tetano
Editor, Senior Moderator
J Clin Microbiol. 2015 Feb 18. pii: JCM.03236-14. [Epub ahead of print]
[h=1]Challenges in the antigenic characterization of circulating influenza A(H3N2) viruses during the 2011-12 influenza season: an ongoing problem?[/h] Kossyvakis A[SUP]1[/SUP], Pogka V[SUP]1[/SUP], Melidou A[SUP]2[/SUP], Moutousi A[SUP]1[/SUP], Gioula G[SUP]2[/SUP], Kalliaropoulos A[SUP]1[/SUP], Exindari M[SUP]2[/SUP], Emmanouil M[SUP]1[/SUP], Horefti E[SUP]1[/SUP], Spala G[SUP]3[/SUP], Meijer A[SUP]4[/SUP], Malisiovas N[SUP]2[/SUP], Mentis AF[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Genetic and antigenic characterization of 37 representative influenza A(H3N2) strains isolated in Greece during the 2011-12 winter season was performed to evaluate matching of the viruses with the seasonal influenza A/Perth/16/2009 vaccine strain. Haemagglutinin-gene sequence analysis revealed that all Greek strains clustered within the Victoria/208 genetic clade. Furthermore, mutations in the antigenic and glycosylation sites suggested potential antigenic drift. Our haemagglutination-inhibition (HI) analysis showed that the Greek viruses were Perth/16-like, however, these viruses were characterised as Victoria/208-like when tested at the UK WHO Collaborating Centre (WHOCC) by HI in the presence of oseltamivir, a finding consistent with the genetic characterization data. Variability in the HI testing performance experienced by other European laboratories indicated that A(H3N2) virus antigenic analysis has limitations and until its standardization, national influenza reference laboratories should include genetic characterization results for selecting representative viruses for detailed antigenic analysis by WHOCC.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25694524 [PubMed - as supplied by publisher]
[h=1]Challenges in the antigenic characterization of circulating influenza A(H3N2) viruses during the 2011-12 influenza season: an ongoing problem?[/h] Kossyvakis A[SUP]1[/SUP], Pogka V[SUP]1[/SUP], Melidou A[SUP]2[/SUP], Moutousi A[SUP]1[/SUP], Gioula G[SUP]2[/SUP], Kalliaropoulos A[SUP]1[/SUP], Exindari M[SUP]2[/SUP], Emmanouil M[SUP]1[/SUP], Horefti E[SUP]1[/SUP], Spala G[SUP]3[/SUP], Meijer A[SUP]4[/SUP], Malisiovas N[SUP]2[/SUP], Mentis AF[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Genetic and antigenic characterization of 37 representative influenza A(H3N2) strains isolated in Greece during the 2011-12 winter season was performed to evaluate matching of the viruses with the seasonal influenza A/Perth/16/2009 vaccine strain. Haemagglutinin-gene sequence analysis revealed that all Greek strains clustered within the Victoria/208 genetic clade. Furthermore, mutations in the antigenic and glycosylation sites suggested potential antigenic drift. Our haemagglutination-inhibition (HI) analysis showed that the Greek viruses were Perth/16-like, however, these viruses were characterised as Victoria/208-like when tested at the UK WHO Collaborating Centre (WHOCC) by HI in the presence of oseltamivir, a finding consistent with the genetic characterization data. Variability in the HI testing performance experienced by other European laboratories indicated that A(H3N2) virus antigenic analysis has limitations and until its standardization, national influenza reference laboratories should include genetic characterization results for selecting representative viruses for detailed antigenic analysis by WHOCC.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25694524 [PubMed - as supplied by publisher]