tetano
Editor, Senior Moderator
Cell Rep
. 2022 Jun 13;111013.
doi: 10.1016/j.celrep.2022.111013. Online ahead of print.
Temporal associations of B and T cell immunity with robust vaccine responsiveness in a 16-week interval BNT162b2 regimen
Manon Nayrac[SUP] 1 [/SUP], Mathieu Dubé[SUP] 2 [/SUP], Gérémy Sannier[SUP] 1 [/SUP], Alexandre Nicolas[SUP] 1 [/SUP], Lorie Marchitto[SUP] 1 [/SUP], Olivier Tastet[SUP] 2 [/SUP], Alexandra Tauzin[SUP] 1 [/SUP], Nathalie Brassard[SUP] 2 [/SUP], Raphaël Lima-Barbosa[SUP] 3 [/SUP], Guillaume Beaudoin-Bussières[SUP] 1 [/SUP], Dani Vézina[SUP] 2 [/SUP], Shang Yu Gong[SUP] 4 [/SUP], Mehdi Benlarbi[SUP] 2 [/SUP], Romain Gasser[SUP] 1 [/SUP], Annemarie Laumaea[SUP] 1 [/SUP], Jérémie Prévost[SUP] 1 [/SUP], Catherine Bourassa[SUP] 2 [/SUP], Gabrielle Gendron-Lepage[SUP] 2 [/SUP], Halima Medjahed[SUP] 2 [/SUP], Guillaume Goyette[SUP] 2 [/SUP], Gloria-Gabrielle Ortega-Delgado[SUP] 2 [/SUP], Mélanie Laporte[SUP] 2 [/SUP], Julia Niessl[SUP] 1 [/SUP], Laurie Gokool[SUP] 2 [/SUP], Chantal Morrisseau[SUP] 2 [/SUP], Pascale Arlotto[SUP] 2 [/SUP], Jonathan Richard[SUP] 1 [/SUP], Justin Bélair[SUP] 3 [/SUP], Alexandre Prat[SUP] 5 [/SUP], Cécile Tremblay[SUP] 1 [/SUP], Valérie Martel-Laferrière[SUP] 1 [/SUP], Andrés Finzi[SUP] 6 [/SUP], Daniel E Kaufmann[SUP] 7 [/SUP]
Affiliations
Abstract
Spacing of BNT162b2 mRNA doses beyond 3 weeks raises concerns about vaccine efficacy. We longitudinally analyze B cell, T cell, and humoral responses to two BNT162b2 mRNA doses administered 16 weeks apart in 53 SARS-CoV-2 naive and previously infected donors. This regimen elicits robust RBD-specific B cell responses whose kinetics differs between cohorts, the second dose leading to increased magnitude in naive participants only. While boosting does not increase magnitude of CD4[SUP]+[/SUP] T cell responses further compared with the first dose, unsupervised clustering of single-cell features reveals phenotypic and functional shifts over time and between cohorts. Integrated analysis shows longitudinal immune component-specific associations, with early T helper responses post first dose correlating with B cell responses after the second dose, and memory T helper generated between doses correlating with CD8 T cell responses after boosting. Therefore, boosting elicits a robust cellular recall response after the 16-week interval, indicating functional immune memory.
Keywords: AIM assay; B cells; CD4 T cell; CD4 T cell help; CD8 T cell; COVID-19; CP: Immunology; SARS-CoV-2; Spike glycoproteins; coronavirus; humoral responses; immunological memory; longer interval; mRNA vaccine; vaccine regimen.
. 2022 Jun 13;111013.
doi: 10.1016/j.celrep.2022.111013. Online ahead of print.
Temporal associations of B and T cell immunity with robust vaccine responsiveness in a 16-week interval BNT162b2 regimen
Manon Nayrac[SUP] 1 [/SUP], Mathieu Dubé[SUP] 2 [/SUP], Gérémy Sannier[SUP] 1 [/SUP], Alexandre Nicolas[SUP] 1 [/SUP], Lorie Marchitto[SUP] 1 [/SUP], Olivier Tastet[SUP] 2 [/SUP], Alexandra Tauzin[SUP] 1 [/SUP], Nathalie Brassard[SUP] 2 [/SUP], Raphaël Lima-Barbosa[SUP] 3 [/SUP], Guillaume Beaudoin-Bussières[SUP] 1 [/SUP], Dani Vézina[SUP] 2 [/SUP], Shang Yu Gong[SUP] 4 [/SUP], Mehdi Benlarbi[SUP] 2 [/SUP], Romain Gasser[SUP] 1 [/SUP], Annemarie Laumaea[SUP] 1 [/SUP], Jérémie Prévost[SUP] 1 [/SUP], Catherine Bourassa[SUP] 2 [/SUP], Gabrielle Gendron-Lepage[SUP] 2 [/SUP], Halima Medjahed[SUP] 2 [/SUP], Guillaume Goyette[SUP] 2 [/SUP], Gloria-Gabrielle Ortega-Delgado[SUP] 2 [/SUP], Mélanie Laporte[SUP] 2 [/SUP], Julia Niessl[SUP] 1 [/SUP], Laurie Gokool[SUP] 2 [/SUP], Chantal Morrisseau[SUP] 2 [/SUP], Pascale Arlotto[SUP] 2 [/SUP], Jonathan Richard[SUP] 1 [/SUP], Justin Bélair[SUP] 3 [/SUP], Alexandre Prat[SUP] 5 [/SUP], Cécile Tremblay[SUP] 1 [/SUP], Valérie Martel-Laferrière[SUP] 1 [/SUP], Andrés Finzi[SUP] 6 [/SUP], Daniel E Kaufmann[SUP] 7 [/SUP]
Affiliations
- PMID: 35732172
- DOI: 10.1016/j.celrep.2022.111013
Abstract
Spacing of BNT162b2 mRNA doses beyond 3 weeks raises concerns about vaccine efficacy. We longitudinally analyze B cell, T cell, and humoral responses to two BNT162b2 mRNA doses administered 16 weeks apart in 53 SARS-CoV-2 naive and previously infected donors. This regimen elicits robust RBD-specific B cell responses whose kinetics differs between cohorts, the second dose leading to increased magnitude in naive participants only. While boosting does not increase magnitude of CD4[SUP]+[/SUP] T cell responses further compared with the first dose, unsupervised clustering of single-cell features reveals phenotypic and functional shifts over time and between cohorts. Integrated analysis shows longitudinal immune component-specific associations, with early T helper responses post first dose correlating with B cell responses after the second dose, and memory T helper generated between doses correlating with CD8 T cell responses after boosting. Therefore, boosting elicits a robust cellular recall response after the 16-week interval, indicating functional immune memory.
Keywords: AIM assay; B cells; CD4 T cell; CD4 T cell help; CD8 T cell; COVID-19; CP: Immunology; SARS-CoV-2; Spike glycoproteins; coronavirus; humoral responses; immunological memory; longer interval; mRNA vaccine; vaccine regimen.