tetano
Editor, Senior Moderator
J Gen Virol. 2012 Jun 27. [Epub ahead of print]
Characterization of a porcine intestinal epithelial cell line for influenza virus production.
Sun Z, Huber V, McCormick K, Kaushik RS, Boon AC, Zhu L, Hause B, Webby RJ, Fang Y.
Source
South Dakota State University;
Abstract
We have developed a porcine intestine epithelial cell line, designated SD-PJEC for propagation of influenza viruses. The SD-PJEC cell line is a subclone of the IPEC-J2 cell line, which was originally derived from newborn piglet jejunum (Berschneider, 1989, Gastroenterology, 96: A41). Our results demonstrate that SD-PJEC is a cell line of epithelial origin that preferentially expresses receptors of oligosaccharides with Sia2-6Gal modification. This cell line is permissive to infection with human and swine influenza A viruses and some avian influenza viruses, but poorly support the growth of human-origin influenza B viruses. Propagation of swine-origin influenza viruses in these cells results in a rapid growth rate within the first 24 hours post-infection, and the titers ranged from 4-8 log10 TCID50/ml. The SD-PJEC was further tested as a potential alternative cell line to Madin-Darby canine kidney (MDCK) cells in conjunction with 293T cells for rescue of swine-origin influenza viruses using the reverse genetics system. The recombinant viruses A/swine/North Carolina/18161/02 (H1N1) and A/swine/Texas/4199-2/98 (H3N2) were rescued with virus titers of 7 and 8.25 log10 TCID50/ml, respectively. The availability of this swine specific cell line represents a more relevant substrate for studies and growth of swine-origin influenza viruses.
PMID:
22739061
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22739061
Characterization of a porcine intestinal epithelial cell line for influenza virus production.
Sun Z, Huber V, McCormick K, Kaushik RS, Boon AC, Zhu L, Hause B, Webby RJ, Fang Y.
Source
South Dakota State University;
Abstract
We have developed a porcine intestine epithelial cell line, designated SD-PJEC for propagation of influenza viruses. The SD-PJEC cell line is a subclone of the IPEC-J2 cell line, which was originally derived from newborn piglet jejunum (Berschneider, 1989, Gastroenterology, 96: A41). Our results demonstrate that SD-PJEC is a cell line of epithelial origin that preferentially expresses receptors of oligosaccharides with Sia2-6Gal modification. This cell line is permissive to infection with human and swine influenza A viruses and some avian influenza viruses, but poorly support the growth of human-origin influenza B viruses. Propagation of swine-origin influenza viruses in these cells results in a rapid growth rate within the first 24 hours post-infection, and the titers ranged from 4-8 log10 TCID50/ml. The SD-PJEC was further tested as a potential alternative cell line to Madin-Darby canine kidney (MDCK) cells in conjunction with 293T cells for rescue of swine-origin influenza viruses using the reverse genetics system. The recombinant viruses A/swine/North Carolina/18161/02 (H1N1) and A/swine/Texas/4199-2/98 (H3N2) were rescued with virus titers of 7 and 8.25 log10 TCID50/ml, respectively. The availability of this swine specific cell line represents a more relevant substrate for studies and growth of swine-origin influenza viruses.
PMID:
22739061
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22739061