tetano
Editor, Senior Moderator
Cell
. 2020 Apr 18;S0092-8674(20)30406-2.
doi: 10.1016/j.cell.2020.04.011. Online ahead of print.
The Architecture of SARS-CoV-2 Transcriptome
Dongwan Kim[SUP] 1 [/SUP], Joo-Yeon Lee[SUP] 2 [/SUP], Jeong-Sun Yang[SUP] 2 [/SUP], Jun Won Kim[SUP] 2 [/SUP], V Narry Kim[SUP] 3 [/SUP], Hyeshik Chang[SUP] 4 [/SUP]
Affiliations
Abstract
SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3' tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.
Keywords: COVID-19; RNA modification; SARS-CoV-2; coronavirus; direct RNA sequencing; discontinuous transcription; epitranscriptome; nanopore; poly(A) tail; transcriptome.
Copyright ? 2020 Elsevier Inc. All rights reserved.
. 2020 Apr 18;S0092-8674(20)30406-2.
doi: 10.1016/j.cell.2020.04.011. Online ahead of print.
The Architecture of SARS-CoV-2 Transcriptome
Dongwan Kim[SUP] 1 [/SUP], Joo-Yeon Lee[SUP] 2 [/SUP], Jeong-Sun Yang[SUP] 2 [/SUP], Jun Won Kim[SUP] 2 [/SUP], V Narry Kim[SUP] 3 [/SUP], Hyeshik Chang[SUP] 4 [/SUP]
Affiliations
- PMID: 32330414
- DOI: 10.1016/j.cell.2020.04.011
Abstract
SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3' tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.
Keywords: COVID-19; RNA modification; SARS-CoV-2; coronavirus; direct RNA sequencing; discontinuous transcription; epitranscriptome; nanopore; poly(A) tail; transcriptome.
Copyright ? 2020 Elsevier Inc. All rights reserved.