tetano
Editor, Senior Moderator
Cell Rep
. 2022 Nov 8;41(6):111613.
doi: 10.1016/j.celrep.2022.111613.
The memory B cell response to influenza vaccination is impaired in older persons
Alice R Burton[SUP] 1 [/SUP], Stephane M Guillaume[SUP] 1 [/SUP], William S Foster[SUP] 1 [/SUP], Adam K Wheatley[SUP] 2 [/SUP], Danika L Hill[SUP] 3 [/SUP], Edward J Carr[SUP] 4 [/SUP], Michelle A Linterman[SUP] 5 [/SUP]
Affiliations
Abstract
Influenza infection imparts an age-related increase in mortality and morbidity. The most effective countermeasure is vaccination; however, vaccines offer modest protection in older adults. To investigate how aging impacts the memory B cell response, we track hemagglutinin-specific B cells by indexed flow sorting and single-cell RNA sequencing (scRNA-seq) in 20 healthy adults that were administered the trivalent influenza vaccine. We demonstrate age-related skewing in the memory B cell compartment 6 weeks after vaccination, with younger adults developing hemagglutinin-specific memory B cells with an FcRL5[SUP]+[/SUP] "atypical" phenotype, showing evidence of somatic hypermutation and positive selection, which happened to a lesser extent in older persons. We use publicly available scRNA-seq from paired human lymph node and blood samples to corroborate that FcRL5[SUP]+[/SUP] atypical memory B cells can derive from germinal center (GC) precursors. Together, this study shows that the aged human GC reaction and memory B cell response following vaccination is defective.
Keywords: B cell; CP: Immunology; aging; antibody selection; influenza; memory; vaccination.
. 2022 Nov 8;41(6):111613.
doi: 10.1016/j.celrep.2022.111613.
The memory B cell response to influenza vaccination is impaired in older persons
Alice R Burton[SUP] 1 [/SUP], Stephane M Guillaume[SUP] 1 [/SUP], William S Foster[SUP] 1 [/SUP], Adam K Wheatley[SUP] 2 [/SUP], Danika L Hill[SUP] 3 [/SUP], Edward J Carr[SUP] 4 [/SUP], Michelle A Linterman[SUP] 5 [/SUP]
Affiliations
- PMID: 36351385
- DOI: 10.1016/j.celrep.2022.111613
Abstract
Influenza infection imparts an age-related increase in mortality and morbidity. The most effective countermeasure is vaccination; however, vaccines offer modest protection in older adults. To investigate how aging impacts the memory B cell response, we track hemagglutinin-specific B cells by indexed flow sorting and single-cell RNA sequencing (scRNA-seq) in 20 healthy adults that were administered the trivalent influenza vaccine. We demonstrate age-related skewing in the memory B cell compartment 6 weeks after vaccination, with younger adults developing hemagglutinin-specific memory B cells with an FcRL5[SUP]+[/SUP] "atypical" phenotype, showing evidence of somatic hypermutation and positive selection, which happened to a lesser extent in older persons. We use publicly available scRNA-seq from paired human lymph node and blood samples to corroborate that FcRL5[SUP]+[/SUP] atypical memory B cells can derive from germinal center (GC) precursors. Together, this study shows that the aged human GC reaction and memory B cell response following vaccination is defective.
Keywords: B cell; CP: Immunology; aging; antibody selection; influenza; memory; vaccination.