tetano
Editor, Senior Moderator
Virol J. 2019 Dec 21;16(1):162. doi: 10.1186/s12985-019-1271-0. [h=1]Changes in RNA secondary structure affect NS1 protein expression during early stage influenza virus infection.[/h]
Baranovskaya I[SUP]1,[/SUP][SUP]2[/SUP], Sergeeva M[SUP]3,[/SUP][SUP]4[/SUP], Fadeev A[SUP]3[/SUP], Kadirova R[SUP]3[/SUP], Ivanova A[SUP]3[/SUP], Ramsay E[SUP]3[/SUP], Vasin A[SUP]3,[/SUP][SUP]5,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Smorodintsev Research Institute of Influenza, 15/17 Prof. Popova Str, Saint Petersburg, 197376, Russia. irina.baranovskaja.1992@gmail.com. 2 Peter the Great St. Petersburg Polytechnic University, 29 Polytechnicheskaya Str, Saint Petersburg, 195251, Russia. irina.baranovskaja.1992@gmail.com. 3 Smorodintsev Research Institute of Influenza, 15/17 Prof. Popova Str, Saint Petersburg, 197376, Russia. 4 Global Viral Network, 725 West Lombard St Room S413, Baltimore, MD, 21201, USA. 5 Peter the Great St. Petersburg Polytechnic University, 29 Polytechnicheskaya Str, Saint Petersburg, 195251, Russia.
[h=3]Abstract[/h] RNA secondary structures play a key role in splicing, gene expression, microRNA biogenesis, RNA editing, and other biological processes. The importance of RNA structures has been demonstrated in the life cycle of RNA-containing viruses, including the influenza virus. At least two regions of conserved secondary structure in NS segment (+) RNA are predicted to vary among influenza virus strains with respect to thermodynamic stability; both fall in the NS1 open reading frame. The NS1 protein is involved in multiple virus-host interaction processes, and its main function is to inhibit the cellular immune response to viral infection. Using a reverse genetics approach, four influenza virus strains were constructed featuring mutations that have different effects on RNA secondary structure. Growth curve experiments and ELISA data show that, at least in the first viral replication cycle, mutations G123A and A132G affecting RNA structure in the (82-148) NS RNA region influence NS1 protein expression.
[h=4]KEYWORDS:[/h] Influenza virus; NS gene; NS1 protein; RNA hairpin; RNA secondary structure; Reverse genetics
PMID: 31864377 DOI: 10.1186/s12985-019-1271-0
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Baranovskaya I[SUP]1,[/SUP][SUP]2[/SUP], Sergeeva M[SUP]3,[/SUP][SUP]4[/SUP], Fadeev A[SUP]3[/SUP], Kadirova R[SUP]3[/SUP], Ivanova A[SUP]3[/SUP], Ramsay E[SUP]3[/SUP], Vasin A[SUP]3,[/SUP][SUP]5,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Smorodintsev Research Institute of Influenza, 15/17 Prof. Popova Str, Saint Petersburg, 197376, Russia. irina.baranovskaja.1992@gmail.com. 2 Peter the Great St. Petersburg Polytechnic University, 29 Polytechnicheskaya Str, Saint Petersburg, 195251, Russia. irina.baranovskaja.1992@gmail.com. 3 Smorodintsev Research Institute of Influenza, 15/17 Prof. Popova Str, Saint Petersburg, 197376, Russia. 4 Global Viral Network, 725 West Lombard St Room S413, Baltimore, MD, 21201, USA. 5 Peter the Great St. Petersburg Polytechnic University, 29 Polytechnicheskaya Str, Saint Petersburg, 195251, Russia.
[h=3]Abstract[/h] RNA secondary structures play a key role in splicing, gene expression, microRNA biogenesis, RNA editing, and other biological processes. The importance of RNA structures has been demonstrated in the life cycle of RNA-containing viruses, including the influenza virus. At least two regions of conserved secondary structure in NS segment (+) RNA are predicted to vary among influenza virus strains with respect to thermodynamic stability; both fall in the NS1 open reading frame. The NS1 protein is involved in multiple virus-host interaction processes, and its main function is to inhibit the cellular immune response to viral infection. Using a reverse genetics approach, four influenza virus strains were constructed featuring mutations that have different effects on RNA secondary structure. Growth curve experiments and ELISA data show that, at least in the first viral replication cycle, mutations G123A and A132G affecting RNA structure in the (82-148) NS RNA region influence NS1 protein expression.
[h=4]KEYWORDS:[/h] Influenza virus; NS gene; NS1 protein; RNA hairpin; RNA secondary structure; Reverse genetics
PMID: 31864377 DOI: 10.1186/s12985-019-1271-0
Free full text