tetano
Editor, Senior Moderator
J Gen Virol. 2014 Dec 12. pii: jgv.0.000025. doi: 10.1099/jgv.0.000025. [Epub ahead of print]
[h=1]Chimeric NA and mutant PB1 gene constellation improves growth and yield of H5N1 vaccine candidate virus.[/h] Plant EP[SUP]1[/SUP], Ye Z[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We previously showed that a mutated PB1 gene improved the growth kinetics of a H3N2 influenza reassortant. Here we show that the same mutations improve the growth kinetics of a virus containing the A/VietNam/1203/2004 (H5N1) HA and NA. Total protein yield and neuraminidase activity were increased when a chimeric NA was included. These increases indicate that the synergistic effect is due to the gene constellation containing both the altered PB1 gene and the chimeric NA gene.
PMID: 25502649 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25502649
[h=1]Chimeric NA and mutant PB1 gene constellation improves growth and yield of H5N1 vaccine candidate virus.[/h] Plant EP[SUP]1[/SUP], Ye Z[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We previously showed that a mutated PB1 gene improved the growth kinetics of a H3N2 influenza reassortant. Here we show that the same mutations improve the growth kinetics of a virus containing the A/VietNam/1203/2004 (H5N1) HA and NA. Total protein yield and neuraminidase activity were increased when a chimeric NA was included. These increases indicate that the synergistic effect is due to the gene constellation containing both the altered PB1 gene and the chimeric NA gene.
PMID: 25502649 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25502649