Shiloh
Editor, Senior Moderator
Source: http://7thspace.com/headlines/41262..._candidate_generated_by_reverse_genetics.html
Evaluation of a genetically modified foot-and-mouth disease virus vaccine candidate generated by reverse genetics
Foot-and-mouth disease (FMD) is the most economically important and highly contagious disease of cloven-hoofed animals worldwide. Control of the disease has been mainly based on large-scale vaccinations with whole-virus inactivated vaccines.
In recent years, a series of outbreaks of type O FMD occurred in China (including Chinese Taipei, Chinese Hong Kong) posed a tremendous threat to Chinese animal husbandry. Its causative agent, type O FMDV, has evolved into three topotypes (East-South Asia (ME-SA), Southeast Asia (SEA), Cathay (CHY)) in these regions, which represents an important obstacle to disease control...
...Conclusions: Some amino acid substitutions in the FMDV vaccine strain genome did not have an effect on the ability of viral replication in vitro. The vaccine prepared from genetically modified FMDV by reverse genetics significantly improved the protective efficacy to the variant of the CHA topotype, compared with the wild O/HN/CHA/93 virus.
Thus, the full-length cDNA clone of FMDV can be a useful tool to develop genetically engineered FMDV vaccine candidates to help control porcinophilic FMD epidemics in China.
Author: Pinghua LiXingwen BaiPu SunDong LiZengjun LuYimei CaoYuanfang FuHuifang BaoYingli ChenBaoxia XieZaixin Liu
Credits/Source: BMC Veterinary Research 2012, 8:57
Evaluation of a genetically modified foot-and-mouth disease virus vaccine candidate generated by reverse genetics
Foot-and-mouth disease (FMD) is the most economically important and highly contagious disease of cloven-hoofed animals worldwide. Control of the disease has been mainly based on large-scale vaccinations with whole-virus inactivated vaccines.
In recent years, a series of outbreaks of type O FMD occurred in China (including Chinese Taipei, Chinese Hong Kong) posed a tremendous threat to Chinese animal husbandry. Its causative agent, type O FMDV, has evolved into three topotypes (East-South Asia (ME-SA), Southeast Asia (SEA), Cathay (CHY)) in these regions, which represents an important obstacle to disease control...
...Conclusions: Some amino acid substitutions in the FMDV vaccine strain genome did not have an effect on the ability of viral replication in vitro. The vaccine prepared from genetically modified FMDV by reverse genetics significantly improved the protective efficacy to the variant of the CHA topotype, compared with the wild O/HN/CHA/93 virus.
Thus, the full-length cDNA clone of FMDV can be a useful tool to develop genetically engineered FMDV vaccine candidates to help control porcinophilic FMD epidemics in China.
Author: Pinghua LiXingwen BaiPu SunDong LiZengjun LuYimei CaoYuanfang FuHuifang BaoYingli ChenBaoxia XieZaixin Liu
Credits/Source: BMC Veterinary Research 2012, 8:57