tetano
Editor, Senior Moderator
J Virol. 2013 Sep 25. [Epub ahead of print]
Chicken IFITM3 restricts Influenza viruses and Lyssaviruses in vitro.
Smith SE, Gibson MS, Wash RS, Ferrara F, Wright E, Temperton N, Kellam P, Fife M.
Source
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK.
Abstract
Interferon inducible transmembrane protein 3 (IFITM3) is an effector protein of the innate immune system. It confers potent, cell intrinsic resistance to infection by diverse enveloped viruses both in vitro and in vivo, including influenza viruses, West Nile virus and dengue virus. IFITM3 prevents cytosolic entry of these viruses by blocking complete virus envelope fusion with cell endosome membranes. Although the IFITM locus, which includes IFITM1, 2, 3 and 5, is present in mammalian species, this locus has not been unambiguously identified or functionally characterised in avian species. Here we show that the IFITM locus exists in chickens and is syntenic with the IFITM locus in mammals. The chicken IFITM3 protein restricts cell infection by influenza A viruses and lyssaviruses to a similar level as its human orthologue. Furthermore, we show that chicken IFITM3 is functional in chicken cells and that knock-down of constitutive expression in chicken fibroblasts results in enhanced infection by influenza A virus. Chicken IFITM2 and 3 are constitutively expressed in all tissues examined, whereas IFITM1 is only expressed in the bursa of Fabricius, gastrointestinal tract, caecal tonsil and trachea. Despite being highly divergent at the amino acid level, IFITM3 proteins of birds and mammals can restrict replication of viruses that are able to infect different host species, suggesting IFITM proteins may provide a crucial barrier for zoonotic infections.
PMID:
24067955
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24067955
Chicken IFITM3 restricts Influenza viruses and Lyssaviruses in vitro.
Smith SE, Gibson MS, Wash RS, Ferrara F, Wright E, Temperton N, Kellam P, Fife M.
Source
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK.
Abstract
Interferon inducible transmembrane protein 3 (IFITM3) is an effector protein of the innate immune system. It confers potent, cell intrinsic resistance to infection by diverse enveloped viruses both in vitro and in vivo, including influenza viruses, West Nile virus and dengue virus. IFITM3 prevents cytosolic entry of these viruses by blocking complete virus envelope fusion with cell endosome membranes. Although the IFITM locus, which includes IFITM1, 2, 3 and 5, is present in mammalian species, this locus has not been unambiguously identified or functionally characterised in avian species. Here we show that the IFITM locus exists in chickens and is syntenic with the IFITM locus in mammals. The chicken IFITM3 protein restricts cell infection by influenza A viruses and lyssaviruses to a similar level as its human orthologue. Furthermore, we show that chicken IFITM3 is functional in chicken cells and that knock-down of constitutive expression in chicken fibroblasts results in enhanced infection by influenza A virus. Chicken IFITM2 and 3 are constitutively expressed in all tissues examined, whereas IFITM1 is only expressed in the bursa of Fabricius, gastrointestinal tract, caecal tonsil and trachea. Despite being highly divergent at the amino acid level, IFITM3 proteins of birds and mammals can restrict replication of viruses that are able to infect different host species, suggesting IFITM proteins may provide a crucial barrier for zoonotic infections.
PMID:
24067955
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24067955