tetano
Editor, Senior Moderator
Cells
. 2022 Aug 18;11(16):2572.
doi: 10.3390/cells11162572.
Impact of Microbiota Depletion by Antibiotics on SARS-CoV-2 Infection of K18-hACE2 Mice
Patrícia Brito Rodrigues[SUP] 1 [/SUP], Giovanni Freitas Gomes[SUP] 2 [/SUP], Monara K S C Angelim[SUP] 3 [/SUP], Gabriela F Souza[SUP] 4 [/SUP], Stefanie Primon Muraro[SUP] 4 [/SUP], Daniel A Toledo-Teixeira[SUP] 4 [/SUP], Bruna Amanda Cruz Rattis[SUP] 5 [/SUP], Amanda Stephane Passos[SUP] 2 6 [/SUP], Laís Passarielo Pral[SUP] 1 [/SUP], Vinícius de Rezende Rodovalho[SUP] 1 [/SUP], Arilson Bernardo Dos Santos P Gomes[SUP] 1 [/SUP], Valquíria Aparecida Matheus[SUP] 1 [/SUP], André Saraiva Leão Marcelo Antunes[SUP] 7 [/SUP], Fernanda Crunfli[SUP] 7 [/SUP], Krist Helen Antunes[SUP] 8 [/SUP], Ana Paula Duarte de Souza[SUP] 8 [/SUP], Sílvio Roberto Consonni[SUP] 9 [/SUP], Luiz Osório Leiria[SUP] 2 6 [/SUP], José Carlos Alves-Filho[SUP] 2 6 [/SUP], Thiago M Cunha[SUP] 2 6 [/SUP], Pedro M M Moraes-Vieira[SUP] 3 10 11 [/SUP], José Luiz Proença-Módena[SUP] 4 11 [/SUP], Marco Aurélio R Vinolo[SUP] 1 10 11 [/SUP]
Affiliations
Abstract
Clinical and experimental data indicate that severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infection is associated with significant changes in the composition and function of intestinal microbiota. However, the relevance of these effects for SARS-CoV-2 pathophysiology is unknown. In this study, we analyzed the impact of microbiota depletion after antibiotic treatment on the clinical and immunological responses of K18-hACE2 mice to SARS-CoV-2 infection. Mice were treated with a combination of antibiotics (kanamycin, gentamicin, metronidazole, vancomycin, and colistin, Abx) for 3 days, and 24 h later, they were infected with SARS-CoV-2 B lineage. Here, we show that more than 80% of mice succumbed to infection by day 11 post-infection. Treatment with Abx had no impact on mortality. However, Abx-treated mice presented better clinical symptoms, with similar weight loss between infected-treated and non-treated groups. We observed no differences in lung and colon histopathological scores or lung, colon, heart, brain and kidney viral load between groups on day 5 of infection. Despite some minor differences in the expression of antiviral and inflammatory markers in the lungs and colon, no robust change was observed in Abx-treated mice. Together, these findings indicate that microbiota depletion has no impact on SARS-CoV-2 infection in mice.
Keywords: COVID-19; SARS-CoV-2; colon; gut-to-lung axis; intestinal microbiota; respiratory infection.
. 2022 Aug 18;11(16):2572.
doi: 10.3390/cells11162572.
Impact of Microbiota Depletion by Antibiotics on SARS-CoV-2 Infection of K18-hACE2 Mice
Patrícia Brito Rodrigues[SUP] 1 [/SUP], Giovanni Freitas Gomes[SUP] 2 [/SUP], Monara K S C Angelim[SUP] 3 [/SUP], Gabriela F Souza[SUP] 4 [/SUP], Stefanie Primon Muraro[SUP] 4 [/SUP], Daniel A Toledo-Teixeira[SUP] 4 [/SUP], Bruna Amanda Cruz Rattis[SUP] 5 [/SUP], Amanda Stephane Passos[SUP] 2 6 [/SUP], Laís Passarielo Pral[SUP] 1 [/SUP], Vinícius de Rezende Rodovalho[SUP] 1 [/SUP], Arilson Bernardo Dos Santos P Gomes[SUP] 1 [/SUP], Valquíria Aparecida Matheus[SUP] 1 [/SUP], André Saraiva Leão Marcelo Antunes[SUP] 7 [/SUP], Fernanda Crunfli[SUP] 7 [/SUP], Krist Helen Antunes[SUP] 8 [/SUP], Ana Paula Duarte de Souza[SUP] 8 [/SUP], Sílvio Roberto Consonni[SUP] 9 [/SUP], Luiz Osório Leiria[SUP] 2 6 [/SUP], José Carlos Alves-Filho[SUP] 2 6 [/SUP], Thiago M Cunha[SUP] 2 6 [/SUP], Pedro M M Moraes-Vieira[SUP] 3 10 11 [/SUP], José Luiz Proença-Módena[SUP] 4 11 [/SUP], Marco Aurélio R Vinolo[SUP] 1 10 11 [/SUP]
Affiliations
- PMID: 36010648
- DOI: 10.3390/cells11162572
Abstract
Clinical and experimental data indicate that severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infection is associated with significant changes in the composition and function of intestinal microbiota. However, the relevance of these effects for SARS-CoV-2 pathophysiology is unknown. In this study, we analyzed the impact of microbiota depletion after antibiotic treatment on the clinical and immunological responses of K18-hACE2 mice to SARS-CoV-2 infection. Mice were treated with a combination of antibiotics (kanamycin, gentamicin, metronidazole, vancomycin, and colistin, Abx) for 3 days, and 24 h later, they were infected with SARS-CoV-2 B lineage. Here, we show that more than 80% of mice succumbed to infection by day 11 post-infection. Treatment with Abx had no impact on mortality. However, Abx-treated mice presented better clinical symptoms, with similar weight loss between infected-treated and non-treated groups. We observed no differences in lung and colon histopathological scores or lung, colon, heart, brain and kidney viral load between groups on day 5 of infection. Despite some minor differences in the expression of antiviral and inflammatory markers in the lungs and colon, no robust change was observed in Abx-treated mice. Together, these findings indicate that microbiota depletion has no impact on SARS-CoV-2 infection in mice.
Keywords: COVID-19; SARS-CoV-2; colon; gut-to-lung axis; intestinal microbiota; respiratory infection.