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Molecular characterization of the surface glycoprotein genes of an H5N1 influenza virus isolated from a human in Guangdong, China
.-J. Zhou<SUP>1</SUP>, J. Fu<SUP>1</SUP>, D.-Y. Fang<SUP>1</SUP>, H.-J. Yan<SUP>1</SUP>, J. Tian<SUP>1</SUP>, J.-M. Zhou<SUP>1</SUP>, J.-P. Tao<SUP>1</SUP>, Y. Liang<SUP>1</SUP> and L.-F. Jiang<SUP>1</SUP><TABLE><TBODY><TR vAlign=top><TD>(1) </TD><TD>Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China</TD></TR></TBODY></TABLE>Received: 31 January 2007 Accepted: 18 April 2007 Published online: 29 May 2007
Summary In March 2006, a human H5N1-infected case was found in Guangdong province, China. Here, we molecularly characterized the hemagglutinin (HA) and neuraminidase (NA) genes of the A/China/GD01/06 (GD01) strain causing the infection. The phylogenetic analyses suggested that the HA and NA genes of GD01 and recent human H5N1 viruses from different provinces of China were probably derived from a common ancestor and the H5N1 human infection was acquired directly from affected poultry. At the cleavage site of HA, GD01 contained multiple basic amino acids, a feature characteristic of highly pathogenic avian influenza A viruses. The virus possessed Gln222, Gly224, Ser223, Asn182, Gln192 residues adjacent to the receptor-binding site, preferential for recognizing SAα2, 3Gal. In addition, the GD01 NA amino acid sequence possessed Asn344 and Phe466, which might be related to the low-pH stability of the sialidase activity and gastrointestinal symptoms of the patient.
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Molecular characterization of the surface glycoprotein genes of an H5N1 influenza virus isolated from a human in Guangdong, China
.-J. Zhou<SUP>1</SUP>, J. Fu<SUP>1</SUP>, D.-Y. Fang<SUP>1</SUP>, H.-J. Yan<SUP>1</SUP>, J. Tian<SUP>1</SUP>, J.-M. Zhou<SUP>1</SUP>, J.-P. Tao<SUP>1</SUP>, Y. Liang<SUP>1</SUP> and L.-F. Jiang<SUP>1</SUP><TABLE><TBODY><TR vAlign=top><TD>(1) </TD><TD>Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China</TD></TR></TBODY></TABLE>Received: 31 January 2007 Accepted: 18 April 2007 Published online: 29 May 2007
Summary In March 2006, a human H5N1-infected case was found in Guangdong province, China. Here, we molecularly characterized the hemagglutinin (HA) and neuraminidase (NA) genes of the A/China/GD01/06 (GD01) strain causing the infection. The phylogenetic analyses suggested that the HA and NA genes of GD01 and recent human H5N1 viruses from different provinces of China were probably derived from a common ancestor and the H5N1 human infection was acquired directly from affected poultry. At the cleavage site of HA, GD01 contained multiple basic amino acids, a feature characteristic of highly pathogenic avian influenza A viruses. The virus possessed Gln222, Gly224, Ser223, Asn182, Gln192 residues adjacent to the receptor-binding site, preferential for recognizing SAα2, 3Gal. In addition, the GD01 NA amino acid sequence possessed Asn344 and Phe466, which might be related to the low-pH stability of the sialidase activity and gastrointestinal symptoms of the patient.
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