tetano
Editor, Senior Moderator
Cells
. 2021 Sep 12;10(9):2393.
doi: 10.3390/cells10092393.
Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2
Jae Hyun Park[SUP] 1 [/SUP], Yuri Choi[SUP] 1 [/SUP], Chul-Woo Lim[SUP] 1 [/SUP], Ji-Min Park[SUP] 1 [/SUP], Shin-Hye Yu[SUP] 2 [/SUP], Yujin Kim[SUP] 2 [/SUP], Hae Jung Han[SUP] 3 [/SUP], Chun-Hyung Kim[SUP] 2 [/SUP], Young-Sook Song[SUP] 1 [/SUP], Chul Kim[SUP] 1 [/SUP], Seung Rok Yu[SUP] 4 [/SUP], Eun Young Oh[SUP] 4 [/SUP], Sang-Myeong Lee[SUP] 5 [/SUP], Jisook Moon[SUP] 1 [/SUP]
Affiliations
Abstract
Extracellular vesicles (EVs) are cell-released, nanometer-scaled, membrane-bound materials and contain diverse contents including proteins, small peptides, and nucleic acids. Once released, EVs can alter the microenvironment and regulate a myriad of cellular physiology components, including cell-cell communication, proliferation, differentiation, and immune responses against viral infection. Among the cargoes in the vesicles, small non-coding micro-RNAs (miRNAs) have received attention in that they can regulate the expression of a variety of human genes as well as external viral genes via binding to the complementary mRNAs. In this study, we tested the potential of EVs as therapeutic agents for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. First, we found that the mesenchymal stem-cell-derived EVs (MSC-EVs) enabled the rescue of the cytopathic effect of SARS-CoV-2 virus and the suppression of proinflammatory responses in the infected cells by inhibiting the viral replication. We found that these anti-viral responses were mediated by 17 miRNAs matching the rarely mutated, conserved 3'-untranslated regions (UTR) of the viral genome. The top five miRNAs highly expressed in the MSC-EVs, miR-92a-3p, miR-26a-5p, miR-23a-3p, miR-103a-3p, and miR-181a-5p, were tested. They were bound to the complemented sequence which led to the recovery of the cytopathic effects. These findings suggest that the MSC-EVs are a potential candidate for multiple variants of anti-SARS-CoV-2.
Keywords: COVID-19; SARS-CoV-2; cytokine storm; extracellular vesicle; mesenchymal stem cell; miRNA.
. 2021 Sep 12;10(9):2393.
doi: 10.3390/cells10092393.
Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2
Jae Hyun Park[SUP] 1 [/SUP], Yuri Choi[SUP] 1 [/SUP], Chul-Woo Lim[SUP] 1 [/SUP], Ji-Min Park[SUP] 1 [/SUP], Shin-Hye Yu[SUP] 2 [/SUP], Yujin Kim[SUP] 2 [/SUP], Hae Jung Han[SUP] 3 [/SUP], Chun-Hyung Kim[SUP] 2 [/SUP], Young-Sook Song[SUP] 1 [/SUP], Chul Kim[SUP] 1 [/SUP], Seung Rok Yu[SUP] 4 [/SUP], Eun Young Oh[SUP] 4 [/SUP], Sang-Myeong Lee[SUP] 5 [/SUP], Jisook Moon[SUP] 1 [/SUP]
Affiliations
- PMID: 34572043
- DOI: 10.3390/cells10092393
Abstract
Extracellular vesicles (EVs) are cell-released, nanometer-scaled, membrane-bound materials and contain diverse contents including proteins, small peptides, and nucleic acids. Once released, EVs can alter the microenvironment and regulate a myriad of cellular physiology components, including cell-cell communication, proliferation, differentiation, and immune responses against viral infection. Among the cargoes in the vesicles, small non-coding micro-RNAs (miRNAs) have received attention in that they can regulate the expression of a variety of human genes as well as external viral genes via binding to the complementary mRNAs. In this study, we tested the potential of EVs as therapeutic agents for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. First, we found that the mesenchymal stem-cell-derived EVs (MSC-EVs) enabled the rescue of the cytopathic effect of SARS-CoV-2 virus and the suppression of proinflammatory responses in the infected cells by inhibiting the viral replication. We found that these anti-viral responses were mediated by 17 miRNAs matching the rarely mutated, conserved 3'-untranslated regions (UTR) of the viral genome. The top five miRNAs highly expressed in the MSC-EVs, miR-92a-3p, miR-26a-5p, miR-23a-3p, miR-103a-3p, and miR-181a-5p, were tested. They were bound to the complemented sequence which led to the recovery of the cytopathic effects. These findings suggest that the MSC-EVs are a potential candidate for multiple variants of anti-SARS-CoV-2.
Keywords: COVID-19; SARS-CoV-2; cytokine storm; extracellular vesicle; mesenchymal stem cell; miRNA.