tetano
Editor, Senior Moderator
Arch Virol. 2018 Sep 5. doi: 10.1007/s00705-018-4016-2. [Epub ahead of print]
[h=1]Chimeric Newcastle disease virus-vectored vaccine protects chickens against H9N2 avian influenza virus in the presence of pre-existing NDV immunity.[/h] Liu J[SUP]1[/SUP], Xue L[SUP]1[/SUP], Hu S[SUP]1,[/SUP][SUP]2[/SUP], Cheng H[SUP]1[/SUP], Deng Y[SUP]1[/SUP], Hu Z[SUP]1[/SUP], Wang X[SUP]1,[/SUP][SUP]2[/SUP], Liu X[SUP]3,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A chimeric Newcastle disease virus (NDV) vector (NDV/AI4-TFHN) was constructed with the replacement of the ectodomains of the fusion and hemagglutinin-neuraminidase proteins by those from avian paramyxovirus type 2. The chimeric virus induced high antibody response in chickens pre-immunized with NDV. A recombinant vaccine candidate, NDV/AI4-TFHN-H9, expressing the hemagglutinin of H9N2 avian influenza virus, was generated, on the basis of the chimeric NDV vector mentioned above. The NDV/AI4-TFHN-H9 vaccine elicited H9-specific hemagglutination inhibition antibodies in chickens pre-immunized with NDV vaccine, and reduced the numbers of chickens shedding virus after H9N2 challenge. NDV/AI4-TFHN-H9 could serve as an alternative vaccine for the prevention of H9N2 infection in commercial poultry flocks.
PMID: 30187143 DOI: 10.1007/s00705-018-4016-2
[h=1]Chimeric Newcastle disease virus-vectored vaccine protects chickens against H9N2 avian influenza virus in the presence of pre-existing NDV immunity.[/h] Liu J[SUP]1[/SUP], Xue L[SUP]1[/SUP], Hu S[SUP]1,[/SUP][SUP]2[/SUP], Cheng H[SUP]1[/SUP], Deng Y[SUP]1[/SUP], Hu Z[SUP]1[/SUP], Wang X[SUP]1,[/SUP][SUP]2[/SUP], Liu X[SUP]3,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A chimeric Newcastle disease virus (NDV) vector (NDV/AI4-TFHN) was constructed with the replacement of the ectodomains of the fusion and hemagglutinin-neuraminidase proteins by those from avian paramyxovirus type 2. The chimeric virus induced high antibody response in chickens pre-immunized with NDV. A recombinant vaccine candidate, NDV/AI4-TFHN-H9, expressing the hemagglutinin of H9N2 avian influenza virus, was generated, on the basis of the chimeric NDV vector mentioned above. The NDV/AI4-TFHN-H9 vaccine elicited H9-specific hemagglutination inhibition antibodies in chickens pre-immunized with NDV vaccine, and reduced the numbers of chickens shedding virus after H9N2 challenge. NDV/AI4-TFHN-H9 could serve as an alternative vaccine for the prevention of H9N2 infection in commercial poultry flocks.
PMID: 30187143 DOI: 10.1007/s00705-018-4016-2