tetano
Editor, Senior Moderator
Dev Comp Immunol. 2012 Nov 22. pii: S0145-305X(12)00236-4. doi: 10.1016/j.dci.2012.10.011. [Epub ahead of print]
Chicken dendritic cells are susceptible to highly pathogenic avian influenza viruses which induce strong cytokine responses.
Vervelde L, Reemers SS, van Haarlem DA, Post J, Claassen E, Rebel JM, Jansen CA.
Source
Department of Infectious Diseases and Immunology, Faculty Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands. Electronic address: l.vervelde@uu.nl.
Abstract
Infection with highly pathogenic avian influenza (HPAI) in birds and mammals is associated with severe pathology and increased mortality. We hypothesize that in contrast to low pathogenicity avian influenza (LPAI) infection, HPAI infection of chicken dendritic cells (DC) induces a cytokine deregulation which may contribute to their highly pathogenic nature. Infection of DC with LPAI H7N1 and H5N2 resulted in viral RNA and NP expression without increase in time, in contrast to HPAI H7N1 and H5N2 mRNA expression. No increase in IFN mRNA was detected after infection with LPAI, but after LPAI H5N2, and not LPAI H7N1, infection the level of bioactive IFNα/β significantly increased. After HPAI H7N1 and H5N2 infection, significant increases in IL-8, IFN-α, IFN-γ mRNA expression and in TLR1, 3, and 21 mRNA were observed. This enhanced activation of DC after HPAI infection may trigger deregulation of the immune response as seen during HPAI infection in chickens.
Copyright ? 2012. Published by Elsevier Ltd.
PMID:
23178410
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23178410
Chicken dendritic cells are susceptible to highly pathogenic avian influenza viruses which induce strong cytokine responses.
Vervelde L, Reemers SS, van Haarlem DA, Post J, Claassen E, Rebel JM, Jansen CA.
Source
Department of Infectious Diseases and Immunology, Faculty Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands. Electronic address: l.vervelde@uu.nl.
Abstract
Infection with highly pathogenic avian influenza (HPAI) in birds and mammals is associated with severe pathology and increased mortality. We hypothesize that in contrast to low pathogenicity avian influenza (LPAI) infection, HPAI infection of chicken dendritic cells (DC) induces a cytokine deregulation which may contribute to their highly pathogenic nature. Infection of DC with LPAI H7N1 and H5N2 resulted in viral RNA and NP expression without increase in time, in contrast to HPAI H7N1 and H5N2 mRNA expression. No increase in IFN mRNA was detected after infection with LPAI, but after LPAI H5N2, and not LPAI H7N1, infection the level of bioactive IFNα/β significantly increased. After HPAI H7N1 and H5N2 infection, significant increases in IL-8, IFN-α, IFN-γ mRNA expression and in TLR1, 3, and 21 mRNA were observed. This enhanced activation of DC after HPAI infection may trigger deregulation of the immune response as seen during HPAI infection in chickens.
Copyright ? 2012. Published by Elsevier Ltd.
PMID:
23178410
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23178410