tetano
Editor, Senior Moderator
Clin Microbiol Infect. 2019 Jun 20. pii: S1198-743X(19)30338-6. doi: 10.1016/j.cmi.2019.06.013. [Epub ahead of print]
[h=1]Characterization of antibody and memory T cell response in H7N9 survivors: A cross-sectional analysis.[/h] Ma MJ[SUP]1[/SUP], Wang XX[SUP]2[/SUP], Wu MN[SUP]3[/SUP], Wang XJ[SUP]4[/SUP], Bao CJ[SUP]5[/SUP], Zhang HJ[SUP]6[/SUP], Yang Y[SUP]7[/SUP], Xu K[SUP]5[/SUP], Wang GL[SUP]8[/SUP], Zhao M[SUP]9[/SUP], Cheng W[SUP]2[/SUP], Chen WJ[SUP]3[/SUP], Zhang WH[SUP]8[/SUP], Fang LQ[SUP]8[/SUP], Liu WJ[SUP]10[/SUP], Chen EF[SUP]11[/SUP], Cao WC[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVES:[/h] Despite the importance of immunological memory for protective immunity against viral infection, whether H7N9-specific antibodies and memory T-cell responses remain detectable years after the original infection is unknown.
[h=4]METHODS:[/h] A cross-sectional study was conducted to investigate the immune memory responses of H7N9 patients who contracted the disease and survived during the 2013-2016 epidemics in China. Sustainability of antibodies and T-cell memory to H7N9 virus were examined. Healthy subjects receiving routine medical examination in physical examination center were recruited as control.
[h=4]RESULTS:[/h] A total of 75 survivors were enrolled and classified into four groups based on the time elapsed from illness onset to specimen collection: three months (n=14), 14 months (n=14), 26 months (n=28), and 36 months (n=19). Approximately 36 months after infection, the geometric mean titers of virus-specific antibodies were significantly lower than titers in patients of three months after infection, but 16 of 19 (84.2%) survivors in the 36-month interval had microneutralization (MN) titer > 40. Despite the overall declining trend, the percentages of virus-specific cytokines-secreting memory CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cells remained higher in survivors at nearly all time points in comparison with control subjects. Linear regression analysis showed that severe disease (mean titer ratio 2.77, 95%CI 1.17-6.49) was associated with higher hemagglutination inhibition (HI) titer, and female sex for both HI (1.92, 1.02-3.57) and MN (3.33, 1.26-9.09) antibody, whereas female sex (mean percentage ratio 1.69, 95%CI 1.08-2.63), underlying medical conditions (1.94, 95%CI 1.09-3.46), and lack of antiviral therapy (2.08, 95%CI 1.04-4.17) were predictors for higher T-cell responses.
[h=4]CONCLUSIONS:[/h] Survivors from H7N9 virus infection produced long-term antibodies and memory T-cells responses. Our findings warrant further serological investigation in general and high-risk populations and have important implications for vaccine design and development.
Copyright ? 2019 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Antibodies; H7N9; Immune Memory; Influenza; Survivors; T-Cells
PMID: 31229595 DOI: 10.1016/j.cmi.2019.06.013
[h=1]Characterization of antibody and memory T cell response in H7N9 survivors: A cross-sectional analysis.[/h] Ma MJ[SUP]1[/SUP], Wang XX[SUP]2[/SUP], Wu MN[SUP]3[/SUP], Wang XJ[SUP]4[/SUP], Bao CJ[SUP]5[/SUP], Zhang HJ[SUP]6[/SUP], Yang Y[SUP]7[/SUP], Xu K[SUP]5[/SUP], Wang GL[SUP]8[/SUP], Zhao M[SUP]9[/SUP], Cheng W[SUP]2[/SUP], Chen WJ[SUP]3[/SUP], Zhang WH[SUP]8[/SUP], Fang LQ[SUP]8[/SUP], Liu WJ[SUP]10[/SUP], Chen EF[SUP]11[/SUP], Cao WC[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVES:[/h] Despite the importance of immunological memory for protective immunity against viral infection, whether H7N9-specific antibodies and memory T-cell responses remain detectable years after the original infection is unknown.
[h=4]METHODS:[/h] A cross-sectional study was conducted to investigate the immune memory responses of H7N9 patients who contracted the disease and survived during the 2013-2016 epidemics in China. Sustainability of antibodies and T-cell memory to H7N9 virus were examined. Healthy subjects receiving routine medical examination in physical examination center were recruited as control.
[h=4]RESULTS:[/h] A total of 75 survivors were enrolled and classified into four groups based on the time elapsed from illness onset to specimen collection: three months (n=14), 14 months (n=14), 26 months (n=28), and 36 months (n=19). Approximately 36 months after infection, the geometric mean titers of virus-specific antibodies were significantly lower than titers in patients of three months after infection, but 16 of 19 (84.2%) survivors in the 36-month interval had microneutralization (MN) titer > 40. Despite the overall declining trend, the percentages of virus-specific cytokines-secreting memory CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cells remained higher in survivors at nearly all time points in comparison with control subjects. Linear regression analysis showed that severe disease (mean titer ratio 2.77, 95%CI 1.17-6.49) was associated with higher hemagglutination inhibition (HI) titer, and female sex for both HI (1.92, 1.02-3.57) and MN (3.33, 1.26-9.09) antibody, whereas female sex (mean percentage ratio 1.69, 95%CI 1.08-2.63), underlying medical conditions (1.94, 95%CI 1.09-3.46), and lack of antiviral therapy (2.08, 95%CI 1.04-4.17) were predictors for higher T-cell responses.
[h=4]CONCLUSIONS:[/h] Survivors from H7N9 virus infection produced long-term antibodies and memory T-cells responses. Our findings warrant further serological investigation in general and high-risk populations and have important implications for vaccine design and development.
Copyright ? 2019 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Antibodies; H7N9; Immune Memory; Influenza; Survivors; T-Cells
PMID: 31229595 DOI: 10.1016/j.cmi.2019.06.013