tetano
Editor, Senior Moderator
Cell
. 2021 Jun 8;S0092-8674(21)00706-6.
doi: 10.1016/j.cell.2021.06.005. Online ahead of print.
SARS-CoV-2 mRNA vaccination induces functionally diverse antibodies to NTD, RBD, and S2
Fatima Amanat[SUP] 1 [/SUP], Mahima Thapa[SUP] 2 [/SUP], Tinting Lei[SUP] 2 [/SUP], Shaza M Sayed Ahmed[SUP] 2 [/SUP], Daniel C Adelsberg[SUP] 3 [/SUP], Juan Manuel Carreño[SUP] 3 [/SUP], Shirin Strohmeier[SUP] 3 [/SUP], Aaron J Schmitz[SUP] 2 [/SUP], Sarah Zafar[SUP] 3 [/SUP], Julian Q Zhou[SUP] 4 [/SUP], Willemijn Rijnink[SUP] 3 [/SUP], Hala Alshammary[SUP] 3 [/SUP], Nicholas Borcherding[SUP] 2 [/SUP], Ana Gonzalez Reiche[SUP] 5 [/SUP], Komal Srivastava[SUP] 3 [/SUP], Emilia Mia Sordillo[SUP] 6 [/SUP], Harm van Bakel[SUP] 7 [/SUP], Personalized Virology Initiative; Jackson S Turner[SUP] 2 [/SUP], Goran Bajic[SUP] 8 [/SUP], Viviana Simon[SUP] 9 [/SUP], Ali H Ellebedy[SUP] 10 [/SUP], Florian Krammer[SUP] 11 [/SUP]
Collaborators, Affiliations
Abstract
In this study we profiled vaccine-induced polyclonal antibodies as well as plasmablast-derived mAbs from individuals who received SARS-CoV-2 spike mRNA vaccine. Polyclonal antibody responses in vaccinees were robust and comparable to or exceeded those seen after natural infection. However, the ratio of binding to neutralizing antibodies after vaccination was greater than that after natural infection and, at the monoclonal level, we found that the majority of vaccine-induced antibodies did not have neutralizing activity. We also found a co-dominance of mAbs targeting the NTD and RBD of SARS-CoV-2 spike and an original antigenic-sin like backboost to spikes of seasonal human coronaviruses OC43 and HKU1. Neutralizing activity of NTD mAbs but not RBD mAbs against a clinical viral isolate carrying E484K as well as extensive changes in the NTD was abolished, suggesting that a proportion of vaccine-induced RBD binding antibodies may provide substantial protection against viral variants carrying single E484K RBD mutations.
Keywords: NTD; RBD; SARS-CoV-2; mAbs; mRNA vaccination; plasmablasts; spike.
. 2021 Jun 8;S0092-8674(21)00706-6.
doi: 10.1016/j.cell.2021.06.005. Online ahead of print.
SARS-CoV-2 mRNA vaccination induces functionally diverse antibodies to NTD, RBD, and S2
Fatima Amanat[SUP] 1 [/SUP], Mahima Thapa[SUP] 2 [/SUP], Tinting Lei[SUP] 2 [/SUP], Shaza M Sayed Ahmed[SUP] 2 [/SUP], Daniel C Adelsberg[SUP] 3 [/SUP], Juan Manuel Carreño[SUP] 3 [/SUP], Shirin Strohmeier[SUP] 3 [/SUP], Aaron J Schmitz[SUP] 2 [/SUP], Sarah Zafar[SUP] 3 [/SUP], Julian Q Zhou[SUP] 4 [/SUP], Willemijn Rijnink[SUP] 3 [/SUP], Hala Alshammary[SUP] 3 [/SUP], Nicholas Borcherding[SUP] 2 [/SUP], Ana Gonzalez Reiche[SUP] 5 [/SUP], Komal Srivastava[SUP] 3 [/SUP], Emilia Mia Sordillo[SUP] 6 [/SUP], Harm van Bakel[SUP] 7 [/SUP], Personalized Virology Initiative; Jackson S Turner[SUP] 2 [/SUP], Goran Bajic[SUP] 8 [/SUP], Viviana Simon[SUP] 9 [/SUP], Ali H Ellebedy[SUP] 10 [/SUP], Florian Krammer[SUP] 11 [/SUP]
Collaborators, Affiliations
- PMID: 34192529
- DOI: 10.1016/j.cell.2021.06.005
Abstract
In this study we profiled vaccine-induced polyclonal antibodies as well as plasmablast-derived mAbs from individuals who received SARS-CoV-2 spike mRNA vaccine. Polyclonal antibody responses in vaccinees were robust and comparable to or exceeded those seen after natural infection. However, the ratio of binding to neutralizing antibodies after vaccination was greater than that after natural infection and, at the monoclonal level, we found that the majority of vaccine-induced antibodies did not have neutralizing activity. We also found a co-dominance of mAbs targeting the NTD and RBD of SARS-CoV-2 spike and an original antigenic-sin like backboost to spikes of seasonal human coronaviruses OC43 and HKU1. Neutralizing activity of NTD mAbs but not RBD mAbs against a clinical viral isolate carrying E484K as well as extensive changes in the NTD was abolished, suggesting that a proportion of vaccine-induced RBD binding antibodies may provide substantial protection against viral variants carrying single E484K RBD mutations.
Keywords: NTD; RBD; SARS-CoV-2; mAbs; mRNA vaccination; plasmablasts; spike.