGVQ IPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCG HYKHITSK ETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEI DPKLDNYY KKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKP ASRELKVT FFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTW CIRCLWST KPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNV KTTEVVGD IILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLAT HGLAAVNS VPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTF TRSTNSRI KASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCL GSLIYSTA ALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSL DTYPSLET IQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAV HFISNSWL MWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYK RNRATRVE CTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARD LSLQFKRP INPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRAN NTKGSLPI NVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAV KMFDAYVN TFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDV ETKDVVEC LKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVA KSHNIALI WNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKG GKIVNNWL KQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDI ASTDTCFA NKHADFDTWFSQRGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTN GDFLHFLP RVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDT NVLEGSVA YESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEA GVCVSTSG RWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGI VAIVVTCL AYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYL YLTFYLTN DVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFN GVSFSTFE EAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSY REAACCHL AKALNDFSNSGSDVLYQPPQTSITSAVLQSGFRKMAFPSGKVEGCMVQVT CGTTTLNG LWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIG HSMQNCVL KLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIK GSFLNGSC GSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAG TDTTITVN VLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPL SAQTGIAV LDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQSAVKR TIKGTHHW LLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHK HAFLCLFL LPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVV LLILMTAR TVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVV TTVMFLAR GIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRL TLGVYDYL VSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQSKMSD VKCTSVVL LSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQ GAVDINKL CEEMLDNRATLQAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKL KKSLNVAK SEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLR KLDNDALN NIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALW EIQQVVDA DSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQNNELSPVALRQMSC AAGTTQTA CTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPP CRFVTDTP KGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQAGNATEVPANSTVLSFC AFAVDAAK AYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCL YCRCHIDH PNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQL REPMLQSA DAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTN CCRFQEKD EDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDGDM VPHISRQR LTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVEN PDILRVYA NLGERVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTT PGSGVPVV DSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFD RYFKYWDQ TYHPNCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVV STGYHFRE LGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAA LTNNVAFQ TVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRY NLPTMCDI RQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYY DSMSYEDQ DALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQK LLKSIAAT RGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRI MASLVLAR KHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYA NSVFNICQ AVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAY LRKHFSMM ILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDLT KGPHEFCS QHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAI DAYPLTKH PNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYE AMYTPHTV LQAVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPY VCNAPGCD VTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDF NAIATCDW TNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELH LSWEVGKP RPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVG DYFVLTSH TVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTL QGPPGTGK SHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARA RVECFDKF KVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHY VYIGDPAQ LPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSA LVYDNKLK AHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFI SPYNSQNA VASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKV GILCIMSD RDLYDKLQFTSLEIPRRNVATLQAENVTGLFKDCSKVITGLHPTQAPTHL SVDTKFKT EGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRA WIGFDVEG CHATREAVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPG DQFKHLIP LMYKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFELTSMKYFVKI GPERTCCL CDRRATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGNLQSNHDL YCQVHGNA HVASCDAIMTRCLAVHECFVKRVDWTIEYPIIGDELKINAACRKVQHMVV KAALLADK FPVLHDIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSD KFTDGVCL FWNCNVDRYPANSIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDK SAFVNLKQ LPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEY RLYLDAYN MMISAGFSLWVYKQFDTYNLWNTFTRLQSLENVAFNVVNKGHFDGQQGEV PVSIINNT VYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIA ANTVIWDY KRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNAR NGVLITEG SVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQ SRNLQEFK PRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGL AKRFKESP FELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLS VVSKVVKV TIDYTEISFMLWCKDGHVETFYPKLQSSQAWQPGVAMPNLYKMQRMLLEK CDLQNYGD SATLPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGT AVLRQWLP TGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVT KENDSKEG FFTYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVN ASSSEAFL IGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRG TAVMSLKE GQINDMILSLLSKGRLIIRENNRVVISSDVLVNN" gene 21579..25400 /gene="S" CDS 21579..25400 /gene="S" /note="structural protein" /codon_start=1 /product="surface glycoprotein" /protein_id="QHD43416.1" /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVY YPDKVFR SSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFAS TEKSNIIR GWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSW MESEFRVY SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPI NLVRDLPQ GFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVG YLQPRTFL LKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESI VRFPNITN LCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSP TKLNDLCF TNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLD SKVGGNYN YLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPT NGVGYQPY RVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKK FLPFQQFG RDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVN CTEVPVAI HADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASY QTQTNSPR RARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMT KTSVDCTM YICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYK TPPIKDFG GFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARD LICAQKFN GLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRF NGIGVTQN VLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVK QLSSNFGA ISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASA NLAATKMS ECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPA ICHDGKAH FPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVY DPLQPELD SFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNES LIDLQELG KYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCC KFDEDDSE PVLKGVKLHYT" gene 25409..26236 /gene="ORF3" CDS 25409..26236 /gene="ORF3" /codon_start=1 /product="ORF3 protein" /protein_id="QHD43417.1" /translation="MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPI QASLPFG WLIVGVALLAVFQSASKIITLKKRWQLALSKGVHFVCNLLLLFVTVYSHL LLVAAGLE APFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTN CYDYCIPY NSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSD YYQLYSTQ LSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPT TTTSVPL" gene 26261..26488 /gene="E" CDS 26261..26488 /gene="E" /note="structural protein; E protein" /codon_start=1 /product="envelope protein" /protein_id="QHD43418.1" /translation="MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTAL RLCAYCC NIVNVSLVKPSFYVYSRVKNLNSSRVPDLLV" gene 26539..27207 /gene="M" CDS 26539..27207 /gene="M" /note="structural protein" /codon_start=1 /product="membrane glycoprotein" /protein_id="QHD43419.1" /translation="MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQF AYANRNR FLYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLS YFIASFRL FARTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIA GHHLGRCD IKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDH SSSSDNIA LLVQ" gene 27218..27403 /gene="ORF6" CDS 27218..27403 /gene="ORF6" /codon_start=1 /product="ORF6 protein" /protein_id="QHD43420.1" /translation="MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLII KNLSKSL TENKYSQLDEEQPMEID" gene 27410..27775 /gene="ORF7" CDS 27410..27775 /gene="ORF7" /codon_start=1 /product="ORF7 protein" /protein_id="QHD43421.1" /translation="MKIILFLALITLATCELYHYQECVRGTTVLLKEPCSS GTYEGNS PFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEVQ ELYSPIFL IVAAIVFITLCFTLKRKTE" gene 27910..28275 /gene="ORF8" CDS 27910..28275 /gene="ORF8" /codon_start=1 /product="ORF8 protein" /protein_id="QHD43422.1" /translation="MKFLVFLGIITTVAAFHQECSLQSCTQHQPYVVDDPC PIHFYSK WYIRVGARKSAPLIELCVDEAGSKSPIQYIDIGNYTVSCLPFTINCQEPK LGSLVVRC SFYEDFLEYHDVRVVLDFI" gene 28290..29549 /gene="N" CDS 28290..29549 /gene="N" /note="structural protein" /codon_start=1 /product="nucleocapsid phosphoprotein" /protein_id="QHD43423.1" /translation="MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARS KQRRPQG LPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRI RGGDGKMK DLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPA NNAAIVLQ LPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARM AGNGGDAA LALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAY NVTQAFGR RGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTP SGTWLTYT AAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQ RQKKQQTV TLLPAADLDDFSKQLQQSMSSADSTQA" 3'UTR 29550..29875 ORIGIN 1 cggtgacgca tacaaaacat tcccaccata ccttcccagg taacaaacca accaactttc 61 gatctcttgt agatctgttc tctaaacgaa ctttaaaatc tgtgtggctg tcactcggct 121 gcatgcttag tgcactcacg cagtataatt aataactaat tactgtcgtt gacaggacac 181 gagtaactcg tctatcttct gcaggctgct tacggtttcg tccgtgttgc agccgatcat 241 cagcacatct aggtttcgtc cgggtgtgac cgaaaggtaa gatggagagc cttgtccctg 301 gtttcaacga gaaaacacac gtccaactca gtttgcctgt tttacaggtt cgcgacgtgc 361 tcgtacgtgg ctttggagac tccgtggagg aggtcttatc agaggcacgt caacatctta 421 aagatggcac ttgtggctta gtagaagttg aaaaaggcgt tttgcctcaa cttgaacagc 481 cctatgtgtt catcaaacgt tcggatgctc gaactgcacc tcatggtcat gttatggttg 541 agctggtagc agaactcgaa ggcattcagt acggtcgtag tggtgagaca cttggtgtcc 601 ttgtccctca tgtgggcgaa ataccagtgg cttaccgcaa ggttcttctt cgtaagaacg 661 gtaataaagg agctggtggc catagttacg gcgccgatct aaagtcattt gacttaggcg 721 acgagcttgg cactgatcct tatgaagatt ttcaagaaaa ctggaacact aaacatagca 781 gtggtgttac ccgtgaactc atgcgtgagc ttaacggagg ggcatacact cgctatgtcg 841 ataacaactt ctgtggccct gatggctacc ctcttgagtg cattaaagac cttctagcac 901 gtgctggtaa agcttcatgc actttgtccg aacaactgga ctttattgac actaagaggg 961 gtgtatactg ctgccgtgaa catgagcatg aaattgcttg gtacacggaa cgttctgaaa 1021 agagctatga attgcagaca ccttttgaaa ttaaattggc aaagaaattt gacaccttca 1081 atggggaatg tccaaatttt gtatttccct taaattccat aatcaagact attcaaccaa 1141 gggttgaaaa gaaaaagctt gatggcttta tgggtagaat tcgatctgtc tatccagttg 1201 cgtcaccaaa tgaatgcaac caaatgtgcc tttcaactct catgaagtgt gatcattgtg 1261 gtgaaacttc atggcagacg ggcgattttg ttaaagccac ttgcgaattt tgtggcactg 1321 agaatttgac taaagaaggt gccactactt gtggttactt accccaaaat gctgttgtta 1381 aaatttattg tccagcatgt cacaattcag aagtaggacc tgagcatagt cttgccgaat 1441 accataatga atctggcttg aaaaccattc ttcgtaaggg tggtcgcact attgcctttg 1501 gaggctgtgt gttctcttat gttggttgcc ataacaagtg tgcctattgg gttccacgtg 1561 ctagcgctaa cataggttgt aaccatacag gtgttgttgg agaaggttcc gaaggtctta 1621 atgacaacct tcttgaaata ctccaaaaag agaaagtcaa catcaatatt gttggtgact 1681 ttaaacttaa tgaagagatc gccattattt tggcatcttt ttctgcttcc acaagtgctt 1741 ttgtggaaac tgtgaaaggt ttggattata aagcattcaa acaaattgtt gaatcctgtg 1801 gtaattttaa agttacaaaa ggaaaagcta aaaaaggtgc ctggaatatt ggtgaacaga 1861 aatcaatact gagtcctctt tatgcatttg catcagaggc tgctcgtgtt gtacgatcaa 1921 ttttctcccg cactcttgaa actgctcaaa attctgtgcg tgttttacag aaggccgcta 1981 taacaatact agatggaatt tcacagtatt cactgagact cattgatgct atgatgttca 2041 catctgattt ggctactaac aatctagttg taatggccta cattacaggt ggtgttgttc 2101 agttgacttc gcagtggcta actaacatct ttggcactgt ttatgaaaaa ctcaaacccg 2161 tccttgattg gcttgaagag aagtttaagg aaggtgtaga gtttcttaga gacggttggg 2221 aaattgttaa atttatctca acctgtgctt gtgaaattgt cggtggacaa attgtcacct 2281 gtgcaaagga aattaaggag agtgttcaga cattctttaa gcttgtaaat aaatttttgg 2341 ctttgtgtgc tgactctatc attattggtg gagctaaact taaagccttg aatttaggtg 2401 aaacatttgt cacgcactca aagggattgt acagaaagtg tgttaaatcc agagaagaaa 2461 ctggcctact catgcctcta aaagccccaa aagaaattat cttcttagag ggagaaacac 2521 ttcccacaga agtgttaaca gaggaagttg tcttgaaaac tggtgattta caaccattag 2581 aacaacctac tagtgaagct gttgaagctc cattggttgg tacaccagtt tgtattaacg 2641 ggcttatgtt gctcgaaatc aaagacacag aaaagtactg tgcccttgca cctaatatga 2701 tggtaacaaa caataccttc acactcaaag gcggtgcacc aacaaaggtt acttttggtg 2761 atgacactgt gatagaagtg caaggttaca agagtgtgaa tatcactttt gaacttgatg 2821 aaaggattga taaagtactt aatgagaagt gctctgccta tacagttgaa ctcggtacag 2881 aagtaaatga gttcgcctgt gttgtggcag atgctgtcat aaaaactttg caaccagtat 2941 ctgaattact tacaccactg ggcattgatt tagatgagtg gagtatggct acatactact 3001 tatttgatga gtctggtgag tttaaattgg cttcacatat gtattgttct ttctaccctc 3061 cagatgagga tgaagaagaa ggtgattgtg aagaagaaga gtttgagcca tcaactcaat 3121 atgagtatgg tactgaagat gattaccaag gtaaaccttt ggaatttggt gccacttctg 3181 ctgctcttca acctgaagaa gagcaagaag aagattggtt agatgatgat agtcaacaaa 3241 ctgttggtca acaagacggc agtgaggaca atcagacaac tactattcaa acaattgttg 3301 aggttcaacc tcaattagag atggaactta caccagttgt tcagactatt gaagtgaata 3361 gttttagtgg ttatttaaaa cttactgaca atgtatacat taaaaatgca gacattgtgg 3421 aagaagctaa aaaggtaaaa ccaacagtgg ttgttaatgc agccaatgtt taccttaaac 3481 atggaggagg tgttgcagga gccttaaata aggctactaa caatgccatg caagttgaat 3541 ctgatgatta catagctact aatggaccac ttaaagtggg tggtagttgt gttttaagcg 3601 gacacaatct tgctaaacac tgtcttcatg ttgtcggccc aaatgttaac aaaggtgaag 3661 acattcaact tcttaagagt gcttatgaaa attttaatca gcacgaagtt ctacttgcac 3721 cattattatc agctggtatt tttggtgctg accctataca ttctttaaga gtttgtgtag 3781 atactgttcg cacaaatgtc tacttagctg tctttgataa aaatctctat gacaaacttg 3841 tttcaagctt tttggaaatg aagagtgaaa agcaagttga acaaaagatc gctgagattc 3901 ctaaagagga agttaagcca tttataactg aaagtaaacc ttcagttgaa cagagaaaac 3961 aagatgataa gaaaatcaaa gcttgtgttg aagaagttac aacaactctg gaagaaacta 4021 agttcctcac agaaaacttg ttactttata ttgacattaa tggcaatctt catccagatt 4081 ctgccactct tgttagtgac attgacatca ctttcttaaa gaaagatgct ccatatatag 4141 tgggtgatgt tgttcaagag ggtgttttaa ctgctgtggt tatacctact aaaaaggctg 4201 gtggcactac tgaaatgcta gcgaaagctt tgagaaaagt gccaacagac aattatataa 4261 ccacttaccc gggtcagggt ttaaatggtt acactgtaga ggaggcaaag acagtgctta 4321 aaaagtgtaa aagtgccttt tacattctac catctattat ctctaatgag aagcaagaaa 4381 ttcttggaac tgtttcttgg aatttgcgag aaatgcttgc acatgcagaa gaaacacgca 4441 aattaatgcc tgtctgtgtg gaaactaaag ccatagtttc aactatacag cgtaaatata 4501 agggtattaa aatacaagag ggtgtggttg attatggtgc tagattttac ttttacacca 4561 gtaaaacaac tgtagcgtca cttatcaaca cacttaacga tctaaatgaa actcttgtta 4621 caatgccact tggctatgta acacatggct taaatttgga agaagctgct cggtatatga 4681 gatctctcaa agtgccagct acagtttctg tttcttcacc tgatgctgtt acagcgtata 4741 atggttatct tacttcttct tctaaaacac ctgaagaaca ttttattgaa accatctcac 4801 ttgctggttc ctataaagat tggtcctatt ctggacaatc tacacaacta ggtatagaat 4861 ttcttaagag aggtgataaa agtgtatatt acactagtaa tcctaccaca ttccacctag 4921 atggtgaagt tatcaccttt gacaatctta agacacttct ttctttgaga gaagtgagga 4981 ctattaaggt gtttacaaca gtagacaaca ttaacctcca cacgcaagtt gtggacatgt 5041 caatgacata tggacaacag tttggtccaa cttatttgga tggagctgat gttactaaaa 5101 taaaacctca taattcacat gaaggtaaaa cattttatgt tttacctaat gatgacactc 5161 tacgtgttga ggcttttgag tactaccaca caactgatcc tagttttctg ggtaggtaca 5221 tgtcagcatt aaatcacact aaaaagtgga aatacccaca agttaatggt ttaacttcta 5281 ttaaatgggc agataacaac tgttatcttg ccactgcatt gttaacactc caacaaatag 5341 agttgaagtt taatccacct gctctacaag atgcttatta cagagcaagg gctggtgaag 5401 ctgctaactt ttgtgcactt atcttagcct actgtaataa gacagtaggt gagttaggtg 5461 atgttagaga aacaatgagt tacttgtttc aacatgccaa tttagattct tgcaaaagag 5521 tcttgaacgt ggtgtgtaaa acttgtggac aacagcagac aacccttaag ggtgtagaag 5581 ctgttatgta catgggcaca ctttcttatg aacaatttaa gaaaggtgtt cagatacctt 5641 gtacgtgtgg taaacaagct acaaaatatc tagtacaaca ggagtcacct tttgttatga 5701 tgtcagcacc acctgctcag tatgaactta agcatggtac atttacttgt gctagtgagt 5761 acactggtaa ttaccagtgt ggtcactata aacatataac ttctaaagaa actttgtatt 5821 gcatagacgg tgctttactt acaaagtcct cagaatacaa aggtcctatt acggatgttt 5881 tctacaaaga aaacagttac acaacaacca taaaaccagt tacttataaa ttggatggtg 5941 ttgtttgtac agaaattgac cctaagttgg acaattatta taagaaagac aattcttatt 6001 tcacagagca accaattgat cttgtaccaa accaaccata tccaaacgca agcttcgata 6061 attttaagtt tgtatgtgat aatatcaaat ttgctgatga tttaaaccag ttaactggtt 6121 ataagaaacc tgcttcaaga gagcttaaag ttacattttt ccctgactta aatggtgatg 6181 tggtggctat tgattataaa cactacacac cctcttttaa gaaaggagct aaattgttac 6241 ataaacctat tgtttggcat gttaacaatg caactaataa agccacgtat aaaccaaata 6301 cctggtgtat acgttgtctt tggagcacaa aaccagttga aacatcaaat tcgtttgatg 6361 tactgaagtc agaggacgcg cagggaatgg ataatcttgc ctgcgaagat ctaaaaccag 6421 tctctgaaga agtagtggaa aatcctacca tacagaaaga cgttcttgag tgtaatgtga 6481 aaactaccga agttgtagga gacattatac ttaaaccagc aaataatagt ttaaaaatta 6541 cagaagaggt tggccacaca gatctaatgg ctgcttatgt agacaattct agtcttacta 6601 ttaagaaacc taatgaatta tctagagtat taggtttgaa aacccttgct actcatggtt 6661 tagctgctgt taatagtgtc ccttgggata ctatagctaa ttatgctaag ccttttctta 6721 acaaagttgt tagtacaact actaacatag ttacacggtg tttaaaccgt gtttgtacta 6781 attatatgcc ttatttcttt actttattgc tacaattgtg tacttttact agaagtacaa 6841 attctagaat taaagcatct atgccgacta ctatagcaaa gaatactgtt aagagtgtcg 6901 gtaaattttg tctagaggct tcatttaatt atttgaagtc acctaatttt tctaaactga 6961 taaatattat aatttggttt ttactattaa gtgtttgcct aggttcttta atctactcaa 7021 ccgctgcttt aggtgtttta atgtctaatt taggcatgcc ttcttactgt actggttaca 7081 gagaaggcta tttgaactct actaatgtca ctattgcaac ctactgtact ggttctatac 7141 cttgtagtgt ttgtcttagt ggtttagatt ctttagacac ctatccttct ttagaaacta 7201 tacaaattac catttcatct tttaaatggg atttaactgc ttttggctta gttgcagagt 7261 ggtttttggc atatattctt ttcactaggt ttttctatgt acttggattg gctgcaatca 7321 tgcaattgtt tttcagctat tttgcagtac attttattag taattcttgg cttatgtggt 7381 taataattaa tcttgtacaa atggccccga tttcagctat ggttagaatg tacatcttct 7441 ttgcatcatt ttattatgta tggaaaagtt atgtgcatgt tgtagacggt tgtaattcat 7501 caacttgtat gatgtgttac aaacgtaata gagcaacaag agtcgaatgt acaactattg 7561 ttaatggtgt tagaaggtcc ttttatgtct atgctaatgg aggtaaaggc ttttgcaaac 7621 tacacaattg gaattgtgtt aattgtgata cattctgtgc tggtagtaca tttattagtg 7681 atgaagttgc gagagacttg tcactacagt ttaaaagacc aataaatcct actgaccagt 7741 cttcttacat cgttgatagt gttacagtga agaatggttc catccatctt tactttgata 7801 aagctggtca aaagacttat gaaagacatt ctctctctca ttttgttaac ttagacaacc 7861 tgagagctaa taacactaaa ggttcattgc ctattaatgt tatagttttt gatggtaaat 7921 caaaatgtga agaatcatct gcaaaatcag cgtctgttta ctacagtcag cttatgtgtc 7981 aacctatact gttactagat caggcattag tgtctgatgt tggtgatagt gcggaagttg 8041 cagttaaaat gtttgatgct tacgttaata cgttttcatc aacttttaac gtaccaatgg 8101 aaaaactcaa aacactagtt gcaactgcag aagctgaact tgcaaagaat gtgtccttag 8161 acaatgtctt atctactttt atttcagcag ctcggcaagg gtttgttgat tcagatgtag 8221 aaactaaaga tgttgttgaa tgtcttaaat tgtcacatca atctgacata gaagttactg 8281 gcgatagttg taataactat atgctcacct ataacaaagt tgaaaacatg acaccccgtg 8341 accttggtgc ttgtattgac tgtagtgcgc gtcatattaa tgcgcaggta gcaaaaagtc 8401 acaacattgc tttgatatgg aacgttaaag atttcatgtc attgtctgaa caactacgaa 8461 aacaaatacg tagtgctgct aaaaagaata acttaccttt taagttgaca tgtgcaacta 8521 ctagacaagt tgttaatgtt gtaacaacaa agatagcact taagggtggt aaaattgtta 8581 ataattggtt gaagcagtta attaaagtta cacttgtgtt cctttttgtt gctgctattt 8641 tctatttaat aacacctgtt catgtcatgt ctaaacatac tgacttttca agtgaaatca 8701 taggatacaa ggctattgat ggtggtgtca ctcgtgacat agcatctaca gatacttgtt 8761 ttgctaacaa acatgctgat tttgacacat ggtttagcca gcgtggtggt agttatacta 8821 atgacaaagc ttgcccattg attgctgcag tcataacaag agaagtgggt tttgtcgtgc 8881 ctggtttgcc tggcacgata ttacgcacaa ctaatggtga ctttttgcat ttcttaccta 8941 gagtttttag tgcagttggt aacatctgtt acacaccatc aaaacttata gagtacactg 9001 actttgcaac atcagcttgt gttttggctg ctgaatgtac aatttttaaa gatgcttctg 9061 gtaagccagt accatattgt tatgatacca atgtactaga aggttctgtt gcttatgaaa 9121 gtttacgccc tgacacacgt tatgtgctca tggatggctc tattattcaa tttcctaaca 9181 cctaccttga aggttctgtt agagtggtaa caacttttga ttctgagtac tgtaggcacg 9241 gcacttgtga aagatcagaa gctggtgttt gtgtatctac tagtggtaga tgggtactta 9301 acaatgatta ttacagatct ttaccaggag ttttctgtgg tgtagatgct gtaaatttac 9361 ttactaatat gtttacacca ctaattcaac ctattggtgc tttggacata tcagcatcta 9421 tagtagctgg tggtattgta gctatcgtag taacatgcct tgcctactat tttatgaggt 9481 ttagaagagc ttttggtgaa tacagtcatg tagttgcctt taatacttta ctattcctta 9541 tgtcattcac tgtactctgt ttaacaccag tttactcatt cttacctggt gtttattctg 9601 ttatttactt gtacttgaca ttttatctta ctaatgatgt ttctttttta gcacatattc 9661 agtggatggt tatgttcaca cctttagtac ctttctggat aacaattgct tatatcattt 9721 gtatttccac aaagcatttc tattggttct ttagtaatta cctaaagaga cgtgtagtct 9781 ttaatggtgt ttcctttagt acttttgaag aagctgcgct gtgcaccttt ttgttaaata 9841 aagaaatgta tctaaagttg cgtagtgatg tgctattacc tcttacgcaa tataatagat 9901 acttagctct ttataataag tacaagtatt ttagtggagc aatggataca actagctaca 9961 gagaagctgc ttgttgtcat ctcgcaaagg ctctcaatga cttcagtaac tcaggttctg 10021 atgttcttta ccaaccacca caaacctcta tcacctcagc tgttttgcag agtggtttta 10081 gaaaaatggc attcccatct ggtaaagttg agggttgtat ggtacaagta acttgtggta 10141 caactacact taacggtctt tggcttgatg acgtagttta ctgtccaaga catgtgatct 10201 gcacctctga agacatgctt aaccctaatt atgaagattt actcattcgt aagtctaatc 10261 ataatttctt ggtacaggct ggtaatgttc aactcagggt tattggacat tctatgcaaa 10321 attgtgtact taagcttaag gttgatacag ccaatcctaa gacacctaag tataagtttg 10381 ttcgcattca accaggacag actttttcag tgttagcttg ttacaatggt tcaccatctg 10441 gtgtttacca atgtgctatg aggcccaatt tcactattaa gggttcattc cttaatggtt 10501 catgtggtag tgttggtttt aacatagatt atgactgtgt ctctttttgt tacatgcacc 10561 atatggaatt accaactgga gttcatgctg gcacagactt agaaggtaac ttttatggac 10621 cttttgttga caggcaaaca gcacaagcag ctggtacgga cacaactatt acagttaatg 10681 ttttagcttg gttgtacgct gctgttataa atggagacag gtggtttctc aatcgattta 10741 ccacaactct taatgacttt aaccttgtgg ctatgaagta caattatgaa cctctaacac 10801 aagaccatgt tgacatacta ggacctcttt ctgctcaaac tggaattgcc gttttagata 10861 tgtgtgcttc attaaaagaa ttactgcaaa atggtatgaa tggacgtacc atattgggta 10921 gtgctttatt agaagatgaa tttacacctt ttgatgttgt tagacaatgc tcaggtgtta 10981 ctttccaaag tgcagtgaaa agaacaatca agggtacaca ccactggttg ttactcacaa 11041 ttttgacttc acttttagtt ttagtccaga gtactcaatg gtctttgttc ttttttttgt 11101 atgaaaatgc ctttttacct tttgctatgg gtattattgc tatgtctgct tttgcaatga 11161 tgtttgtcaa acataagcat gcatttctct gtttgttttt gttaccttct cttgccactg 11221 tagcttattt taatatggtc tatatgcctg ctagttgggt gatgcgtatt atgacatggt 11281 tggatatggt tgatactagt ttgtctggtt ttaagctaaa agactgtgtt atgtatgcat 11341 cagctgtagt gttactaatc cttatgacag caagaactgt gtatgatgat ggtgctagga 11401 gagtgtggac acttatgaat gtcttgacac tcgtttataa agtttattat ggtaatgctt 11461 tagatcaagc catttccatg tgggctctta taatctctgt tacttctaac tactcaggtg 11521 tagttacaac tgtcatgttt ttggccagag gtattgtttt tatgtgtgtt gagtattgcc 11581 ctattttctt cataactggt aatacacttc agtgtataat gctagtttat tgtttcttag 11641 gctatttttg tacttgttac tttggcctct tttgtttact caaccgctac tttagactga 11701 ctcttggtgt ttatgattac ttagtttcta cacaggagtt tagatatatg aattcacagg 11761 gactactccc acccaagaat agcatagatg ccttcaaact caacattaaa ttgttgggtg 11821 ttggtggcaa accttgtatc aaagtagcca ctgtacagtc taaaatgtca gatgtaaagt 11881 gcacatcagt agtcttactc tcagttttgc aacaactcag agtagaatca tcatctaaat 11941 tgtgggctca atgtgtccag ttacacaatg acattctctt agctaaagat actactgaag 12001 cctttgaaaa aatggtttca ctactttctg ttttgctttc catgcagggt gctgtagaca 12061 taaacaagct ttgtgaagaa atgctggaca acagggcaac cttacaagct atagcctcag 12121 agtttagttc ccttccatca tatgcagctt ttgctactgc tcaagaagct tatgagcagg 12181 ctgttgctaa tggtgattct gaagttgttc ttaaaaagtt gaagaagtct ttgaatgtgg 12241 ctaaatctga atttgaccgt gatgcagcca tgcaacgtaa gttggaaaag atggctgatc 12301 aagctatgac ccaaatgtat aaacaggcta gatctgagga caagagggca aaagttacta 12361 gtgctatgca gacaatgctt ttcactatgc ttagaaagtt ggataatgat gcactcaaca 12421 acattatcaa caatgcaaga gatggttgtg ttcccttgaa cataatacct cttacaacag 12481 cagccaaact aatggttgtc ataccagact ataacacata taaaaatacg tgtgatggta 12541 caacatttac ttatgcatca gcattgtggg aaatccaaca ggttgtagat gcagatagta 12601 aaattgttca acttagtgaa attagtatgg acaattcacc taatttagca tggcctctta 12661 ttgtaacagc tttaagggcc aattctgctg tcaaattaca gaataatgag cttagtcctg 12721 ttgcactacg acagatgtct tgtgctgccg gtactacaca aactgcttgc actgatgaca 12781 atgcgttagc ttactacaac acaacaaagg gaggtaggtt tgtacttgca ctgttatccg 12841 atttacagga tttgaaatgg gctagattcc ctaagagtga tggaactggt actatctata 12901 cagaactgga accaccttgt aggtttgtta cagacacacc taaaggtcct aaagtgaagt 12961 atttatactt tattaaagga ttaaacaacc taaatagagg tatggtactt ggtagtttag 13021 ctgccacagt acgtctacaa gctggtaatg caacagaagt gcctgccaat tcaactgtat 13081 tatctttctg tgcttttgct gtagatgctg ctaaagctta caaagattat ctagctagtg 13141 ggggacaacc aatcactaat tgtgttaaga tgttgtgtac acacactggt actggtcagg 13201 caataacagt tacaccggaa gccaatatgg atcaagaatc ctttggtggt gcatcgtgtt 13261 gtctgtactg ccgttgccac atagatcatc caaatcctaa aggattttgt gacttaaaag 13321 gtaagtatgt acaaatacct acaacttgtg ctaatgaccc tgtgggtttt acacttaaaa 13381 acacagtctg taccgtctgc ggtatgtgga aaggttatgg ctgtagttgt gatcaactcc 13441 gcgaacccat gcttcagtca gctgatgcac aatcgttttt aaacgggttt gcggtgtaag 13501 tgcagcccgt cttacaccgt gcggcacagg cactagtact gatgtcgtat acagggcttt 13561 tgacatctac aatgataaag tagctggttt tgctaaattc ctaaaaacta attgttgtcg 13621 cttccaagaa aaggacgaag atgacaattt aattgattct tactttgtag ttaagagaca 13681 cactttctct aactaccaac atgaagaaac aatttataat ttacttaagg attgtccagc 13741 tgttgctaaa catgacttct ttaagtttag aatagacggt gacatggtac cacatatatc 13801 acgtcaacgt cttactaaat acacaatggc agacctcgtc tatgctttaa ggcattttga 13861 tgaaggtaat tgtgacacat taaaagaaat acttgtcaca tacaattgtt gtgatgatga 13921 ttatttcaat aaaaaggact ggtatgattt tgtagaaaac ccagatatat tacgcgtata 13981 cgccaactta ggtgaacgtg tacgccaagc tttgttaaaa acagtacaat tctgtgatgc 14041 catgcgaaat gctggtattg ttggtgtact gacattagat aatcaagatc tcaatggtaa 14101 ctggtatgat ttcggtgatt tcatacaaac cacgccaggt agtggagttc ctgttgtaga 14161 ttcttattat tcattgttaa tgcctatatt aaccttgacc agggctttaa ctgcagagtc 14221 acatgttgac actgacttaa caaagcctta cattaagtgg gatttgttaa aatatgactt 14281 cacggaagag aggttaaaac tctttgaccg ttattttaaa tattgggatc agacatacca 14341 cccaaattgt gttaactgtt tggatgacag atgcattctg cattgtgcaa actttaatgt 14401 tttattctct acagtgttcc cacctacaag ttttggacca ctagtgagaa aaatatttgt 14461 tgatggtgtt ccatttgtag tttcaactgg ataccacttc agagagctag gtgttgtaca 14521 taatcaggat gtaaacttac atagctctag acttagtttt aaggaattac ttgtgtatgc 14581 tgctgaccct gctatgcacg ctgcttctgg taatctatta ctagataaac gcactacgtg 14641 cttttcagta gctgcactta ctaacaatgt tgcttttcaa actgtcaaac ccggtaattt 14701 taacaaagac ttctatgact ttgctgtgtc taagggtttc tttaaggaag gaagttctgt 14761 tgaattaaaa cacttcttct ttgctcagga tggtaatgct gctatcagcg attatgacta 14821 ctatcgttat aatctaccaa caatgtgtga tatcagacaa ctactatttg tagttgaagt 14881 tgttgataag tactttgatt gttacgatgg tggctgtatt aatgctaacc aagtcatcgt 14941 caacaaccta gacaaatcag ctggttttcc atttaataaa tggggtaagg ctagacttta 15001 ttatgattca atgagttatg aggatcaaga tgcacttttc gcatatacaa aacgtaatgt 15061 catccctact ataactcaaa tgaatcttaa gtatgccatt agtgcaaaga atagagctcg 15121 caccgtagct ggtgtctcta tctgtagtac tatgaccaat agacagtttc atcaaaaatt 15181 attgaaatca atagccgcca ctagaggagc tactgtagta attggaacaa gcaaattcta 15241 tggtggttgg cacaacatgt taaaaactgt ttatagtgat gtagaaaacc ctcaccttat 15301 gggttgggat tatcctaaat gtgatagagc catgcctaac atgcttagaa ttatggcctc 15361 acttgttctt gctcgcaaac atacaacgtg ttgtagcttg tcacaccgtt tctatagatt 15421 agctaatgag tgtgctcaag tattgagtga aatggtcatg tgtggcggtt cactatatgt 15481 taaaccaggt ggaacctcat caggagatgc cacaactgct tatgctaata gtgtttttaa 15541 catttgtcaa gctgtcacgg ccaatgttaa tgcactttta tctactgatg gtaacaaaat 15601 tgccgataag tatgtccgca atttacaaca cagactttat gagtgtctct atagaaatag 15661 agatgttgac acagactttg tgaatgagtt ttacgcatat ttgcgtaaac atttctcaat 15721 gatgatactc tctgacgatg ctgttgtgtg tttcaatagc acttatgcat ctcaaggtct 15781 agtggctagc ataaagaact ttaagtcagt tctttattat caaaacaatg tttttatgtc 15841 tgaagcaaaa tgttggactg agactgacct tactaaagga cctcatgaat tttgctctca 15901 acatacaatg ctagttaaac agggtgatga ttatgtgtac cttccttacc cagatccatc 15961 aagaatccta ggggccggct gttttgtaga tgatatcgta aaaacagatg gtacacttat 16021 gattgaacgg ttcgtgtctt tagctataga tgcttaccca cttactaaac atcctaatca 16081 ggagtatgct gatgtctttc atttgtactt acaatacata agaaagctac atgatgagtt 16141 aacaggacac atgttagaca tgtattctgt tatgcttact aatgataaca cttcaaggta 16201 ttgggaacct gagttttatg aggctatgta cacaccgcat acagtcttac aggctgttgg 16261 ggcttgtgtt ctttgcaatt cacagacttc attaagatgt ggtgcttgca tacgtagacc 16321 attcttatgt tgtaaatgct gttacgacca tgtcatatca acatcacata aattagtctt 16381 gtctgttaat ccgtatgttt gcaatgctcc aggttgtgat gtcacagatg tgactcaact 16441 ttacttagga ggtatgagct attattgtaa atcacataaa ccacccatta gttttccatt 16501 gtgtgctaat ggacaagttt ttggtttata taaaaataca tgtgttggta gcgataatgt 16561 tactgacttt aatgcaattg caacatgtga ctggacaaat gctggtgatt acattttagc 16621 taacacctgt actgaaagac tcaagctttt tgcagcagaa acgctcaaag ctactgagga 16681 gacatttaaa ctgtcttatg gtattgctac tgtacgtgaa gtgctgtctg acagagaatt 16741 acatctttca tgggaagttg gtaaacctag accaccactt aaccgaaatt atgtctttac 16801 tggttatcgt gtaactaaaa acagtaaagt acaaatagga gagtacacct ttgaaaaagg 16861 tgactatggt gatgctgttg tttaccgagg tacaacaact tacaaattaa atgttggtga 16921 ttattttgtg ctgacatcac atacagtaat gccattaagt gcacctacac tagtgccaca 16981 agagcactat gttagaatta ctggcttata cccaacactc aatatctcag atgagttttc 17041 tagcaatgtt gcaaattatc aaaaggttgg tatgcaaaag tattctacac tccagggacc 17101 acctggtact ggtaagagtc attttgctat tggcctagct ctctactacc cttctgctcg 17161 catagtgtat acagcttgct ctcatgccgc tgttgatgca ctatgtgaga aggcattaaa 17221 atatttgcct atagataaat gtagtagaat tatacctgca cgtgctcgtg tagagtgttt 17281 tgataaattc aaagtgaatt caacattaga acagtatgtc ttttgtactg taaatgcatt 17341 gcctgagacg acagcagata tagttgtctt tgatgaaatt tcaatggcca caaattatga 17401 tttgagtgtt gtcaatgcca gattacgtgc taagcactat gtgtacattg gcgaccctgc 17461 tcaattacct gcaccacgca cattgctaac taagggcaca ctagaaccag aatatttcaa 17521 ttcagtgtgt agacttatga aaactatagg tccagacatg ttcctcggaa cttgtcggcg 17581 ttgtcctgct gaaattgttg acactgtgag tgctttggtt tatgataata agcttaaagc 17641 acataaagac aaatcagctc aatgctttaa aatgttttat aagggtgtta tcacgcatga 17701 tgtttcatct gcaattaaca ggccacaaat aggcgtggta agagaattcc ttacacgtaa 17761 ccctgcttgg agaaaagctg tctttatttc accttataat tcacagaatg ctgtagcctc 17821 aaagattttg ggactaccaa ctcaaactgt tgattcatca cagggctcag aatatgacta 17881 tgtcatattc actcaaacca ctgaaacagc tcactcttgt aatgtaaaca gatttaatgt 17941 tgctattacc agagcaaaag taggcatact ttgcataatg tctgatagag acctttatga 18001 caagttgcaa tttacaagtc ttgaaattcc acgtaggaat gtggcaactt tacaagctga 18061 aaatgtaaca ggactcttta aagattgtag taaggtaatc actgggttac atcctacaca 18121 ggcacctaca cacctcagtg ttgacactaa attcaaaact gaaggtttat gtgttgacat 18181 acctggcata cctaaggaca tgacctatag aagactcatc tctatgatgg gttttaaaat 18241 gaattatcaa gttaatggtt accctaacat gtttatcacc cgcgaagaag ctataagaca 18301 tgtacgtgca tggattggct tcgatgtcga ggggtgtcat gctactagag aagctgttgg 18361 taccaattta cctttacagc taggtttttc tacaggtgtt aacctagttg ctgtacctac 18421 aggttatgtt gatacaccta ataatacaga tttttccaga gttagtgcta aaccaccgcc 18481 tggagatcaa tttaaacacc tcataccact tatgtacaaa ggacttcctt ggaatgtagt 18541 gcgtataaag attgtacaaa tgttaagtga cacacttaaa aatctctctg acagagtcgt 18601 atttgtctta tgggcacatg gctttgagtt gacatctatg aagtattttg tgaaaatagg 18661 acctgagcgc acctgttgtc tatgtgatag acgtgccaca tgcttttcca ctgcttcaga 18721 cacttatgcc tgttggcatc attctattgg atttgattac gtctataatc cgtttatgat 18781 tgatgttcaa caatggggtt ttacaggtaa cctacaaagc aaccatgatc tgtattgtca 18841 agtccatggt aatgcacatg tagctagttg tgatgcaatc atgactaggt gtctagctgt 18901 ccacgagtgc tttgttaagc gtgttgactg gactattgaa tatcctataa ttggtgatga 18961 actgaagatt aatgcggctt gtagaaaggt tcaacacatg gttgttaaag ctgcattatt 19021 agcagacaaa ttcccagttc ttcacgacat tggtaaccct aaagctatta agtgtgtacc 19081 tcaagctgat gtagaatgga agttctatga tgcacagcct tgtagtgaca aagcttataa 19141 aatagaagaa ttattctatt cttatgccac acattctgac aaattcacag atggtgtatg 19201 cctattttgg aattgcaatg tcgatagata tcctgctaat tccattgttt gtagatttga 19261 cactagagtg ctatctaacc ttaacttgcc tggttgtgat ggtggcagtt tgtatgtaaa 19321 taaacatgca ttccacacac cagcttttga taaaagtgct tttgttaatt taaaacaatt 19381 accatttttc tattactctg acagtccatg tgagtctcat ggaaaacaag tagtgtcaga 19441 tatagattat gtaccactaa agtctgctac gtgtataaca cgttgcaatt taggtggtgc 19501 tgtctgtaga catcatgcta atgagtacag attgtatctc gatgcttata acatgatgat 19561 ctcagctggc tttagcttgt gggtttacaa acaatttgat acttataacc tctggaacac 19621 ttttacaaga cttcagagtt tagaaaatgt ggcttttaat gttgtaaata agggacactt 19681 tgatggacaa cagggtgaag taccagtttc tatcattaat aacactgttt acacaaaagt 19741 tgatggtgtt gatgtagaat tgtttgaaaa taaaacaaca ttacctgtta atgtagcatt 19801 tgagctttgg gctaagcgca acattaaacc agtaccagag gtgaaaatac tcaataattt 19861 gggtgtggac attgctgcta atactgtgat ctgggactac aaaagagatg ctccagcaca 19921 tatatctact attggtgttt gttctatgac tgacatagcc aagaaaccaa ctgaaacgat 19981 ttgtgcacca ctcactgtct tttttgatgg tagagttgat ggtcaagtag acttatttag 20041 aaatgcccgt aatggtgttc ttattacaga aggtagtgtt aaaggtttac aaccatctgt 20101 aggtcccaaa caagctagtc ttaatggagt cacattaatt ggagaagccg taaaaacaca 20161 gttcaattat tataagaaag ttgatggtgt tgtccaacaa ttacctgaaa cttactttac 20221 tcagagtaga aatttacaag aatttaaacc caggagtcaa atggaaattg atttcttaga 20281 attagctatg gatgaattca ttgaacggta taaattagaa ggctatgcct tcgaacatat 20341 cgtttatgga gattttagtc atagtcagtt aggtggttta catctactga ttggactagc 20401 taaacgtttt aaggaatcac cttttgaatt agaagatttt attcctatgg acagtacagt 20461 taaaaactat ttcataacag atgcgcaaac aggttcatct aagtgtgtgt gttctgttat 20521 tgatttatta cttgatgatt ttgttgaaat aataaaatcc caagatttat ctgtagtttc 20581 taaggttgtc aaagtgacta ttgactatac agaaatttca tttatgcttt ggtgtaaaga 20641 tggccatgta gaaacatttt acccaaaatt acaatctagt caagcgtggc aaccgggtgt 20701 tgctatgcct aatctttaca aaatgcaaag aatgctatta gaaaagtgtg accttcaaaa 20761 ttatggtgat agtgcaacat tacctaaagg cataatgatg aatgtcgcaa aatatactca 20821 actgtgtcaa tatttaaaca cattaacatt agctgtaccc tataatatga gagttataca 20881 ttttggtgct ggttctgata aaggagttgc accaggtaca gctgttttaa gacagtggtt 20941 gcctacgggt acgctgcttg tcgattcaga tcttaatgac tttgtctctg atgcagattc 21001 aactttgatt ggtgattgtg caactgtaca tacagctaat aaatgggatc tcattattag 21061 tgatatgtac gaccctaaga ctaaaaatgt tacaaaagaa aatgactcta aagagggttt 21121 tttcacttac atttgtgggt ttatacaaca aaagctagct cttggaggtt ccgtggctat 21181 aaagataaca gaacattctt ggaatgctga tctttataag ctcatgggac acttcgcatg 21241 gtggacagcc tttgttacta atgtgaatgc gtcatcatct gaagcatttt taattggatg 21301 taattatctt ggcaaaccac gcgaacaaat agatggttat gtcatgcatg caaattacat 21361 attttggagg aatacaaatc caattcagtt gtcttcctat tctttatttg acatgagtaa 21421 atttcccctt aaattaaggg gtactgctgt tatgtcttta aaagaaggtc aaatcaatga 21481 tatgatttta tctcttctta gtaaaggtag acttataatt agagaaaaca acagagttgt 21541 tatttctagt gatgttcttg ttaacaacta aacgaacaat gtttgttttt cttgttttat 21601 tgccactagt ctctagtcag tgtgttaatc ttacaaccag aactcaatta ccccctgcat 21661 acactaattc tttcacacgt ggtgtttatt accctgacaa agttttcaga tcctcagttt 21721 tacattcaac tcaggacttg ttcttacctt tcttttccaa tgttacttgg ttccatgcta 21781 tacatgtctc tgggaccaat ggtactaaga ggtttgataa ccctgtccta ccatttaatg 21841 atggtgttta ttttgcttcc actgagaagt ctaacataat aagaggctgg atttttggta 21901 ctactttaga ttcgaagacc cagtccctac ttattgttaa taacgctact aatgttgtta 21961 ttaaagtctg tgaatttcaa ttttgtaatg atccattttt gggtgtttat taccacaaaa 22021 acaacaaaag ttggatggaa agtgagttca gagtttattc tagtgcgaat aattgcactt 22081 ttgaatatgt ctctcagcct tttcttatgg accttgaagg aaaacagggt aatttcaaaa 22141 atcttaggga atttgtgttt aagaatattg atggttattt taaaatatat tctaagcaca 22201 cgcctattaa tttagtgcgt gatctccctc agggtttttc ggctttagaa ccattggtag 22261 atttgccaat aggtattaac atcactaggt ttcaaacttt acttgcttta catagaagtt 22321 atttgactcc tggtgattct tcttcaggtt ggacagctgg tgctgcagct tattatgtgg 22381 gttatcttca acctaggact tttctattaa aatataatga aaatggaacc attacagatg 22441 ctgtagactg tgcacttgac cctctctcag aaacaaagtg tacgttgaaa tccttcactg 22501 tagaaaaagg aatctatcaa acttctaact ttagagtcca accaacagaa tctattgtta 22561 gatttcctaa tattacaaac ttgtgccctt ttggtgaagt ttttaacgcc accagatttg 22621 catctgttta tgcttggaac aggaagagaa tcagcaactg tgttgctgat tattctgtcc 22681 tatataattc cgcatcattt tccactttta agtgttatgg agtgtctcct actaaattaa 22741 atgatctctg ctttactaat gtctatgcag attcatttgt aattagaggt gatgaagtca 22801 gacaaatcgc tccagggcaa actggaaaga ttgctgatta taattataaa ttaccagatg 22861 attttacagg ctgcgttata gcttggaatt ctaacaatct tgattctaag gttggtggta 22921 attataatta cctgtataga ttgtttagga agtctaatct caaacctttt gagagagata 22981 tttcaactga aatctatcag gccggtagca caccttgtaa tggtgttgaa ggttttaatt 23041 gttactttcc tttacaatca tatggtttcc aacccactaa tggtgttggt taccaaccat 23101 acagagtagt agtactttct tttgaacttc tacatgcacc agcaactgtt tgtggaccta 23161 aaaagtctac taatttggtt aaaaacaaat gtgtcaattt caacttcaat ggtttaacag 23221 gcacaggtgt tcttactgag tctaacaaaa agtttctgcc tttccaacaa tttggcagag 23281 acattgctga cactactgat gctgtccgtg atccacagac acttgagatt cttgacatta 23341 caccatgttc ttttggtggt gtcagtgtta taacaccagg aacaaatact tctaaccagg 23401 ttgctgttct ttatcaggat gttaactgca cagaagtccc tgttgctatt catgcagatc 23461 aacttactcc tacttggcgt gtttattcta caggttctaa tgtttttcaa acacgtgcag 23521 gctgtttaat aggggctgaa catgtcaaca actcatatga gtgtgacata cccattggtg 23581 caggtatatg cgctagttat cagactcaga ctaattctcc tcggcgggca cgtagtgtag 23641 ctagtcaatc catcattgcc tacactatgt cacttggtgc agaaaattca gttgcttact 23701 ctaataactc tattgccata cccacaaatt ttactattag tgttaccaca gaaattctac 23761 cagtgtctat gaccaagaca tcagtagatt gtacaatgta catttgtggt gattcaactg 23821 aatgcagcaa tcttttgttg caatatggca gtttttgtac acaattaaac cgtgctttaa 23881 ctggaatagc tgttgaacaa gacaaaaaca cccaagaagt ttttgcacaa gtcaaacaaa 23941 tttacaaaac accaccaatt aaagattttg gtggttttaa tttttcacaa atattaccag 24001 atccatcaaa accaagcaag aggtcattta ttgaagatct acttttcaac aaagtgacac 24061 ttgcagatgc tggcttcatc aaacaatatg gtgattgcct tggtgatatt gctgctagag 24121 acctcatttg tgcacaaaag tttaacggcc ttactgtttt gccacctttg ctcacagatg 24181 aaatgattgc tcaatacact tctgcactgt tagcgggtac aatcacttct ggttggacct 24241 ttggtgcagg tgctgcatta caaataccat ttgctatgca aatggcttat aggtttaatg 24301 gtattggagt tacacagaat gttctctatg agaaccaaaa attgattgcc aaccaattta 24361 atagtgctat tggcaaaatt caagactcac tttcttccac agcaagtgca cttggaaaac 24421 ttcaagatgt ggtcaaccaa aatgcacaag ctttaaacac gcttgttaaa caacttagct 24481 ccaattttgg tgcaatttca agtgttttaa atgatatcct ttcacgtctt gacaaagttg 24541 aggctgaagt gcaaattgat aggttgatca caggcagact tcaaagtttg cagacatatg 24601 tgactcaaca attaattaga gctgcagaaa tcagagcttc tgctaatctt gctgctacta 24661 aaatgtcaga gtgtgtactt ggacaatcaa aaagagttga tttttgtgga aagggctatc 24721 atcttatgtc cttccctcag tcagcacctc atggtgtagt cttcttgcat gtgacttatg 24781 tccctgcaca agaaaagaac ttcacaactg ctcctgccat ttgtcatgat ggaaaagcac 24841 actttcctcg tgaaggtgtc tttgtttcaa atggcacaca ctggtttgta acacaaagga 24901 atttttatga accacaaatc attactacag acaacacatt tgtgtctggt aactgtgatg 24961 ttgtaatagg aattgtcaac aacacagttt atgatccttt gcaacctgaa ttagactcat 25021 tcaaggagga gttagataaa tattttaaga atcatacatc accagatgtt gatttaggtg 25081 acatctctgg cattaatgct tcagttgtaa acattcaaaa agaaattgac cgcctcaatg 25141 aggttgccaa gaatttaaat gaatctctca tcgatctcca agaacttgga aagtatgagc 25201 agtatataaa atggccatgg tacatttggc taggttttat agctggcttg attgccatag 25261 taatggtgac aattatgctt tgctgtatga ccagttgctg tagttgtctc aagggctgtt 25321 gttcttgtgg atcctgctgc aaatttgatg aagacgactc tgagccagtg ctcaaaggag 25381 tcaaattaca ttacacataa acgaacttat ggatttgttt atgagaatct tcacaattgg 25441 aactgtaact ttgaagcaag gtgaaatcaa ggatgctact ccttcagatt ttgttcgcgc 25501 tactgcaacg ataccgatac aagcctcact ccctttcgga tggcttattg ttggcgttgc 25561 acttcttgct gtttttcaga gcgcttccaa aatcataacc ctcaaaaaga gatggcaact 25621 agcactctcc aagggtgttc actttgtttg caacttgctg ttgttgtttg taacagttta 25681 ctcacacctt ttgctcgttg ctgctggcct tgaagcccct tttctctatc tttatgcttt 25741 agtctacttc ttgcagagta taaactttgt aagaataata atgaggcttt ggctttgctg 25801 gaaatgccgt tccaaaaacc cattacttta tgatgccaac tattttcttt gctggcatac 25861 taattgttac gactattgta taccttacaa tagtgtaact tcttcaattg tcattacttc 25921 aggtgatggc acaacaagtc ctatttctga acatgactac cagattggtg gttatactga 25981 aaaatgggaa tctggagtaa aagactgtgt tgtattacac agttacttca cttcagacta 26041 ttaccagctg tactcaactc aattgagtac agacactggt gttgaacatg ttaccttctt 26101 catctacaat aaaattgttg atgagcctga agaacatgtc caaattcaca caatcgacgg 26161 ttcatccgga gttgttaatc cagtaatgga accaatttat gatgaaccga cgacgactac 26221 tagcgtgcct ttgtaagcac aagctgatga gtacgaactt atgtactcat tcgtttcgga 26281 agagacaggt acgttaatag ttaatagcgt acttcttttt cttgctttcg tggtattctt 26341 gctagttaca ctagccatcc ttactgcgct tcgattgtgt gcgtactgct gcaatattgt 26401 taacgtgagt cttgtaaaac cttcttttta cgtttactct cgtgttaaaa atctgaattc 26461 ttctagagtt cctgatcttc tggtctaaac gaactaaata ttatattagt ttttctgttt 26521 ggaactttaa ttttagccat ggcagattcc aacggtacta ttaccgttga agagcttaaa 26581 aagctccttg aacaatggaa cctagtaata ggtttcctat tccttacatg gatttgtctt 26641 ctacaatttg cctatgccaa caggaatagg tttttgtata taattaagtt aattttcctc 26701 tggctgttat ggccagtaac tttagcttgt tttgtgcttg ctgctgttta cagaataaat 26761 tggatcaccg gtggaattgc tatcgcaatg gcttgtcttg taggcttgat gtggctcagc 26821 tacttcattg cttctttcag actgtttgcg cgtacgcgtt ccatgtggtc attcaatcca 26881 gaaactaaca ttcttctcaa cgtgccactc catggcacta ttctgaccag accgcttcta 26941 gaaagtgaac tcgtaatcgg agctgtgatc cttcgtggac atcttcgtat tgctggacac 27001 catctaggac gctgtgacat caaggacctg cctaaagaaa tcactgttgc tacatcacga 27061 acgctttctt attacaaatt gggagcttcg cagcgtgtag caggtgactc aggttttgct 27121 gcatacagtc gctacaggat tggcaactat aaattaaaca cagaccattc cagtagcagt 27181 gacaatattg ctttgcttgt acagtaagtg acaacagatg tttcatctcg ttgactttca 27241 ggttactata gcagagatat tactaattat tatgaggact tttaaagttt ccatttggaa 27301 tcttgattac atcataaacc tcataattaa aaatttatct aagtcactaa ctgagaataa 27361 atattctcaa ttagatgaag agcaaccaat ggagattgat taaacgaaca tgaaaattat 27421 tcttttcttg gcactgataa cactcgctac ttgtgagctt tatcactacc aagagtgtgt 27481 tagaggtaca acagtacttt taaaagaacc ttgctcttct ggaacatacg agggcaattc 27541 accatttcat cctctagctg ataacaaatt tgcactgact tgctttagca ctcaatttgc 27601 ttttgcttgt cctgacggcg taaaacacgt ctatcagtta cgtgccagat cagtttcacc 27661 taaactgttc atcagacaag aggaagttca agaactttac tctccaattt ttcttattgt 27721 tgcggcaata gtgtttataa cactttgctt cacactcaaa agaaagacag aatgattgaa 27781 ctttcattaa ttgacttcta tttgtgcttt ttagcctttc tgctattcct tgttttaatt 27841 atgcttatta tcttttggtt ctcacttgaa ctgcaagatc ataatgaaac ttgtcacgcc 27901 taaacgaaca tgaaatttct tgttttctta ggaatcatca caactgtagc tgcatttcac 27961 caagaatgta gtttacagtc atgtactcaa catcaaccat atgtagttga tgacccgtgt 28021 cctattcact tctattctaa atggtatatt agagtaggag ctagaaaatc agcaccttta 28081 attgaattgt gcgtggatga ggctggttct aaatcaccca ttcagtacat cgatatcggt 28141 aattatacag tttcctgttt accttttaca attaattgcc aggaacctaa attgggtagt 28201 cttgtagtgc gttgttcgtt ctatgaagac tttttagagt atcatgacgt tcgtgttgtt 28261 ttagatttca tctaaacgaa caaactaaaa tgtctgataa tggaccccaa aatcagcgaa 28321 atgcaccccg cattacgttt ggtggaccct cagattcaac tggcagtaac cagaatggag 28381 aacgcagtgg ggcgcgatca aaacaacgtc ggccccaagg tttacccaat aatactgcgt 28441 cttggttcac cgctctcact caacatggca aggaagacct taaattccct cgaggacaag 28501 gcgttccaat taacaccaat agcagtccag atgaccaaat tggctactac cgaagagcta 28561 ccagacgaat tcgtggtggt gacggtaaaa tgaaagatct cagtccaaga tggtatttct 28621 actacctagg aactgggcca gaagctggac ttccctatgg tgctaacaaa gacggcatca 28681 tatgggttgc aactgaggga gccttgaata caccaaaaga tcacattggc acccgcaatc 28741 ctgctaacaa tgctgcaatc gtgctacaac ttcctcaagg aacaacattg ccaaaaggct 28801 tctacgcaga agggagcaga ggcggcagtc aagcctcttc tcgttcctca tcacgtagtc 28861 gcaacagttc aagaaattca actccaggca gcagtagggg aacttctcct gctagaatgg 28921 ctggcaatgg cggtgatgct gctcttgctt tgctgctgct tgacagattg aaccagcttg 28981 agagcaaaat gtctggtaaa ggccaacaac aacaaggcca aactgtcact aagaaatctg 29041 ctgctgaggc ttctaagaag cctcggcaaa aacgtactgc cactaaagca tacaatgtaa 29101 cacaagcttt cggcagacgt ggtccagaac aaacccaagg aaattttggg gaccaggaac 29161 taatcagaca aggaactgat tacaaacatt ggccgcaaat tgcacaattt gcccccagcg 29221 cttcagcgtt cttcggaatg tcgcgcattg gcatggaagt cacaccttcg ggaacgtggt 29281 tgacctacac agctgccatc aaattggatg acaaagatcc aaatttcaaa gatcaagtca 29341 ttttgctgaa taagcatatt gacgcataca aaacattccc accaacagag cctaaaaagg 29401 acaaaaagaa gaaggctgat gaaactcaag ccttaccgca gagacagaag aaacagcaaa 29461 ctgtgactct tcttcctgct gcagatttgg atgatttctc caaacaattg caacaatcca 29521 tgagcagtgc tgactcaact caggcctaaa ctcatgcaga ccacacaagg cagatgggct 29581 atataaacgt tttcgctttt ccgtttacga tatatagtct actcttgtgc agaatgaatt 29641 ctcgtaacta catagcacaa gtagatgtag ttaactttaa tctcacatag caatctttaa 29701 tcagtgtgta acattaggga ggacttgaaa gagccaccac attttcaccg aggccacgcg 29761 gagtacgatc gagtgtacag tgaacaatgc tagggagagc tgcctatatg gaagagccct 29821 aatgtgtaaa attaatttta gtagtgctat ccccatgtga ttttaatagc ttctt //
cancel

 

[gsgs]

http://gctype.wdcm.org/

I didn't yet manage to find something. Seems also not compatible with my old firefox browser in all aspects.

search .... 15,865 entries
species with genomic sequence : 7,659 entries

alphabetical list of species at http://gctype.wdcm.org/species.jsp

walking through 383 pages one by one (you can't select a pagenumber directly ?!)

mainly bacteria

nothing under coronavirus at page 87

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... seeing the other posts now .... hi Gert, how did you find that ?
I'm looking for their list of Bat-Coronaviruses