tetano
Editor, Senior Moderator
Virusdisease. 2014;25(3):372-5. doi: 10.1007/s13337-013-0190-8. Epub 2014 Jan 19.
[h=1]Characterization of influenza virus among influenza like illness cases in Mumbai, India.[/h] Roy S[SUP]1[/SUP], Dahake R[SUP]1[/SUP], Patil D[SUP]1[/SUP], Tawde S[SUP]1[/SUP], Mukherjee S[SUP]1[/SUP], Athlekar S[SUP]1[/SUP], Chowdhary A[SUP]1[/SUP], Deshmukh R[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The present study was carried out to monitor influenza viruses by identifying the virus and studying the seasonal variation during 2007-2009 in Mumbai. A total of 193 clinical respiratory samples (nasal and throat swab) were collected from patients having influenza like illness in Mumbai region. One-step real-time reverse-transcriptase PCR (rRTPCR) was used to detect Influenza type A (H1 and H3) and Influenza type B virus. Isolation of the virus was carried out using in vitro system which was further confirmed and typed by hemagglutination assay and hemagglutination inhibition assay. Out of 193 samples 24 (12.4 3%) samples tested positive for influenza virus, of which 13 (6.73 %) were influenza type A virus and 10 (5.18 %) were influenza type B virus, while 1 sample (0.51 %) was positive for both. By culture methods, 3 (1.55 %) viral isolates were obtained. All the three isolates were found to be Influenza type B/Malaysia (Victoria lineage) by Hemagglutination Inhibition Assay. The data generated from the present study reveals that both Influenza type A and B are prevalent in Mumbai with considerable activity. The peak activity was observed during monsoon season.
[h=4]KEYWORDS:[/h] ILI cases; Influenza virus; Phylogeny; rRT PCR
PMID: 25674606 [PubMed] PMCID: PMC4188195 Free PMC Article
[h=1]Characterization of influenza virus among influenza like illness cases in Mumbai, India.[/h] Roy S[SUP]1[/SUP], Dahake R[SUP]1[/SUP], Patil D[SUP]1[/SUP], Tawde S[SUP]1[/SUP], Mukherjee S[SUP]1[/SUP], Athlekar S[SUP]1[/SUP], Chowdhary A[SUP]1[/SUP], Deshmukh R[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The present study was carried out to monitor influenza viruses by identifying the virus and studying the seasonal variation during 2007-2009 in Mumbai. A total of 193 clinical respiratory samples (nasal and throat swab) were collected from patients having influenza like illness in Mumbai region. One-step real-time reverse-transcriptase PCR (rRTPCR) was used to detect Influenza type A (H1 and H3) and Influenza type B virus. Isolation of the virus was carried out using in vitro system which was further confirmed and typed by hemagglutination assay and hemagglutination inhibition assay. Out of 193 samples 24 (12.4 3%) samples tested positive for influenza virus, of which 13 (6.73 %) were influenza type A virus and 10 (5.18 %) were influenza type B virus, while 1 sample (0.51 %) was positive for both. By culture methods, 3 (1.55 %) viral isolates were obtained. All the three isolates were found to be Influenza type B/Malaysia (Victoria lineage) by Hemagglutination Inhibition Assay. The data generated from the present study reveals that both Influenza type A and B are prevalent in Mumbai with considerable activity. The peak activity was observed during monsoon season.
[h=4]KEYWORDS:[/h] ILI cases; Influenza virus; Phylogeny; rRT PCR
PMID: 25674606 [PubMed] PMCID: PMC4188195 Free PMC Article