tetano
Editor, Senior Moderator
Cells
. 2023 Oct 21;12(20):2501.
doi: 10.3390/cells12202501. Differential Transcriptomic Signatures of Small Airway Cell Cultures Derived from IPF and COVID-19-Induced Exacerbation of Interstitial Lung Disease
Katie Uhl[SUP] 1 [/SUP], Shreya Paithankar[SUP] 1 [/SUP], Dmitry Leshchiner[SUP] 1 [/SUP], Tara E Jager[SUP] 2 [/SUP], Mohamed Abdelgied[SUP] 1 [/SUP], Bhavna Dixit[SUP] 1 [/SUP], Raya Marashdeh[SUP] 1 [/SUP], Dewen Luo-Li[SUP] 1 [/SUP], Kaylie Tripp[SUP] 1 [/SUP], Angela M Peraino[SUP] 2 [/SUP], Maximiliano Tamae Kakazu[SUP] 2 [/SUP], Cameron Lawson[SUP] 2 [/SUP], Dave W Chesla[SUP] 2 [/SUP], Ningzhi Luo-Li[SUP] 1 [/SUP], Edward T Murphy[SUP] 2 3 [/SUP], Jeremy Prokop[SUP] 1 4 [/SUP], Bin Chen[SUP] 1 4 [/SUP], Reda E Girgis[SUP] 2 [/SUP], Xiaopeng Li[SUP] 1 [/SUP]
Affiliations
Idiopathic pulmonary fibrosis (IPF) is a pathological condition wherein lung injury precipitates the deposition of scar tissue, ultimately leading to a decline in pulmonary function. Existing research indicates a notable exacerbation in the clinical prognosis of IPF patients following infection with COVID-19. This investigation employed bulk RNA-sequencing methodologies to describe the transcriptomic profiles of small airway cell cultures derived from IPF and post-COVID fibrosis patients. Differential gene expression analysis unveiled heightened activation of pathways associated with microtubule assembly and interferon signaling in IPF cell cultures. Conversely, post-COVID fibrosis cell cultures exhibited distinctive characteristics, including the upregulation of pathways linked to extracellular matrix remodeling, immune system response, and TGF-β1 signaling. Notably, BMP signaling levels were elevated in cell cultures derived from IPF patients compared to non-IPF control and post-COVID fibrosis samples. These findings underscore the molecular distinctions between IPF and post-COVID fibrosis, particularly in the context of signaling pathways associated with each condition. A better understanding of the underlying molecular mechanisms holds the promise of identifying potential therapeutic targets for future interventions in these diseases.
Keywords: COVID-19; ILD; IPF; RNA-sequencing; TGF-β1; coronavirus; idiopathic pulmonary fibrosis; interstitial lung disease.
. 2023 Oct 21;12(20):2501.
doi: 10.3390/cells12202501. Differential Transcriptomic Signatures of Small Airway Cell Cultures Derived from IPF and COVID-19-Induced Exacerbation of Interstitial Lung Disease
Katie Uhl[SUP] 1 [/SUP], Shreya Paithankar[SUP] 1 [/SUP], Dmitry Leshchiner[SUP] 1 [/SUP], Tara E Jager[SUP] 2 [/SUP], Mohamed Abdelgied[SUP] 1 [/SUP], Bhavna Dixit[SUP] 1 [/SUP], Raya Marashdeh[SUP] 1 [/SUP], Dewen Luo-Li[SUP] 1 [/SUP], Kaylie Tripp[SUP] 1 [/SUP], Angela M Peraino[SUP] 2 [/SUP], Maximiliano Tamae Kakazu[SUP] 2 [/SUP], Cameron Lawson[SUP] 2 [/SUP], Dave W Chesla[SUP] 2 [/SUP], Ningzhi Luo-Li[SUP] 1 [/SUP], Edward T Murphy[SUP] 2 3 [/SUP], Jeremy Prokop[SUP] 1 4 [/SUP], Bin Chen[SUP] 1 4 [/SUP], Reda E Girgis[SUP] 2 [/SUP], Xiaopeng Li[SUP] 1 [/SUP]
Affiliations
- PMID: 37887346
- DOI: 10.3390/cells12202501
Idiopathic pulmonary fibrosis (IPF) is a pathological condition wherein lung injury precipitates the deposition of scar tissue, ultimately leading to a decline in pulmonary function. Existing research indicates a notable exacerbation in the clinical prognosis of IPF patients following infection with COVID-19. This investigation employed bulk RNA-sequencing methodologies to describe the transcriptomic profiles of small airway cell cultures derived from IPF and post-COVID fibrosis patients. Differential gene expression analysis unveiled heightened activation of pathways associated with microtubule assembly and interferon signaling in IPF cell cultures. Conversely, post-COVID fibrosis cell cultures exhibited distinctive characteristics, including the upregulation of pathways linked to extracellular matrix remodeling, immune system response, and TGF-β1 signaling. Notably, BMP signaling levels were elevated in cell cultures derived from IPF patients compared to non-IPF control and post-COVID fibrosis samples. These findings underscore the molecular distinctions between IPF and post-COVID fibrosis, particularly in the context of signaling pathways associated with each condition. A better understanding of the underlying molecular mechanisms holds the promise of identifying potential therapeutic targets for future interventions in these diseases.
Keywords: COVID-19; ILD; IPF; RNA-sequencing; TGF-β1; coronavirus; idiopathic pulmonary fibrosis; interstitial lung disease.